Clinical Oral Investigations

, Volume 8, Issue 4, pp 233–237 | Cite as

Levels of parotid and submandibular/sublingual salivary immunoglobulin A in response to experimental gingivitis in humans

  • R. SeemannEmail author
  • S. J. Hägewald
  • V. Sztankay
  • J. Drews
  • M. Bizhang
  • A. Kage
Original article


Salivary secretory IgA (s-IgA) is considered to act as an important first line of defense mechanism in the oral cavity. It has therefore been suggested that an increased antigenic load would induce an increase in salivary IgA production. This study investigated the pure glandular levels of salivary IgA in parotid and submandibular/sublingual (SM/SL) saliva during plaque accumulation leading to experimental gingivitis. Starting from regular oral hygiene, 14 healthy, nonsmoking men refrained from all oral hygiene measures for 12 days. On days −2, 0, 3, 6, and 12 a plaque index, a bleeding index, and unstimulated and stimulated saliva from the parotid and the SM/SL glands were measured. Salivary IgA was quantified using a sandwich ELISA. All subjects developed gingivitis as measured by a bleeding index. Compared to baseline the salivary flow rate was increased on day 12. Regarding the secretion rate of IgA there was a statistically significant increase in stimulated parotid saliva but not SM/SL saliva compared to baseline after 6 and 12 days without oral hygiene. No significant changes were observed for the concentration of IgA during the trial. Thus, in healthy subjects with regular oral hygiene the development of plaque induced gingivitis is associated with increased salivary gland output and increased total IgA output levels in stimulated parotid saliva but not in SM/SL saliva.


