Synchrotron-based X-ray tomographic microscopy for visualization of three-dimensional collagen matrices
- 494 Downloads
Three-dimensional collagen matrices (3D-CMs) may be visualized by cumbersome reconstructions of serial sections. We report here on the method of synchrotron-based X-ray tomographic microscopy (SRXTM) to image 3D-CMs in native tissue probes.
Material and methods
SRXTM of 3D-CMs (mucoderm®, mucograft®) was performed at the TOMCAT beamline of the Swiss Light Source (SLS) at the Paul Scherrer Institute (Villigen, Switzerland).
SRXTM combines the advantages of high-resolution scanning electron microscopy (SEM) imaging with the low-resolution reconstructions of micro-CT (μCT) imaging. It may be used to non-destructively visualize and analyze structures within the 3D-CMs without the need of serial sectioning and reconstruction.
High-resolution SRXTM is a useful tool in analyzing the topology and morphometry of structures in 3D-CMs. The outcome justifies the efforts in sophisticated data processing.
SRXTM may help to understand the clinical characteristics of 3D-CMs in more detail.
KeywordsSynchrotron radiation 3D visualization Morphometry Collagen matrix
Synchrotron-based X-ray tomographic microscopy (SRXTM) experiments were facilitated by a research grant of the Swiss Light Source at the Paul Scherrer Institute (Villigen, Switzerland) to A.M. Pabst. The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 312284 for CALIPSO.
Conflict of interests
The authors declare no conflict of interests.
- 3.Stampanoni M, Groso A, Isenegger A, Mikuljan G, Chen Q, Bertrand A, Henein S, Betemps R, Frommherz U, Böhler P, Meister D, Lange M, Abela R (2006) Trends in synchrotron-based tomographic imaging: the SLS experience. Proc. SPIE 6318, Developments in X-Ray Tomography V, 63180MGoogle Scholar