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Clinical Oral Investigations

, Volume 14, Issue 1, pp 19–25 | Cite as

The evaluation of a novel method comparing quantitative light-induced fluorescence (QLF) with spectrophotometry to assess staining and bleaching of teeth

  • A. A. AdeyemiEmail author
  • F. D. Jarad
  • E. de Josselin de Jong
  • N. Pender
  • S. M. Higham
Original Article

Abstract

This study reports the development and evaluation of a novel method using quantitative light-induced fluorescence (QLF), which enables its use for quantifying and assessing whole tooth surface staining and tooth whitening. The method was compared with a spectrophotometer to assess reliability. Two experimental phases, intrinsic stain formation and tooth whitening, were conducted in vitro on 16 extracted bovine teeth. Intrinsic stains were developed via access through lingual surfaces and root canals of these teeth using tea solution (2 g/100 ml, Marks and Spencer Extra Strong Tea, Marks and Spencer, London, UK) for 6 days. Stains were removed using 33% hydrogen peroxide (VWR Prolab, Leicestershire, UK) in cycles over 150 min. Stain development/whitening was monitored with QLF (Inspektor Research systems, Amsterdam, Netherlands) and spectrophotometry (Easy shade, Vita Zahnfabrik, Bad Säckingen, Germany). Parameters Delta F for QLF and Delta E for the spectrophotometer were obtained. The progression of stain intensity and removal observed by the methods were tested for correlation using Pearson's correlation coefficient. Intra-examiner reliability for each method was tested. QLF showed a high correlation with spectrophotometry for detecting and monitoring intrinsic tooth stain progression (Pearson coefficient r was −0.987 with correlation significant p < 0.0001). For stain removal, the Pearson coefficient (r) between both methods was −0.906 with no significance p = 0.094. The use of an external reference material in combination with the inner patch QLF analysis technique had the ability to detect and measure whole tooth surface staining and its removal longitudinally. The reliability of the method shows a potential clinical application.

Keywords

QLF Fluorescence Spectrophotometry Staining Tooth whitening 

Notes

Acknowledgement

The authors would like to thank Mr Lee Cooper for his technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Adeyemi AA, Jarad FD, Pender N et al (2006) Comparison of quantitative light-induced fluorescence (QLF) and digital imaging applied for the detection and quantification of staining and stain removal on teeth. J Dent 34:460–466CrossRefPubMedGoogle Scholar
  2. 2.
    Amaechi BT, Higham SM (2002) Development of a quantitative method to monitor the effect of a tooth whitening agent. J Clin Dent 131:100–103Google Scholar
  3. 3.
    Bentley C, Leonard RH, Nelson CF et al (1999) Quantification of vital bleaching by computer analysis of photographic images. J Am Dent Assoc 130:809–816PubMedGoogle Scholar
  4. 4.
    Berman LH (1982) Intrinsic staining and hypoplastic enamel: etiology and treatment alternative. Gen Dent 30:484–488PubMedGoogle Scholar
  5. 5.
    CIE publication (1986) CIE Colomerimetry, 2nd edn. Central Bureau of the CIE, Paris, No 15. 2Google Scholar
  6. 6.
    Clarke FJ (1983) Measurement of color of human teeth in dental ceramics. In: McLean JW (ed) Proceedings of the first international symposium on ceramics. Quintessence, ChicagoGoogle Scholar
  7. 7.
    Culpepper WD (1970) A comparative study of shade matching procedures. J Prosthet Dent 24:166–173CrossRefPubMedGoogle Scholar
  8. 8.
    Derdilopoulou FV, Zantner C, Neumann K et al (2007) Evaluation of visual and spectrophotometric shade analyses: a clinical comparison of 3758 teeth. Int J Prosthodont 20:414–416PubMedGoogle Scholar
  9. 9.
    Donahue JL, Goodkind RJ, Schwabacher WB et al (1991) Shade colour discrimination by men and women. J Prosthet Dent 65:699–703CrossRefPubMedGoogle Scholar
  10. 10.
    Jarad FD, Russell MD, Moss BW (2005) The use of digital imaging for colour matching and communication in restorative dentistry. Br Dent J 199:43–49CrossRefPubMedGoogle Scholar
  11. 11.
    Joiner A, Pickles MJ, Matheson JR et al (2002) Whitening toothpastes: effects on tooth stain and enamel. Int Dent J 52:424–430Google Scholar
  12. 12.
    Joiner A (2004) Tooth colour: a review of the literature. J Dent 32:3–12CrossRefPubMedGoogle Scholar
  13. 13.
    Joiner A (2006) The bleaching of teeth: a review of the literature. J Dent 34:412–419CrossRefPubMedGoogle Scholar
  14. 14.
    Macentee M, Lakowski R (1981) Instrumental color measurement of vital and extracted human teeth. J Oral Rehabil 8:203–208CrossRefPubMedGoogle Scholar
  15. 15.
    Myers ML, Dickson GL, Curtis JW et al (1995) Evaluating color change following vital tooth bleaching. J Esthet Dent 7:256–262CrossRefPubMedGoogle Scholar
  16. 16.
    Okubo SR, Kanawati A, Richards MW et al (1998) Evaluation of visual and instrument shade matching. J Prosthet Dent 80:642–648CrossRefPubMedGoogle Scholar
  17. 17.
    Paul S, Peter A, Pietrobon N et al (2002) Visual and spectrophotometric shade analysis of human teeth. J Dent Res 81:578–582CrossRefPubMedGoogle Scholar
  18. 18.
    Pretty IA, Edgar WM, Higham SM (2001) The use of QLF to quantify in vitro whitening in a product testing model. Br Dent J 191:566–569CrossRefPubMedGoogle Scholar
  19. 19.
    Pretty IA, Hall AF, Smith PW et al (2002) The intra- and inter-examiner reliability of quantitative light-induced fluorescence (QLF) analyses. Br Dent J 193:105–109CrossRefPubMedGoogle Scholar
  20. 20.
    Romane D, Bendika Z, Senakola E et al (2005) The effect of video repositioning on the reliability of light-induced fluorescence imaging: an in vivo study. Caries Res 39:397–402CrossRefPubMedGoogle Scholar
  21. 21.
    Sarrett DC (2002) Whitening today. J Am Dent Assoc 133:1535–1538PubMedGoogle Scholar
  22. 22.
    Sulieman M, Addy M, Rees JS (2003) Development and evaluation of a method in vitro to study the effectiveness of tooth bleaching. J Dent 31:415–422CrossRefPubMedGoogle Scholar
  23. 23.
    Van der Burgt TP, Ten Bosch JJ, Borsboom PCF et al (1985) A new method for matching tooth colors with color standards. J Dent Res 64:837–841PubMedGoogle Scholar
  24. 24.
    Watts A, Addy M (2001) Tooth discolouration and staining a review of the literature. Br Dental J 190:309–316CrossRefGoogle Scholar
  25. 25.
    Zantner C, Derdilopoulou F, Martus P et al (2006) Randomized clinical trial on the efficacy of 2 over-the-counter whitening systems. Quintessence Int 37:695–706PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • A. A. Adeyemi
    • 1
    Email author
  • F. D. Jarad
    • 1
  • E. de Josselin de Jong
    • 1
    • 2
  • N. Pender
    • 1
  • S. M. Higham
    • 1
  1. 1.School of Dental SciencesThe University of LiverpoolLiverpoolUK
  2. 2.Inspektor Research SystemsAmsterdamThe Netherlands

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