Salivary IgA Experimental gingivitis Pure glandular saliva Plaque Antigen load 


  1. 1.
    Brandtzaeg P (1971) Human secretory immunoglobulins. VII. Concentrations of parotid IgA and other secretory proteins in relation to the rate of flow and duration of secretory stimulus. Arch Oral Biol 16:1295–1310CrossRefPubMedGoogle Scholar
  2. 2.
    Brandtzaeg P (1989) Salivary immunoglobulins. In: Tenovuo J (ed) Human saliva: clinical chemistry and microbiology. CRC, Boca Raton, pp 2–54Google Scholar
  3. 3.
    Dimitriou L, Sharp NC, Doherty M (2002) Circadian effects on the acute responses of salivary cortisol and IgA in well trained swimmers. Br J Sports Med 36:260–264CrossRefPubMedGoogle Scholar
  4. 4.
    Emmings FG, Evans RT, Genco RJ (1975) Antibody response in the parotid fluid and serum of irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans. Infect Immun 12:281–292PubMedGoogle Scholar
  5. 5.
    Garrett JR, Proctor GB (1998) Control of salivation. In: Linden RWA (ed) The scientific basis of eating. Frontal oral biology. Karger, Basel, pp 135–155Google Scholar
  6. 6.
    Gomez E, Ortiz V, Saint-Martin B, Boeck L, Diaz-Sanchez V, Bourges H (1993) Hormonal regulation of the secretory IgA (sIgA) system: estradiol- and progesterone-induced changes in sIgA in parotid saliva along the menstrual cycle. Am J Reprod Immunol 29:219–223PubMedGoogle Scholar
  7. 7.
    Hagewald S, Bernimoulin JP, Kottgen E, Kage A (2000) Total IgA and Porphyromonas gingivalis-reactive IgA in the saliva of patients with generalised early-onset periodontitis. Eur J Oral Sci 108:147–153CrossRefPubMedGoogle Scholar
  8. 8.
    Harding J, Berry WC, Marsh C, Jolliff CR (1980) Salivary antibodies in acute gingivitis. J Periodontol 51:63–69PubMedGoogle Scholar
  9. 9.
    Henskens YM, van der Weijden FA, van den Keijbus PA, Veerman EC, Timmerman MF, van der Velden U, Amerongen AV (1996) Effect of periodontal treatment on the protein composition of whole and parotid saliva. J Periodontol 67:205–212PubMedGoogle Scholar
  10. 10.
    Jalil RA, Ashley FP, Wilson RF (1992) The relationship between 48-h dental plaque accumulation in young human adults and the concentrations of hypothiocyanite, ‘free’ and ‘total’ lysozyme, lactoferrin and secretory immunoglobulin A in saliva. Arch Oral Biol 37:23–28CrossRefPubMedGoogle Scholar
  11. 11.
    Jalil RA, Ashley FP, Wilson RF, Wagaiyu EG (1993) Concentrations of thiocyanate, hypothiocyanite, ‘free’ and ‘total’ lysozyme, lactoferrin and secretory IgA in resting and stimulated whole saliva of children aged 12–14 years and the relationship with plaque accumulation and gingivitis. J Periodontal Res 28:130–136PubMedGoogle Scholar
  12. 12.
    Kalk WW, Vissink A, Spijkervet FK, Bootsma H, Kallenberg CG, Nieuw Amerongen AV (2001) Sialometry and sialochemistry: diagnostic tools for Sjogren’s syndrome. Ann Rheum Dis 60:1110–1116CrossRefPubMedGoogle Scholar
  13. 13.
    Kalk WW, Vissink A, Stegenga B, Bootsma H, Nieuw Amerongen AV, Kallenberg CG (2002) Sialometry and sialochemistry: a non-invasive approach for diagnosing Sjogren’s syndrome. Ann Rheum Dis 61:137–144CrossRefPubMedGoogle Scholar
  14. 14.
    Kilian M, Mestecky J, Russell MW (1988) Defense mechanisms involving Fc-dependent functions of immunoglobulin A and their subversion by bacterial immunoglobulin A proteases. Microbiol Rev 52:296–303PubMedGoogle Scholar
  15. 15.
    Kinane DF, Lappin DF (2001) Clinical, pathological and immunological aspects of periodontal disease. Acta Odontol Scand 59:154–160CrossRefPubMedGoogle Scholar
  16. 16.
    Korsrud FR, Brandtzaeg P (1980) Quantitative immunohistochemistry of immunoglobulin- and J-chain-producing cells in human parotid and submandibular salivary glands. Immunology 39:129–140PubMedGoogle Scholar
  17. 17.
    Lashley KS (1916) Reflex secretion of the human parotid gland. J Exp Psychol 16:461–493Google Scholar
  18. 18.
    Lie MA, Myint MM, Schenck K, Timmerman MF, van der Velden U, van der Weijden GA, Loos BG (2002) Parotid salivary S-IgA antibodies during experimental gingivitis in smokers and non-smokers. J Periodontal Res 37:86–92CrossRefPubMedGoogle Scholar
  19. 19.
    Lindstrom FD, Folke LE (1973) Salivary IgA in periodontal disease. Acta Odontol Scand 31:31–34PubMedGoogle Scholar
  20. 20.
    Löe H, Theilade E, Jensen SB (1965) Experimental gingivitis in man. J Periodontol 36:177–187PubMedGoogle Scholar
  21. 21.
    Macpherson AJ, Gatto D, Sainsbury E, Harriman GR, Hengartner H, Zinkernagel RM (2000) A primitive T cell-independent mechanism of intestinal mucosal IgA responses to commensal bacteria. Science 288:2222–2226CrossRefPubMedGoogle Scholar
  22. 22.
    Macpherson AJ, Lamarre A, McCoy K, Harriman GR, Odermatt B, Dougan G, Hengartner H, Zinkernagel RM (2001) IgA production without mu or delta chain expression in developing B cells. Nat Immunol 2:625–631CrossRefPubMedGoogle Scholar
