Clinical Oral Investigations

, Volume 16, Issue 2, pp 581–590 | Cite as

Beta-tricalcium phosphate/type I collagen cones with or without a barrier membrane in human extraction socket healing: clinical, histologic, histomorphometric, and immunohistochemical evaluation

  • Bozidar M. B. Brkovic
  • Hari S. Prasad
  • Michael D. Rohrer
  • George Konandreas
  • George Agrogiannis
  • Dragana Antunovic
  • George K. B. Sándor
Original Article

Abstract

The aim of this study was to investigate the healing of human extraction sockets filled with β-tricalcium phosphate and type I collagen (β-TCP/Clg) cones with or without a barrier membrane. Twenty patients were divided in two groups: (A) β-TCP/Clg non-membrane and (B) β-TCP/Clg + barrier membrane. Clinical examination and biopsies from the grafted sites were collected 9 months later. Bone samples were analyzed using histomorphometry and immunohistochemistry. The horizontal dimension of the alveolar ridge was significantly reduced 9 months after socket preservation in the non-membrane group. There was bone formation with no significant differences between the two groups in the areas occupied by new bone (A = 42.4%; B = 45.3%), marrow (A = 42.7%; B = 35.7%), or residual graft (A = 9.7%; B = 12.5%). Immunohistochemistry revealed osteonectin expression in both groups. Both groups demonstrated sufficient amounts of vital bone and socket morphology to support dental implant placement after the 9-month healing period. A future trial to evaluate the alveolar outcomes at an earlier 6-month time point rather than the 9 months used in this study would be of interest.

Keywords

Socket preservation Beta-tricalcium phosphate Collagen type I Barrier membrane Histomorphometry Immunohistochemistry 

Notes

Acknowledgments

This study was supported by Septodont, France, Grant No. 2207-2006.

Conflicts of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Bozidar M. B. Brkovic
    • 1
  • Hari S. Prasad
    • 2
  • Michael D. Rohrer
    • 2
    • 3
  • George Konandreas
    • 4
  • George Agrogiannis
    • 5
  • Dragana Antunovic
    • 6
  • George K. B. Sándor
    • 7
    • 8
  1. 1.Clinic of Oral Surgery, Faculty of DentistryUniversity of BelgradeBelgradeSerbia
  2. 2.Hard Tissue Research Laboratory, School of DentistryUniversity of MinnesotaMinneapolisUSA
  3. 3.Division of Oral Pathology, School of DentistryUniversity of MinnesotaMinneapolisUSA
  4. 4.AthensGreece
  5. 5.Department of Pathology, School of MedicineNational and Kapodistrian University of AthensAthensGreece
  6. 6.BelgradeSerbia
  7. 7.Tissue Engineering, Regea Institute for Regenerative MedicineUniversity of TampereTampereFinland
  8. 8.Department of Oral and Maxillofacial SurgeryUniversity of OuluOuluFinland

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