Clinical Oral Investigations

, Volume 21, Issue 7, pp 2319–2325 | Cite as

Correlation of crevicular fluid and serum levels of retinol-binding protein 4 and leptin in chronic periodontitis and obesity

  • Dharmendra Kanoriya
  • A R PradeepEmail author
  • A Mallika
  • Sandeep Singhal
  • Vibhuti Garg
Original Article



Retinol-binding protein 4 (RBP4) and leptin are both adipokines and involved in the pathophysiology of different vascular and inflammatory diseases and selectively elevated in patients with obesity. The aim of the present study was to determine and correlate the levels of RBP4 and leptin in gingival crevicular fluid (GCF) and serum in patients with chronic periodontitis (CP) and obesity.

Materials and methods

A total of 70 patients with age group 25 to 45 years were divided into four groups based on gingival index (GI), probing depth (PD), clinical attachment level (CAL), body mass index (BMI) and radiographic evidence of bone loss. The groups were (1) group I (non-obese periodontally healthy), (2) group II (obese periodontally healthy), (3) group III (non-obese with chronic periodontitis) and (4) group IV (obese with chronic periodontitis). The GCF and serum levels of human RBP4 and leptin were quantified using ELISA.


An increase in RBP4 levels from group I to group IV was found in both GCF and serum. However, GCF leptin levels was found to be greatest in group II, then group I, group IV and group III showing the least while an increase in serum levels from group I to group IV was found. The GCF and serum values of the inflammatory mediator correlated with the evaluated periodontal parameters and with each other (p < 0.05).


RBP4 and leptin can be considered as possible GCF and serum markers of inflammatory activity in CP and obesity, which further longitudinal studies are needed.


Chronic periodontitis Inflammation Obesity Gingival crevicular fluid 



The authors express their thanks to Mr. Jagannath, statistician, Bangalore, India, for statistical analysis performance.

Compliance with ethical standards

The study is in compliance with ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.


Authors declare no financial support or relationships that may pose conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Dharmendra Kanoriya
    • 1
  • A R Pradeep
    • 1
    Email author
  • A Mallika
    • 1
  • Sandeep Singhal
    • 1
  • Vibhuti Garg
    • 1
  1. 1.Department of PeriodontologyGovernment Dental College and Research InstituteBangaloreIndia

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