Clinical Oral Investigations

, Volume 17, Issue 4, pp 1235–1242 | Cite as

Influence of IL-6 haplotypes on clinical and inflammatory response in aggressive periodontitis

  • L. Nibali
  • G. Pelekos
  • F. D’Aiuto
  • N. Chaudhary
  • R. Habeeb
  • D. Ready
  • M. Parkar
  • N. Donos
Original Article



The aim of this study was to investigate the inflammatory response in aggressive periodontitis (AgP) patients after periodontal therapy and associate these changes to subjects’ interleukin-6 (IL-6) genetic variants.

Materials and methods

Twelve non-smoking UK Caucasian patients with AgP were selected based on their IL6 haplotypes (six haplotype positive and six haplotype negative based on polymorphisms rs 2069827 and rs 2069825) and underwent full mouth non-surgical periodontal therapy, followed by open flap surgery. Gingival crevicular fluid (GCF) and peripheral blood samples were taken at baseline and at six different time points after treatment. Gingival biopsy samples were harvested during surgery and underwent immunohistochemical analysis for identification of IL-6.


An overall improvement in clinical periodontal parameters was observed following periodontal therapy. Haplotype status was associated with clinical presentation, Aggregatibacter actinomycetemcomitans counts in subgingival plaque samples, white cell count, neutrophils, red cell count and haemoglobin. GCF IL-6 concentrations increased dramatically 1 day after surgery and IL-6 haplotype-positive subjects exhibited a higher magnitude in this increase.


IL6 haplotypes may have an effect on clinical presentation and magnitude and kinetics of local and systemic inflammatory responses following non-surgical and surgical periodontal therapy in aggressive periodontitis.

Clinical relevance

Detecting IL-6 haplotype-positive periodontitis patients might become helpful in identifying subjects prone to excessive inflammatory response and increased periodontal breakdown.


Periodontitis Genetic Inflammation Interleukin 



This study was supported by the Periodontal Research Fund of the UCL Eastman Dental Institute, and it was undertaken at UCL, which received a proportion of funding from the Department of Health’s National Institute of Health Research (NIHR) Biomedical Research Centres funding scheme. FDA holds a Clinical Senior Lectureship Award supported by the UK Clinical Research Collaboration. The help of Dr. Ulpee Darbar for patient recruitment and of Dr. Nicola Mordan for the histological analysis is gratefully acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • L. Nibali
    • 1
  • G. Pelekos
    • 1
  • F. D’Aiuto
    • 1
  • N. Chaudhary
    • 1
  • R. Habeeb
    • 1
  • D. Ready
    • 2
  • M. Parkar
    • 1
  • N. Donos
    • 1
    • 3
  1. 1.Periodontology Unit and Division of Clinical Research, Eastman Dental Institute and HospitalUniversity College London (UCL)LondonUK
  2. 2.Eastman Dental HospitalUCLH (University College London Hospital) NHS (National Health Service) Foundation TrustLondonUK
  3. 3.Periodontology UnitUCL Eastman Dental InstituteLondonUK

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