Skip to main content

Advertisement

Log in

Impact of the intermediary layer on sealant retention: a randomized 24-month clinical trial

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

Objectives

The aims of this study were to assess long-term impact of tooth eruption stages (ES) on sealant retention on occlusal surfaces previously coated with intermediary bonding layer and to determine caries prevention.

Materials and methods

Sixty-five school children were selected (aged 6–10 years), with four non-carious permanent first molar in different ES (OP (operculum present), ME (marginal edge), CE (completely erupted)). Split-mouth and single-blind study design was used. The teeth (260) were randomly selected according to treatment (sealant/technique): F (Fluroshield), H (Helioseal Clear Chroma), SF (Single Bond + F), EH (Excite + H). Sealant retention, marginal integrity, discoloration, and caries prevention were assessed after 6, 12, 18, and 24 months by calibrated examiner (Spearman = 0.91) using visual inspection. Data were submitted to the Cox proportional hazard model (survival analysis) and Likelihood ratio χ 2 test (correlation), p ≤ 0.05.

Results

At baseline, ES was 20 % in OP, 54 % in ME, and 26 % in CE. There was no significant difference on sealant retention between the treatments (p = 0.2774). However, significant differences were found regarding the ES on sealant retention (p = 0.0041). The CE stage showed the highest retention survival rate during the 24 months. The overall sealant prevention average was found to be about 99.4 % and showed no difference between the groups.

Conclusions

Eruption stages affect sealant retention irregardless of the intermediate layer and type of sealant. However, there was caries prevention on tooth occlusal surfaces after 24 months, regardless of treatment.

Clinical relevance

Sealing is recommended to prevent occlusal caries of newly erupted teeth in high-caries-risk patients. However, its application is critical on moisture limited control surfaces independently of sealer material and technique.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Jodkowska E (2008) Efficacy of pit and fissure sealing: long-term clinical observations. Quintessence Int 39:593–602

    PubMed  Google Scholar 

  2. Jurić H (2013) Current possibilities in occlusal caries management. Acta Med Acad 42:216–222

    Article  PubMed  Google Scholar 

  3. Hebling J, Feigal RJ (2000) Use of one-bottle adhesive as an intermediate bonding layer to reduce sealant microleakage on saliva-contaminated enamel. Am J Dent 13:187–191

    PubMed  Google Scholar 

  4. Lynch RJM (2013) The primary and mixed dentition, post-eruptive enamel maturation and dental caries: a review. Int Dent J 63:3–13

    Article  PubMed  Google Scholar 

  5. Ahovuo-Saloranta A, Hiiri A, Nordblad A, Mäkelä M, Worthington H (2008) Pit and fissure sealants for preventing dental decay in the permanent teeth of children and adolescents. Cochrane Database Syst Rev 4:CD001830

    Google Scholar 

  6. Splieth CH, Ekstrand KR, Alkilzy M, Clarkson J, Meyer-Lueckel H, Martignon S, et al. (2010) Sealants in dentistry: outcomes of the ORCA Saturday Afternoon Symposium 2007. Caries Res 44:3–13

    Article  PubMed  Google Scholar 

  7. Ahovuo-Saloranta A, Forss H, Walsh T, Hiiri A, Nordblad A, Mäkelä M, et al. (2013) Sealants for preventing dental decay in the permanent teeth. Cochrane Libr 28:1–156

    Google Scholar 

  8. Dennison JB, Straffon LH, More FG (1990) Evaluating tooth eruption on sealant efficacy. J Am Dent Assoc 121:610–614

    Article  PubMed  Google Scholar 

  9. Feigal RJ, Hitt J, Splieth C (1993) Retaining sealant on salivary contaminated enamel. J Am Dent Assoc 124:88–97

    Article  PubMed  Google Scholar 

  10. Nogourani MK, Janghorbani M, Khadem P, Jadidi Z, Jalali S (2012) A 12-month clinical evaluation of pit-and-fissure sealants placed with and without etch-and-rinse and self-etch adhesive systems in newly-erupted teeth. J Appl Oral Sci 20:352–356

    Article  PubMed  PubMed Central  Google Scholar 

  11. Carvalho JC (2014) Caries process on occlusal surfaces: evolving evidence and understanding. Caries Res 48:339–346

    Article  PubMed  Google Scholar 

  12. Silverstone LM, Hicks MJ, Featherstone MJ (1985) Oral fluid contamination of etched enamel surfaces: an SEM study. J Am Dent Assoc 110:329–332

