Clinical Oral Investigations

, Volume 17, Issue 1, pp 87–95 | Cite as

Clinical and microbiological evaluation of high intensity diode laser adjutant to non-surgical periodontal treatment: a 6-month clinical trial

  • Vanessa Tubero Euzebio Alves
  • Ana Karina Pinto de Andrade
  • Janaita Maria Toaliar
  • Marina Clemente Conde
  • Denise Maria Zezell
  • Silvana Cai
  • Claudio Mendes Pannuti
  • Giorgio De Micheli
Original Article



This randomized split-mouth clinical trial was designed to evaluate the efficacy of scaling and root planing associated to the high-intensity diode laser on periodontal therapy by means of clinical parameters and microbial reduction.

Materials and methods

A total of 36 chronic periodontitis subjects, of both genders, were selected. One pair of contralateral single-rooted teeth with pocket depth >5 mm was chosen from each subject. All patients received non-surgical periodontal treatment, after which the experimental teeth were designated to either test or control groups. Both teeth received scaling, root planing and coronal polishing (SRP) and teeth assigned to the test group (SRP + DL) were irradiated with the 808 ± 5 nm diode laser, for 20 s, in two isolated appointments, 1 week apart. The laser was used in the continuous mode, with 1.5 W and power density of 1,193.7 W/cm2. Clinical and microbiological data were collected at baseline, 6 weeks and 6 months after therapy.


There was a significant improvement of all the clinical parameters—clinical attachment level (CAL), probing depth (PD), plaque index (PI) and Bleeding on Probing (BOP)—for both groups (P < 0.001), with no statistical difference between them at the 6 weeks and the 6 months examinations. As for microbiological analysis, a significant reduction after 6 weeks (P > 0.05) was observed as far as colony forming units (CFU) is concerned, for both groups. As for black-pigmented bacteria, a significant reduction was observed in both groups after 6 months. However, the difference between test and control groups was not significant. There was no association between group and presence of Porphyromonas gingivalis, Prevotella intermedia and Aggregatibacter actinomycetemcomitans at any time of the study.


After 6 months of evaluation, the high-intensity diode laser has not shown any additional benefits to the conventional periodontal treatment.

Clinical relevance

The high intensity diode laser did not provide additional benefits to non-surgical periodontal treatment. More studies are necessary to prove the actual need of this type of laser in the periodontal clinical practice.


Bacterial reduction Chronic periodontitis High power diode laser Non-surgical periodontal treatment 



The authors would like to thank São Paulo Research Foundation—FAPESP, research grants 2005/56965–8. Research grants: São Paulo Research Foundation (FAPESP, São Paulo, SP, Brazil) # 05/5695-8 and Coordination for the Improvement of Higher Educated Personnel (CAPES, Brasilia, DF, Brazil).

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Vanessa Tubero Euzebio Alves
    • 1
  • Ana Karina Pinto de Andrade
    • 1
  • Janaita Maria Toaliar
    • 1
  • Marina Clemente Conde
    • 1
  • Denise Maria Zezell
    • 2
  • Silvana Cai
    • 3
  • Claudio Mendes Pannuti
    • 1
  • Giorgio De Micheli
    • 1
  1. 1.Department of Stomatology, Discipline of PeriodonticsSchool of Dentistry at the University of São Paulo (USP)São PauloBrazil
  2. 2.Laboratory of Biophotonics, Center for Lasers and Applications, IPEN—CNEN/SPSão PauloBrazil
  3. 3.Microbiology Department of the Institute of Biomedical Sciences at the University of São Paulo (USP)São PauloBrazil

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