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Clinical Oral Investigations

, Volume 21, Issue 5, pp 1659–1665 | Cite as

Osteogenic potential of recombinant human bone morphogenetic protein-9/absorbable collagen sponge (rhBMP-9/ACS) in rat critical size calvarial defects

  • Toshiaki Nakamura
  • Yoshinori Shirakata
  • Yukiya Shinohara
  • Richard J. Miron
  • Kirara Furue
  • Kazuyuki NoguchiEmail author
Original Article

Abstract

Objectives

It has been reported that bone morphogenetic protein (BMP)-9 has potent osteoinductive properties among the BMP family by adenovirus-transfection experiments. We very recently reported that absorbable collagen sponge (ACS) as a carrier for recombinant human (rh) BMP-9, compared with chitosan sponge, was suitable for inducing bone healing/regeneration by BMP-9 in a rat calvarial defect model. The aim of this study was to evaluate different doses of rhBMP-9/ACS on new bone formation in rat critical size calvarial defects.

Materials and methods

Bilateral calvarial defects (n = 32) were surgically created in 16 wistar rats and randomly filled with one of the following materials: (1) absorbable collagen sponge (ACS) alone; (2) 1 μg-rhBMP-9/ACS (L-rhBMP-9/ACS); (3) 5 μg-rhBMP-9/ACS (H-rhBMP-9/ACS); and (4) blank defects (control). The animals were sacrificed 8 weeks postsurgery for radiographic and histomorphometric analyses.

Results

Bone volume and defect closure were statistically higher in the rhBMP-9/ACS-implanted (L-rhBMP-9/ACS and H-rhBMP-9/ACS) groups when compared with ACS-alone group (p < 0.05). Furthermore, defects filled with H-rhBMP-9/ACS showed the highest levels of newly formed bone area (NBA) and NBA/total defect area among all groups. No significant differences in any of the radiographic and histometric parameters could be observed between both concentrations of rhBMP-9.

Conclusions

Within the limits of this study, it can be concluded that rhBMP-9/ACS-induced bone formation can be reached with as little as 1 μg/site in rat critical size calvarial defects.

Clinical relevance

RhBMP-9 could be a potential therapeutic growth factor for future bone regenerative procedures.

Keywords

Growth factor Bone morphogenetic protein-9 Bone formation Collagen Osteoinduction 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interests.

Funding

This study was supported by Grants-in-Aid for Scientific Research (B) (no. 24792147) and (C) (no. 26462972) from the Japan Society for the Promotion of Science (JSPS).

Ethical approval

All animal experimental protocols and procedures were approved by the Ethical Committee of the Animal Research Center of Kagoshima University, Japan (D14022).

Informed consent

Informed consent was not required in this study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Toshiaki Nakamura
    • 1
  • Yoshinori Shirakata
    • 1
  • Yukiya Shinohara
    • 1
  • Richard J. Miron
    • 2
  • Kirara Furue
    • 1
  • Kazuyuki Noguchi
    • 1
    Email author
  1. 1.Department of PeriodontologyKagoshima University Graduate School of Medical and Dental SciencesKagoshimaJapan
  2. 2.Department of PeriodontologyNova Southeastern UniversityFort LauderdaleUSA

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