    Article  PubMed  Google Scholar 

  13. Hitt JC, Feigal RJ (1992) Use of a bonding agent to reduce sealant sensitivity to moisture contamination: an in vitro study. Pediatr Dent 14:41–46

    PubMed  Google Scholar 

  14. Askarizadeh N, Norouzi N, Nemati S (2008) The effect of bonding agents on the microleakage of sealant following contamination with saliva. J Indian Soc Pedodontics Prev Dent 26:64–66

    Article  Google Scholar 

  15. Feigal RJ, Musherure P, Gillespie B, Levy-Polack M, Quelhas I, Hebling J (2000) Improved sealant retention with bonding agents: a clinical study of two-bottle and single-bottle systems. J Dent Res 79:1850–1856

    Article  PubMed  Google Scholar 

  16. Burbridge L, Nugent Z, Deery C (2006) A randomized controlled trial of the effectiveness of a one-step conditioning agent in sealant placement: 6-month results. Int J Paediatr Dent 16:424–430

    Article  PubMed  Google Scholar 

  17. Burbridge L, Nugent Z, Deery C (2007) A randomized controlled trial of the effectiveness of a one-step conditioning agent in fissure sealant placement: 12 month results. Eur Arch Paediatr Dent 8:49–54

    Article  PubMed  Google Scholar 

  18. Lygidakis NA, Dimou G, Stamataki E (2009) Retention of fissure sealants using two different methods of application in teeth with hypomineralised molars (MIH): a 4 year clinical study. Eur Arch Paediatr Dent 10:223–226

    Article  PubMed  Google Scholar 

  19. Pinar A, Sepet E, Aren G, Bölükbaşi N, Ulukapi H, Turan N (2005) Clinical performance of sealants with and without a bonding agent. Quintessence Int 36:355–360

    PubMed  Google Scholar 

  20. Cury JA, Lima YBO (2001) Ingestão de flúor por crianças pela água e dentifrício fluoride intake by children from water and dentifrice. Rev Saude Publica 35:576–581

    Article  PubMed  Google Scholar 

  21. Mascarenhas AK, Nazar H, Al-Mutawaa S, Soparkar P (2008) Effectiveness of primer and bond in sealant retention and caries prevention. Pediatr Dent 30:25–28

    PubMed  Google Scholar 

  22. Nazar H, Mascarenhas AK, Al-Mutwa S, Ariga J, Soparker P (2013) Effectiveness of fissure sealant retention and caries prevention with and without primer and bond. Med Princ Pract 22:12–17

    Article  PubMed  Google Scholar 

  23. Boksman L, McConnell RJ, Carson B, McCutcheon-Jones EF (1993) A 2-year clinical evaluation of two pit and fissure sealants placed with and without the use of a bonding agent. Quintessence Int 24:131–133

    PubMed  Google Scholar 

  24. Locker D, Jokovic A, Kay EJ (2003) Prevention. Part 8: the use of pit and fissure sealants in preventing caries in the permanent dentition of children. Br Dent J 195:375–378

    Article  PubMed  Google Scholar 

  25. Biria M, Ghasemi A, Torabzadeh H, Shisheeian A, Baghban A (2014) Assessment of microshear bond strength: self-etching sealant versus conventional sealant. J Dent 11:137–142

    Google Scholar 

  26. Bhat PK, Konde S, Raj SN, Kumar NC (2013) Moisture-tolerant resin-based sealant: a boon. Contemp Clin Dent 4:343–348

    Article  PubMed  PubMed Central  Google Scholar 

  27. Koch MJ, García-Godoy F, Mayer T, Staehle HJ (1997) Clinical evaluation of Helioseal F fissure sealant. Clin Oral Investig 1:199–202

    Article  PubMed  Google Scholar 

  28. Heifetz SB, Yaari A, Proskin HM (2004) Retention of a fluoride-releasing sealant compared with its non-fluoride analogue: interim results of a clinical study after an average of eight months. J Clin Dent 15:1–5

    PubMed  Google Scholar 

  29. Dukic W, Glavina D (2007) Clinical evaluation of three fissure sealants: 24 month follow-up. Eur Arch Paediatr Dent 8:163–166

    Article  PubMed  Google Scholar 

  30. Kargul B, Tanboga I, Gulman N (2009) A comparative study of fissure sealants Helioseal Clear Chroma and Delton FS(+): 3 year results. Eur Arch Paediatr Dent 10:218–222

