Clinical Oral Investigations

, Volume 20, Issue 8, pp 2083–2095 | Cite as

Salivary function impairment in type 2 Diabetes patients associated with concentration and genetic polymorphisms of chromogranin A

  • Evelyn Mikaela KogawaEmail author
  • Daniela Corrêa Grisi
  • Denise Pinheiro Falcão
  • Ingrid Aquino Amorim
  • Taia Maria Berto Rezende
  • Izabel Cristina Rodrigues da Silva
  • Osmar Nascimento Silva
  • Octávio Luiz Franco
  • Rivadávio Fernandes Batista de Amorim
Original Article



The purpose of this study was to evaluate the effect of type 2 diabetes mellitus (T2DM) on salivary function impairments according to glycemic control status and subsequently compare the concentration of chromogranin A (CHGA) with its genetic profile.

Materials and methods

Thirty-six patients with controlled T2DM, 36 with poorly controlled T2DM, and 38 nondiabetic subjects underwent salivary flow rate measurements by means of unstimulated labial (ULS), unstimulated whole (UWS), and stimulated whole saliva (SWS) collections. CHGA concentrations were determined in saliva and plasma with ELISA, and two CHGA polymorphisms (T-415C and Glu264Asp) were analyzed by polymerase chain reaction-restriction fragment length polymorphism.


T2DM patients presented significantly lower ULS and UWS flow rates regardless of glycemic control status compared to controls (P = 0.002 and P = 0.027, respectively). The SWS flow rate in the poorly controlled T2DM was the lowest among the groups (P = 0.026). Significantly higher plasma and salivary CHGA levels were found in T2DM groups (P = 0.019 and P < 0.001, respectively). CHGA gene variants (T-415C and Glu264Asp) revealed significant differences between diabetics and control subjects when associated with lower salivary flow and higher salivary CHGA production (P < 0.05).


T2DM causes abnormalities in the function of salivary glands. However, poorly controlled T2DM has the most influence on SWS flow rates. Our findings indicate an association between plasma and salivary CHGA levels and T2DM patients. Furthermore, the results suggest that CGHA polymorphisms might be associated with salivary gland hypofunction and higher salivary CHGA production in T2DM patients. Nevertheless, further epidemiological studies are required to elucidate this clinical implication.

Clinical relevance

Salivary impairments and high levels of CHGA are associated with T2DM patients. In addition, CGHA polymorphisms might be associated with salivary gland hypofunction and higher salivary CHGA production in T2DM patients. This could be a significant insight to establish a role for salivary CHGA as a potential clinical biomarker to T2DM.


Type 2 diabetes Chromogranin A Saliva Polymorphism Genetic 



The present study would not have been possible without the participation of the patients and healthy volunteers. The authors would also like to thank Isabel Cristina Marques Fensterseifer, Larissa Bittencourt, Selma Soares Nascimento, Diego Batista da Silva, and Poliana Amanda Oliveira Silva for their excellent help with the experiments.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


This study was supported by grants from the Universidade de Brasília (DPP 10/2011) and Universidade Católica de Brasília (UCB).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Evelyn Mikaela Kogawa
    • 1
    • 2
    • 9
    Email author
  • Daniela Corrêa Grisi
    • 2
  • Denise Pinheiro Falcão
    • 1
  • Ingrid Aquino Amorim
    • 3
    • 4
  • Taia Maria Berto Rezende
    • 2
    • 3
    • 4
  • Izabel Cristina Rodrigues da Silva
    • 5
  • Osmar Nascimento Silva
    • 4
    • 6
  • Octávio Luiz Franco
    • 4
    • 6
    • 7
    • 8
  • Rivadávio Fernandes Batista de Amorim
    • 1
  1. 1.Programa de Pós-graduação em Ciências MédicasUniversidade de BrasíliaBrasíliaBrazil
  2. 2.Curso de OdontologiaUniversidade Católica de BrasíliaBrasíliaBrazil
  3. 3.Programa de Pós-Graduação em Ciências da SaúdeUniversidade de BrasíliaBrasíliaBrazil
  4. 4.Centro de Análises Proteômicas e Bioquímicas, Pós-graduação em Ciências Genômicas e BiotecnologiaUniversidade Católica de BrasíliaBrasíliaBrazil
  5. 5.FCE, Universidade de BrasíliaBrasíliaBrazil
  6. 6.Pós-graduação em Ciências Biológicas (Biotecnologia e Genética), Departamento de Biologia, Instituto de Ciências BiológicasUniversidade de Juiz de ForaJuiz de ForaBrazil
  7. 7.Programa de Doutorado da Rede Centro-OesteBrasíliaBrazil
  8. 8.S-Inova, Pós-Graduação em BiotecnologiaUniversidade Católica Dom BoscoCampo GrandeBrazil
  9. 9.Universidade Católica de BrasíliaBrasíliaBrazil

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