  23. 23.
    Marcotte H, Lavoie MC (1998) Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 62:71–109Google Scholar
  24. 24.
    McGhee JR, Kiyono H (1994) Effective mucosal immunity. Current concepts for vaccine delivery and immune response analysis. Int J Technol Assess Health Care 10:93–106PubMedGoogle Scholar
  25. 25.
    McGhee JR, Mestecky J, Dertzbaugh MT, Eldridge JH, Hirasawa M, Kiyono H (1992) The mucosal immune system: from fundamental concepts to vaccine development. Vaccine 10:75–88CrossRefPubMedGoogle Scholar
  26. 26.
    McNabb PC, Tomasi TB (1981) Host defense mechanisms at mucosal surfaces. Annu Rev Microbiol 35:477–496CrossRefPubMedGoogle Scholar
  27. 27.
    Michalek SM, Katz J, Childers NK (2001) A vaccine against dental caries: an overview. BioDrugs 15:501–508PubMedGoogle Scholar
  28. 28.
    Myint MM, Steinsvoll S, Odden K, Dobloug J, Schenck K (1997) Salivary IgA responses to bacteria in dental plaque as related to periodontal and HIV infection status. Eur J Oral Sci 105:562–570Google Scholar
  29. 29.
    Nair PN, Schroeder HE (1983) Local immune response to repeated topical antigen application in the simian labial mucosa. Infect Immun 41:399–409PubMedGoogle Scholar
  30. 30.
    Nair PN, Schroeder HE (1983) Retrograde access of antigens to the minor salivary glands in the monkey Macaca fascicularis. Arch Oral Biol 28:145–152CrossRefPubMedGoogle Scholar
  31. 31.
    Oon CH, Lee J (1972) A controlled quantitative study of parotid salivary secretory IgA-globulin in normal adults. J Immunol Methods 2:45–48CrossRefPubMedGoogle Scholar
  32. 32.
    Orstavik D, Brandtzaeg P (1975) Secretion of parotid IgA in relation to gingival inflammation and dental caries experience in man. Arch Oral Biol 20:701–704CrossRefPubMedGoogle Scholar
  33. 33.
    Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE (2001) Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783CrossRefPubMedGoogle Scholar
  34. 34.
    Quigley GA, Hein JW (1962) Comparative cleansing efficiency of manual and power brushing. J Am Dent Assoc 65:26PubMedGoogle Scholar
  35. 35.
    Ranney RR, Ruddy S, Tew JG, Welshimer HJ, Palcanis KG, Segreti A (1981) Immunological studies of young adults with severe periodontitis. I. Medical evaluation and humoral factors. J Periodontal Res 16:390–402PubMedGoogle Scholar
  36. 36.
    Roitt IM, Lehner T (1983) Immune responses to dental bacterial plaque. In: Roitt IM, Lehner T (eds) Immunology of oral diseases. Blackwell Scientific, London, pp 305–323Google Scholar
  37. 37.
    Rudney JD, Krig MA, Neuvar EK, Soberay AH, Iverson L (1991) Antimicrobial proteins in human unstimulated whole saliva in relation to each other, and to measures of health status, dental plaque accumulation and composition. Arch Oral Biol 36:497–506CrossRefPubMedGoogle Scholar
  38. 38.
    Sandholm L, Gronblad E (1984) Salivary immunoglobulins in patients with juvenile periodontitis and their healthy siblings. J Periodontol 55:9–12PubMedGoogle Scholar
  39. 39.
    Saxer UP, Muhlemann HR (1975) Motivation and education. SSO Schweiz Monatsschr Zahnheilkd 85:905–919PubMedGoogle Scholar
  40. 40.
    Schenck K, Poppelsdorf D, Denis C, Tollefsen T (1993) Levels of salivary IgA antibodies reactive with bacteria from dental plaque are associated with susceptibility to experimental gingivitis. J Clin Periodontol 20:411–417PubMedGoogle Scholar
  41. 41.
    Stuchell RN, Mandel ID (1978) Studies of secretory IgA in caries-resistant and caries-susceptible adults. Adv Exp Med Biol 107:341–348PubMedGoogle Scholar
  42. 42.
    Talham GL, Jiang HQ, Bos NA, Cebra JJ (1999) Segmented filamentous bacteria are potent stimuli of a physiologically normal state of the murine gut mucosal immune system. Infect Immun 67:1992–2000PubMedGoogle Scholar
  43. 43.
    Van der Velden U, Abbas F, Winkel EG (1986) Probing considerations in relation to susceptibility to periodontal breakdown. J Clin Periodontol 13:894–899PubMedGoogle Scholar
  44. 44.
    Walker J (1981) Antibody responses of monkeys to oral and local immunization with Streptococcus mutans. Infect Immun 31:61–70PubMedGoogle Scholar
  45. 45.
    Wolf RO (1964) Regulated vacuum system for collecting submaxillary and sublingual saliva. J Dent Res 43:303–306PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • R. Seemann
    • 1
    Email author
  • S. J. Hägewald
    • 2
  • V. Sztankay
    • 1
  • J. Drews
    • 1
  • M. Bizhang
    • 1
  • A. Kage
    • 3
  1. 1.Department of Operative and Preventive Dentistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany
  2. 2.Department of Periodontology and Synoptic Dentistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany
  3. 3.Institute of Laboratory Medicine and Pathobiochemistry, Virchow CampusCharité University Medical School of BerlinBerlinGermany

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