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Clinical Oral Investigations

, Volume 8, Issue 4, pp 233–237 | Cite as

Levels of parotid and submandibular/sublingual salivary immunoglobulin A in response to experimental gingivitis in humans

  • R. SeemannEmail author
  • S. J. Hägewald
  • V. Sztankay
  • J. Drews
  • M. Bizhang
  • A. Kage
Original article

Abstract

Salivary secretory IgA (s-IgA) is considered to act as an important first line of defense mechanism in the oral cavity. It has therefore been suggested that an increased antigenic load would induce an increase in salivary IgA production. This study investigated the pure glandular levels of salivary IgA in parotid and submandibular/sublingual (SM/SL) saliva during plaque accumulation leading to experimental gingivitis. Starting from regular oral hygiene, 14 healthy, nonsmoking men refrained from all oral hygiene measures for 12 days. On days −2, 0, 3, 6, and 12 a plaque index, a bleeding index, and unstimulated and stimulated saliva from the parotid and the SM/SL glands were measured. Salivary IgA was quantified using a sandwich ELISA. All subjects developed gingivitis as measured by a bleeding index. Compared to baseline the salivary flow rate was increased on day 12. Regarding the secretion rate of IgA there was a statistically significant increase in stimulated parotid saliva but not SM/SL saliva compared to baseline after 6 and 12 days without oral hygiene. No significant changes were observed for the concentration of IgA during the trial. Thus, in healthy subjects with regular oral hygiene the development of plaque induced gingivitis is associated with increased salivary gland output and increased total IgA output levels in stimulated parotid saliva but not in SM/SL saliva.

Keywords

Salivary IgA Experimental gingivitis Pure glandular saliva Plaque Antigen load 

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • R. Seemann
    • 1
    Email author
  • S. J. Hägewald
    • 2
  • V. Sztankay
    • 1
  • J. Drews
    • 1
  • M. Bizhang
    • 1
  • A. Kage
    • 3
  1. 1.Department of Operative and Preventive Dentistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany
  2. 2.Department of Periodontology and Synoptic Dentistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany
  3. 3.Institute of Laboratory Medicine and Pathobiochemistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany

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