    Article  PubMed  Google Scholar 

  31. Reddy VR, Chowdhary N, Mukunda KS, Kiran NK, Kavyarani BS, Pradeep MC (2015) Retention of resin-based filled and unfilled pit and fissure sealants: a comparative clinical study. Contemp Clin Dent 6:18–23

    Article  Google Scholar 

  32. Feigal RJ, Quelhas I (2003) Clinical trial of a self-etching adhesive for sealant application: success at 24 months with Prompt L-Pop. Am J Dent 16:249–251

    PubMed  Google Scholar 

  33. Subramaniam P, Konde S, Mandanna DK (2008) Retention of a resin-based sealant and a glass ionomer used as a fissure sealant: a comparative clinical study. J Ind Soc Pedodontics Prev Dent 26:114–120

    Article  Google Scholar 

  34. Feigal RJ (2002) The use of pit and fissure sealants. Pediatr Dent 24:415–422

    PubMed  Google Scholar 

  35. Hoffman I (2009) A moisture tolerant, resin-based pit and fissure sealant. Dent Tribune 1:17–18

    Google Scholar 

  36. Guler C, Yilmaz Y (2013) A two-year clinical evaluation of glass ionomer and ormocer based fissure sealants. J Clin Pediatr Dent 37:263–267

    Article  PubMed  Google Scholar 

  37. Antonson SA, Antonson DE, Brener S, Crutchfield J, Larumbe J, Michaud C, et al. (2012) Twenty-four month clinical evaluation of fissure sealants on partially erupted permanent first molars: glass ionomer versus resin-based sealant. J Am Dent Assoc 143:115–122

    Article  PubMed  Google Scholar 

  38. Vermaire JH, van Loveren C, Brouwer WB, Krol M (2015) The cost-effectiveness evaluation of 2 caries prevention strategies compared with the standard approach. Ned Tijdschr Tandheelkunde 122:392–401

    Article  Google Scholar 

  39. Dhar V, Chen H (2012) Evaluation of resin based and glass ionomer based sealants placed with or without tooth preparation—a two year clinical trial. Pediatr Dent 34:46–50

    PubMed  Google Scholar 

  40. Oba AA, Sönmez IŞ, Ercan E, Dülgergil T (2012) Comparison of retention rates of fissure sealants using two flowable restorative materials and a conventional resin sealant: two-year follow-up. Med Princ Pract 21:234–237

    Article  PubMed  Google Scholar 

  41. Ünal M, Oznurhan F, Kapdan A, Dürer S (2015) A comparative clinical study of three fissure sealants on primary teeth: 24-month results. J Clin Pediatr Dent 39:113–119

    Article  PubMed  Google Scholar 

  42. Kantovitz KR, Pascon FM, Correr GM, Alonso RC, Rodrigues LK, Alves MC, et al. (2009) Influence of environmental conditions on properties of ionomeric and resin sealant materials. J Appl Oral Sci 17:294–300

    Article  PubMed  PubMed Central  Google Scholar 

  43. Berger S, Goddon I, Chen CM, Senkel H, Hickel R, Stösser L, et al. (2010) Are pit and fissure sealants needed in children with a higher caries risk? Clin Oral Investig 14:613–620

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Department of Pediatric Dentistry, State University of Campinas, Piracicaba Dental School. Specifically, our thanks extend to Mr. Norm Schiff for his cooperation and assistance in text editing and the Writing Area/General Coordination of the State University of Campinas-UNICAMP. This research was supported by CAPES–Coordination for the Improvement of Higher Education Personnel.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Regina Maria Puppin-Rontani.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

This was supported by the Coordination for the Improvement of Higher Education Personnel (CAPES) in Piracicaba, São Paulo.

Ethical approval

This article does contain any studies with human participants performed by any of the authors. This clinical trial study was conducted after approval from the Ethics Committee of Piracicaba Dental School, State University of Campinas (protocol number 143/2003).

Informed consent

For this type of study, formal consent is required. Thus, informed consent was obtained from all individual participants included in the study.

Additional information

Kelly Maria Silva Moreira and Kamila Rosamilia Kantovitz contributed equally.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Moreira, K.M.S., Kantovitz, K.R., Aguiar, J.P.D. et al. Impact of the intermediary layer on sealant retention: a randomized 24-month clinical trial. Clin Oral Invest 21, 1435–1443 (2017). https://doi.org/10.1007/s00784-016-1890-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-016-1890-4

Keywords

Navigation