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Clinical Oral Investigations

, Volume 21, Issue 5, pp 1743–1752 | Cite as

Bacterial invasion into radicular dentine—an in vitro study

  • Simone Stauffacher
  • Adrian Lussi
  • Sandor Nietzsche
  • Klaus W. NeuhausEmail author
  • Sigrun Eick
Original Article

Abstract

Objectives

We wanted to investigate differences in invasiveness into radicular dentinal tubules by monocultured and co-cultured bacteria frequently found in infected root canals.

Methods

Fifty-one human roots were incubated for 8 weeks with monocultured Streptococcus gordonii ATCC 10558, Streptococcus sanguinis ATCC 10556, and with five capnophiles/anaerobes as well as with capnophiles/anaerobes co-cultured with a streptococcal species. Thereafter, bacterial samples were cultured from the inner, middle, and outer third of the root dentine of longitudinally broken teeth (n = 5). In addition, scanning electron microscopy (SEM) images were obtained.

Results

Single gram-positive species were able to penetrate into the middle and outer third of the root dentine. Fusobacterium nucleatum ATCC 25586 was not found in any of the dentine specimens. Prevotella intermedia ATCC 25611 and Porphyromonas gingivalis ATCC 33277 were found in the inner and middle third.

The bacterial load of streptococci was higher in all thirds in co-cultures compared to single infections. In co-cultures with streptococci, Actinomyces oris ATCC 43146 was found in the outer third in 9/10 samples, whereas P. intermedia ATCC 25611 was not detectable inside dentine. Co-culture with S. sanguinis ATCC 10556 enabled F. nucleatum ATCC 25586 to invade dentine; SEM images showed that F. nucleatum ATCC 25586 had a swollen shape.

Conclusions

Invasiveness of bacteria into dentinal tubules is species-specific and may change depending on culturing as a single species or co-culturing with other bacteria.

Clinical relevance

Oral streptococci may promote or inhibit invasion of capnophiles/anaerobes into radicular dentine.

Keywords

Bacterial invasion Root dentine Scanning electron microscopy Co-culture Monoculture Dentinal tubules 

Notes

Acknowledgments

The authors are grateful to Stéphanie Larti, Anna Magdon, and Marianne Weibel (University of Bern, Department of Periodontology, Laboratory of Oral Microbiology) for their technical assistance.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

The work was supported by the participating institutions.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

For this type of study, formal consent is not required.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Simone Stauffacher
    • 1
  • Adrian Lussi
    • 1
  • Sandor Nietzsche
    • 2
  • Klaus W. Neuhaus
    • 1
    Email author
  • Sigrun Eick
    • 3
  1. 1.Department of Preventive, Restorative and Pediatric DentistryUniversity of BernBernSwitzerland
  2. 2.Center of Electron MicroscopyUniversity Hospital of JenaJenaGermany
  3. 3.Laboratory of Oral Microbiology, Department of PeriodontologyUniversity of BernBernSwitzerland

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