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Clinical Oral Investigations

, Volume 20, Issue 8, pp 1921–1933 | Cite as

Synthesis and characterization of CaO-loaded electrospun matrices for bone tissue engineering

  • Eliseu A. Münchow
  • Divya Pankajakshan
  • Maria T. P. Albuquerque
  • Krzysztof Kamocki
  • Evandro Piva
  • Richard L. Gregory
  • Marco C. BottinoEmail author
Original Article

Abstract

Objectives

This study aims to synthesize and characterize biodegradable polymer-based matrices loaded with CaO nanoparticles for osteomyelitis treatment and bone tissue engineering.

Materials and methods

Poly(ε-caprolactone) (PCL) and PCL/gelatin (1:1, w/w) solutions containing CaO nanoparticles were electrospun into fibrous matrices. Scanning (SEM) and transmission (TEM) electron microscopy, Fourier transformed infrared (FTIR), energy dispersive X-ray spectroscopy (EDS), contact angle (CA), tensile testing, and antibacterial activity (agar diffusion assay) against Staphylococcus aureus were performed. Osteoprecursor cell (MC3T3-E1) response (i.e., viability and alkaline phosphatase expression/ALP) and infiltration into the matrices were evaluated.

Results

CaO nanoparticles were successfully incorporated into the fibers, with the median fiber diameter decreasing after CaO incorporation. The CA decreased with the addition of CaO, and the presence of gelatin made the matrix very hydrophilic (CA = 0°). Increasing CaO concentrations progressively reduced the mechanical properties (p ≤ 0.030). CaO-loaded matrices did not display consistent antibacterial activity. MC3T3-E1 cell viability demonstrated the highest levels for CaO-loaded matrices containing gelatin after 7 days in culture. An increased ALP expression was consistently seen for PCL/CaO matrices when compared to PCL and gelatin-containing counterparts.

Conclusions

Despite inconsistent antibacterial activity, CaO nanoparticles can be effectively loaded into PCL or PCL/gelatin fibers without negatively affecting the overall performance of the matrices. More importantly, CaO incorporation enhanced cell viability as well as differentiation capacity, as demonstrated by an increased ALP expression.

Clinical significance

CaO-loaded electrospun matrices show potential for applications in bone tissue engineering.

Keywords

CaO Nanofibers Osteomyelitis Electrospinning Bacteria MC3T3-E1 

Notes

Acknowledgments

We acknowledge the expert assistance of Dr. Maria Malgorzata Kamocka with the confocal/2-photon imaging at the Indiana Center for Biological Microscopy, Indianapolis, IN.

Compliance with ethical standards

Funding

This study was funded in part by an International Development Funds (IDF) Grant from Indiana University Purdue University (IUPUI/OVCR), by start-up funds from the IU School of Dentistry, and the NIH-NIDCR (Grant # DE023552) (all to M.C.B.). In addition, this project was supported by the Indiana Clinical and Translational Sciences Institute, funded in part by grant #UL1 TR001108 from the National Institutes of Health, National Center for Advancing Translational Sciences, Clinical and Translational Sciences Award. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Eliseu A. Münchow
    • 1
    • 2
  • Divya Pankajakshan
    • 1
  • Maria T. P. Albuquerque
    • 1
    • 3
  • Krzysztof Kamocki
    • 1
  • Evandro Piva
    • 2
  • Richard L. Gregory
    • 1
  • Marco C. Bottino
    • 1
    Email author
  1. 1.Division of Dental Biomaterials, Department of Biomedical and Applied SciencesIndiana University School of Dentistry IndianapolisUSA
  2. 2.School of DentistryFederal University of Pelotas – UFPelPelotasBrazil
  3. 3.Graduate Program in DentistryUniversidade Estadual Paulista, São José dos Campos Dental SchoolSão José dos CamposBrazil

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