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Clinical Oral Investigations

, Volume 18, Issue 3, pp 901–908 | Cite as

Induction of IL-6 and MMP-8 in human periodontal fibroblasts by static tensile strain

  • Collin JacobsEmail author
  • Christian Walter
  • Thomas Ziebart
  • Sarah Grimm
  • Dan Meila
  • Elena Krieger
  • Heinrich Wehrbein
Original Article

Abstract

Objectives

Mechanical loading is a potential activator of inflammation and able to stimulate factors for periodontal and alveolar bone destruction. Aim of this study was to investigate the inflammatory response and synthesis of proteinases by human periodontal ligament fibroblast (HPdLF) dependent on different strengths of static tensile strain (STS).

Materials and methods

HPdLFs were loaded with different STS strengths (1, 5, and 10 %) in vitro. Gene expressions of cyclooxygenase (COX)-2 and interleukin (IL)-6 were analyzed by quantitative real-time polymerase chain reaction. Production of IL-6, prostaglandin E2 (PGE2), matrix metalloproteinase (MMP)-8, and tissue inhibitors of matrix metalloproteinase (TIMP)-1 were measured by enzyme-linked immunosorbent assay. Receptor activator of nuclear factor-kappa ligand (RANKL) synthesis was detected by immunocytochemical staining.

Results

Ten percent STS led to an increased gene expression of IL-6 and COX-2 (34.4-fold) in HPdLF, and 1 and 5 % STS slightly reduced the gene expression of IL-6. Synthesis of IL-6 was significantly reduced by 1 % STS and stimulated by 10 % STS. Ten percent STS significantly induced PGE2 production. RANKL was not detectable at any strength of STS. MMP-8 synthesis showed significantly higher values only at 10 % STS, but TIMP-1 was stimulated by 5 and 10 % STS, resulting into highest TIMP-1/MMP-8 ratio at 5 % STS.

Conclusions

High-strength STS is a potent inducer of periodontal inflammation and MMP-8, whereas low-strength STS shows an anti-inflammatory effect. Moderate-strength STS causes the highest TIMP-1/MMP-8 ratio, leading to appropriate conditions for reformation of the extracellular matrix.

Clinical relevance

Furthermore, this study points out that the strength of force plays a pivotal role to achieve orthodontic tooth movement without inducing periodontal inflammation and to activate extracellular matrix regeneration.

Keywords

Orthodontic tooth movement Aseptic inflammation Static strain Parodontitis 

Notes

Acknowledgments

We thank Dr. Jutta Goldschmidt, Ute Zerfass, and Lotte Groothusen for their assistance in the laboratory and Katherine Taylor for orthographic correction of the article.

Conflict of interest

The authors declare that no competing financial interest exists.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Collin Jacobs
    • 1
    Email author
  • Christian Walter
    • 2
  • Thomas Ziebart
    • 2
  • Sarah Grimm
    • 1
  • Dan Meila
    • 3
  • Elena Krieger
    • 1
  • Heinrich Wehrbein
    • 1
  1. 1.Department of Orthodontics, University Medical CenterJohannes Gutenberg University MainzMainzGermany
  2. 2.Department of Oral and Maxillofacial Surgery, Medical CenterJohannes Gutenberg University MainzMainzGermany
  3. 3.Department of Diagnostic and Interventional NeuroradiologyHannover Medical SchoolHannoverGermany

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