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Clinical Oral Investigations

, Volume 19, Issue 5, pp 1063–1068 | Cite as

Reproducibility of subgingival bacterial samples from patients with peri-implant mucositis

  • Hadar Hallström
  • G. Rutger Persson
  • Ulf Strömberg
  • Svante Twetman
  • Stefan Renvert
Original Article

Abstract

Objective

The aim of the present study was to investigate the reproducibility of bacterial enumeration from subsequent subgingival samples collected from patients with peri-implant mucositis.

Material and methods

Duplicate microbial samples from 222 unique implant sites in 45 adult subjects were collected with paper points and analyzed using the checkerboard DNA-DNA hybridization technique. Whole genomic probes of 74 preselected bacterial species were used. Based on the bacterial scores, Cohen’s kappa coefficient was used to calculate the inter-annotator agreement for categorical data. The percentage agreement was considered as “good” when the two samples showed the same score or differed by 1 to the power of 10.

Results

Moderate to fair kappa values were displayed for all bacterial species in the test panel (range 0.21–0.58). There were no significant differences between Gram-positive and Gram-negative species. The percentage of good agreement between the first and second samples averaged 74.7 % (n = 74; range 56–83 %), while the proportion of poor agreement ranged from 1 to 19 % for the various strains.

Conclusion

While an acceptable clinical agreement was obtained in most cases, diverging bacterial scores may appear in subgingival samples collected at the same time point from patients with peri-implant mucositis.

Clinical relevance

The broad bulky base of implant crowns may present an obstacle for the collection of reproducible subgingival samples with paper points.

Keywords

Microbiology Diagnosis Clinical assessment Peri-implant mucositis DNA-DNA hybridization 

Notes

Acknowledgments

The authors are grateful to Mrs. Susann Lindgren, RDH, Halland Hospital, for her skilled clinical work. We also appreciate the work performed by Ms. Marianne Weibel and Ms. Regula Hirschi-Imfeld at the Department of Periodontology, Division of Oral Microbiology, School of Dental Medicine, University of Bern, Switzerland, for the processing of the microbiological material. The study was funded by research grants from Kristianstad University, Sweden.

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

References

  1. 1.
    Lindhe J, Meyle J, Group D et al (2008) Peri-implant diseases: consensus report of the sixth European workshop on periodontology. J Clin Periodontol 35:282–285CrossRefPubMedGoogle Scholar
  2. 2.
    Quirynen M, Vogels R, Peeters W, van Steenberghe D, Naert I, Haffajee A (2006) Dynamics of initial subgingival colonization of ‘pristine’ peri-implant pockets. Clin Oral Implants Res 17:25–37CrossRefPubMedGoogle Scholar
  3. 3.
    Furst MM, Salvi GE, Lang NP, Persson GR (2007) Bacterial colonization immediately after installation on oral titanium implants. Clin Oral Implants Res 18:501–508CrossRefPubMedGoogle Scholar
  4. 4.
    Charalampakis G, Leonhardt A, Rabe P, Dahlen G (2012) Clinical and microbiological characteristics of peri-implantitis cases: a retrospective multicentre study. Clin Oral Implants Res 23:1045–1054CrossRefPubMedGoogle Scholar
  5. 5.
    Charalampakis G, Rabe P, Leonhardt A, Dahlen G (2011) A follow-up study of peri-implantitis cases after treatment. J Clin Periodontol 38:864–871CrossRefPubMedGoogle Scholar
  6. 6.
    Persson GR, Renvert S (2013) Cluster of bacteria associated with peri-implantitis. Clin Implant Dent Relat Res. doi: 10.1111/cid.12052 Google Scholar
  7. 7.
    Persson GR, Roos-Jansaker AM, Lindahl C, Renvert S (2011) Microbiologic results after non-surgical erbium-doped: yttrium, aluminum, and garnet laser or air-abrasive treatment of peri-implantitis: a randomized clinical trial. J Periodontol 82:1267–1278CrossRefPubMedGoogle Scholar
  8. 8.
    Hallstrom H, Persson GR, Lindgren S, Olofsson M, Renvert S (2012) Systemic antibiotics and debridement of peri-implant mucositis. A randomized clinical trial. J Clin Periodontol 39:574–581CrossRefPubMedGoogle Scholar
  9. 9.
    Papapanou PN, Madianos PN, Dahlén G, Sandros J (1997) “Checkerboard” versus culture: a comparison between two methods for identification of subgingival microbiota. Eur J Oral Sci 105:389–396CrossRefPubMedGoogle Scholar
  10. 10.
    Gerber J, Wenaweser D, Heitz-Mayfield L, Lang NP, Persson GR (2006) Comparison of bacterial plaque samples from titanium implant and tooth surfaces by different methods. Clin Oral Implants Res 17:1–7CrossRefPubMedGoogle Scholar
  11. 11.
    Teles FR, Haffajee AD, Socransky SS (2008) The reproducibility of curet sampling of subgingival biofilms. J Periodontol 79:705–713CrossRefPubMedGoogle Scholar
  12. 12.
    Persson GR, Weibel M, Hirschi R, Katsoulis J (2008) Similarities in the subgingival microbiota assessed by a curet sampling method at sites with chronic periodontitis. J Periodontol 79:2290–2296CrossRefPubMedGoogle Scholar
  13. 13.
    Socransky SS, Haffajee AD, Smith C, Martin L, Haffajee JA, Uzel NG, Goodson JM (2004) Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol 19:352–362CrossRefPubMedGoogle Scholar
  14. 14.
    Altman DG (1991) Statistical analysis of comparison between laboratory methods. J Clin Pathol 44:700–701CrossRefPubMedCentralPubMedGoogle Scholar
  15. 15.
    Persson GR, Hitti J, Paul K, Hirschi R, Weibel M, Rothen M, Persson RE (2008) Tannerella forsythia and pseudomonas aeruginosa in subgingival bacterial samples from parous women. J Periodontol 79:508–516CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Hadar Hallström
    • 1
  • G. Rutger Persson
    • 3
    • 4
    • 5
  • Ulf Strömberg
    • 2
  • Svante Twetman
    • 6
  • Stefan Renvert
    • 3
    • 7
    • 8
  1. 1.Department of Periodontology, Faculty of OdontologyMalmö UniversityMalmöSweden
  2. 2.Department of Research and DevelopmentHalland HospitalHalmstadSweden
  3. 3.Department of Oral SciencesKristianstad UniversityKristianstadSweden
  4. 4.Department of Periodontics and Department of Oral MedicineUniversity of WashingtonSeattleUSA
  5. 5.Department of PeriodontologyUniversity of BernBernSwitzerland
  6. 6.Department of Odontology, Section for Cariology. Endodontics, Pediatric Dentistry and Clinical Genetics, Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark
  7. 7.Blekinge Institute of TechnologyKarlskronaSweden
  8. 8.School of Dental SciencesTrinity CollegeDublinIreland

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