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Clinical Oral Investigations

, Volume 18, Issue 6, pp 1647–1654 | Cite as

Oral lichen planus may enhance the expression of Th17-associated cytokines in local lesions of chronic periodontitis

  • Hui Wang
  • Qi Han
  • Zhenhua Luo
  • Caixia Xu
  • Jiajia Liu
  • Hongxia Dan
  • Yi XuEmail author
  • Xin ZengEmail author
  • Qianming Chen
Original Article

Abstract

Objectives

This study aims to compare the expression levels of interleukin (IL)-17 and IL-23 in local periodontal tissues from patients with both chronic periodontitis and oral lichen planus (CP-OLP), patients with chronic periodontitis (CP) only, patients with oral lichen planus (OLP) only, and healthy controls (HC).

Materials and methods

The periodontal tissues were collected from 15 CP-OLP patients, 15 CP patients, 15 OLP patients, and 10 healthy controls. Immunohistochemistry (IHC) and real-time quantitative PCR (qPCR) was performed to investigate the protein and mRNA expression level of IL-17 and IL-23 in periodontal lesions from these four groups.

Results

IHC statistical analysis showed that the expression level of IL-17- and IL-23p19-positive cells significantly increased in CP-OLP group compared with that in CP (P < 0.01) and OLP groups (P < 0.05), showing intense staining reaction in local lamina propria lesions. Meanwhile, qPCR result showed higher IL-17 mRNA level in CP-OLP compared with that in CP and OLP groups and demonstrated a significant increase than OLP group (P < 0.05). Moreover, it was found that IL-17 mRNA expression level in erosive CP-OLP patients was significantly correlated with probing depth and attachment loss (P < 0.05).

Conclusions

This study indicated that there was an increased expression level of IL-17 and IL-23 in periodontal tissues from periodontitis patients with oral lichen planus, which might aggravate the inflammatory response in local lesions.

Clinical relevance

Oral lichen planus and chronic periodontitis may have interaction in disease pathogenesis, while IL-17 detection in local lesions may be helpful in identifying the disease severity in periodontitis patients with oral lichen planus.

Keywords

Oral lichen planus Periodontitis Interleukin (IL)-17 IL-23 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation (nos. 81200791, 81072218, 30872872, and 30930100) and the Doctoral Program of the Ministry of Education of China (nos. 20110181110055 and 20120181120011).

Conflicts of interest

The authors have no competing financial interests to declare.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hui Wang
    • 1
    • 2
  • Qi Han
    • 2
  • Zhenhua Luo
    • 2
  • Caixia Xu
    • 2
  • Jiajia Liu
    • 2
  • Hongxia Dan
    • 2
  • Yi Xu
    • 2
    Email author
  • Xin Zeng
    • 2
    Email author
  • Qianming Chen
    • 2
  1. 1.Department of Oral Medicine, School of StomatologyCapital Medical UniversityBeijingChina
  2. 2.State Key Laboratory of Oral Diseases, West China Hospital of StomatologySichuan UniversityChengduPeople’s Republic of China

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