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Lack of association between ENAM gene polymorphism and dental caries in primary and permanent teeth in Czech children

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Abstract

Objectives

The enamelin gene (ENAM) polymorphism (rs12640848) was recently associated with dental caries in primary teeth in Polish children. The aims of the present study were to prove this association in primary dentition and to find a possible effect of this variant on caries development in permanent dentition in Czech children.

Materials and methods

This study comprised 905 Czech children. Totally, 187 children aged 2–6 years with primary dentition [78 healthy subjects (with decayed/missing/filled teeth, dmft = 0) and 109 patients with early childhood caries (ECC; dmft ≥ 1)] were included in this case-control study. In addition, 177 subjects aged 13–15 years without caries (DMFT = 0) and 541 children with dental caries (DMFT ≥ 1) in permanent dentition were selected from the ELSPAC study. Genotype determination of the ENAM polymorphism (rs12640848) was based on the TaqMan method.

Results

No significant differences in the allele or genotype frequencies between the caries-free children and those affected by dental caries were observed in both primary and permanent dentitions.

Conclusions

Lack of association between the ENAM polymorphism (rs12640848) and dental caries in Czech children was detected.

Clinical relevance

Although ENAM is considered as a candidate gene for dental caries, the presence of the ENAM variant (rs12640848) cannot be used as a risk factor of this multifactorial disease in the Czech population.

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Funding

The study was supported by funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, grants AZV 17-30439A, GACR GB14-37368G, projects MUNI/A/0948/2016 and Recetox RI (CZ.02.1.01/0.0/0.0/16_013/0001761).

Author information

Authors and Affiliations

  1. Clinic of Stomatology, Institution Shared with St. Anne’s Faculty Hospital, Faculty of Medicine, Masaryk University, Pekarska 664/53, 60200, Brno, Czech Republic

    Petra Borilova Linhartova, Tereza Deissova, Kristina Musilova, Martina Kukletova & Lydie Izakovicova Holla

  2. Department of Pathophysiology, Faculty of Medicine, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Petra Borilova Linhartova & Lydie Izakovicova Holla

  3. Stomatology Research Center, Faculty of Medicine, Masaryk University, Komenskeho namesti 220/2, 602 00, Brno, Czech Republic

    Lenka Zackova

  4. Research Centre for Toxic Compounds in the Environment (RECETOX), Chemistry Section, Faculty of Science, Masaryk University, Kamenice 5, 62500, Brno, Czech Republic

    Lubomir Kukla & Lydie Izakovicova Holla

Authors
  1. Petra Borilova Linhartova
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  2. Tereza Deissova
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  3. Kristina Musilova
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  4. Lenka Zackova
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  5. Martina Kukletova
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  6. Lubomir Kukla
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  7. Lydie Izakovicova Holla
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Contributions

PBL, MK, LK, and LIH designed the study and drafted the paper. KM, LZ, and MK performed the clinical analyses and collected blood or saliva samples. PBL and TD carried out the molecular analysis. LIH performed statistical analysis. All authors approved the final version of the manuscript.

Corresponding author

Correspondence to Lydie Izakovicova Holla.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Committee for Ethics of the Faculty of Medicine, MU Brno (3/2004, from 30/03/ 2004) and St. Anne’s Faculty Hospital (without number, from 13/04/2004 and 1G/2017).

Informed consent

In line with the Helsinki declaration, informed consent was obtained from all participants or legal representatives of children included in the study.

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Borilova Linhartova, P., Deissova, T., Musilova, K. et al. Lack of association between ENAM gene polymorphism and dental caries in primary and permanent teeth in Czech children. Clin Oral Invest 22, 1873–1877 (2018). https://doi.org/10.1007/s00784-017-2280-2

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  • DOI: https://doi.org/10.1007/s00784-017-2280-2

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