Clinical Oral Investigations

, Volume 18, Issue 3, pp 873–881 | Cite as

A novel oligopeptide simulating dentine matrix protein 1 for biomimetic mineralization of dentine

  • Ying Cao
  • Wei Liu
  • Tianyun Ning
  • May L. Mei
  • Quan-Li Li
  • Edward C. M. Lo
  • C. H. Chu
Original Article



The objectives were to design and fabricate an oligopeptide that simulates dentine matrix protein 1 (DMP1) to study its ability to bind to dentine collagen fibrils and induce biomimetic mineralization for the management of dentine hypersensitivity.

Materials and methods

A novel oligopeptide was developed by connecting the collagen-binding domain of DMP1 to the hydrophilic C-terminal of amelogenin. Fluorescein isothiocyanate-coupled oligopeptide was applied to the completely demineralized dentine collagen and examined using fluorescent microscopy. The nucleation and growth of hydroxyapatite were initiated by immersing oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Scanning electron microscopy (SEM), transmission electron microscopy, and selected area electron diffraction (SAED) were used to examine the formation. Dentine slices were acid-etched, coated with oligopeptide, and immersed into a metastable calcium phosphate solution. Dentine mineralization was evaluated by SEM, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR).


Fluorescent dentine collagen was identified in the specimens. The nucleation and growth of crystals were detected after immersing the oligopeptide into calcium chloride and sodium hydrogen phosphate solutions. Under SEM, crystals were observed covering the oligopeptide-coated dentine surface, within the demineralized dentine collagen matrix and occluding dentinal tubules. SAED, XRD, and FTIR confirmed that the crystals were hydroxyapatite.


A novel oligopeptide-simulating DMP1 was developed, that can bind to collagen fibrils, initiate mineralization, and induce biomimetic mineralization of dentine.

Clinical relevance

Biomimetic mineralization of dentine facilitated by this oligopeptide is a potential therapeutic technique for the management of dentine hypersensitivity.


Biomineralization Dentine matrix protein Oligopeptide Biomimetic remineralization Collagen Hydroxyapatite 



This study was supported by grants from the NSFC/RGC Joint Research Scheme (N_HKU 776/10 and no. 81061160511), NSFC (no. 30973352), Outstanding Youth Fund of the Board of Education of Anhui Province (no. 2011SQRL063), and Youth Foundation of Anhui Provincial Natural Science Foundation (no. 1208085QH144).

Conflict of interest

The authors declare that they have no conflicts of interest and/or any financial interests in any of the used products.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Faculty of DentistryThe University of Hong KongHong KongChina
  2. 2.College of StomatologyAnhui Medical UniversityHefeiPeople’s Republic of China

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