Clinical Oral Investigations

, Volume 20, Issue 3, pp 589–595 | Cite as

Bone grafting material in combination with Osteogain for bone repair: a rat histomorphometric study

  • Yufeng ZhangEmail author
  • Dai Jing
  • Daniel Buser
  • Anton Sculean
  • Fatiha Chandad
  • Richard J. MironEmail author
Original Article



Enamel matrix derivative (EMD) has been successfully used for the regeneration of periodontal tissues including new cementum, periodontal ligament, and alveolar bone. Combination of EMD with bone grafting materials has however generated variable clinical results. Recently, we have demonstrated that a new formulation of EMD in a liquid carrier system (Osteogain®) has improved physicochemical properties for the adsorption of EMD to a bone grafting material. The aim of the present study was to investigate the regenerative potential of Osteogain®, in combination with a bone graft, on new bone formation in a rat femur defect model.

Materials and methods

Fifty-four critically sized femur defects (3 mm in diameter) were created bilaterally in 27 rats and treated following the group allocation: (1) drilled unfilled control, (2) a natural bone mineral (NBM), and (3) NBM + Osteogain®. All defects were histologically analyzed at 2, 4, and 8 weeks after surgical intervention. Micro-CT analysis, hematoxylin and eosin (H&E) staining, and Safranin O staining were performed to quantify new bone formation.


Significantly more new bone formation was observed in defects treated with NBM + Osteogain® at both 4 and 8 weeks when compared to NBM alone and the control unfilled defects (P < 0.05). Histologically, the formation of more mature mineralized bone with the presence of osteocytes were found more commonly in defects treated with Osteogain® + NBM at 8 weeks post-healing when compared to NBM alone.


The present study demonstrate that Osteogain® in combination with a bone grafting material improves the speed and quality of new bone formation in rat osseous defects.

Clinical relevance

Future clinical research are now warranted to fully characterize the benefits of Osteogain®, a new formulation of enamel matrix proteins delivered in liquid formation when used in combination with a bone grafting material.


Enamel matrix derivative (EMD) Emdogain Natural bone mineral BioOss Bone grafting materials 


Conflict of interest

The study was funded by Straumann AG, Basel, Switzerland. The NBM bone graft was provided by Geistlich Pharma AG, Switzerland, and Osteogain® was provided by Straumann AG, Switzerland. No further conflict of interest exists.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of StomatologyWuhan UniversityWuhanChina
  2. 2.Department of Oral Surgery and Stomatology, School of Dental MedicineUniversity of BernBernSwitzerland
  3. 3.Department of Periodontology, School of Dental MedicineUniversity of BernBernSwitzerland
  4. 4.University of Laval, Dental SchoolQuebec CityCanada

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