Journal of Orthopaedic Science

, Volume 15, Issue 2, pp 240–244 | Cite as

Effect of low-intensity pulsed ultrasound on fracture callus mineral density and flexural strength in rabbit tibial fresh fracture

  • Kazem Shakouri
  • Bina Eftekharsadat
  • Mohammad Reza Oskuie
  • Jafar Soleimanpour
  • Mohammad Kazem Tarzamni
  • Yagoub Salekzamani
  • Yousef Hoshyar
  • Nariman Nezami
Original Article



Low-intensity ultrasound is a biophysical intervention on a fracture repair process. However, the effect of low-intensity ultrasound therapy on fracture healing is controversial. The aim of the present study was to evaluate the effect of low-intensity pulsed ultrasound (LIPUS) therapy on the fracture healing process, including mineral density and strength of callus using a rabbit model.


A total of 30 rabbits underwent unilateral, transverse, and mid-tibia open osteotomies that were stabilized with external fixators. Then, the animals were divided into two study groups composed of 15 rabbits each: the case group (US), which were exposed to low-intensity pulsed ultrasound with 30 mW/cm2 intensity and 1.5 MHz sine waves; and the control group (C), which underwent sham ultrasound treatment. Callus development and mineral density were evaluated using multidetector computed tomography at 2, 5, and 8 weeks, after which the animals were killed. Three-point bending tests of both healed and intact bones were assessed and compared.


The results demonstrated that the callus mineral density in the US group was higher than in the C group (1202.20 ± 81.30 vs. 940.66 ± 151.58 HU; P = 0.001) at the end of the 8th week. The mean recorded three-point bending test score of healed bones in the US group was not significantly different from that of the C group (359.35 ± 173.39 vs. 311.02 ± 80.58 N; P = 0.114).


The present study showed that low-intensity pulsed ultrasound enhanced callus mineral density with an insignificant increase in the strength of the fractured bone.


Fracture Healing Distraction Osteogenesis Pulse Ultrasound Intact Bone Early Weight Bearing 
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Copyright information

© The Japanese Orthopaedic Association 2010

Authors and Affiliations

  • Kazem Shakouri
    • 1
  • Bina Eftekharsadat
    • 1
  • Mohammad Reza Oskuie
    • 1
  • Jafar Soleimanpour
    • 2
  • Mohammad Kazem Tarzamni
    • 3
  • Yagoub Salekzamani
    • 1
  • Yousef Hoshyar
    • 1
  • Nariman Nezami
    • 2
    • 3
    • 4
    • 5
    • 6
  1. 1.Department of Physical Medicine and RehabilitationTabriz University (Medical Sciences)TabrizIran
  2. 2.Department of Orthopedic SurgeryTabriz University (Medical Sciences)TabrizIran
  3. 3.Department of RadiologyTabriz University (Medical Sciences)TabrizIran
  4. 4.Drug Applied Research CenterTabriz University (Medical Sciences)TabrizIran
  5. 5.Young Researchers ClubTabriz Islamic Azad UniversityTabrizIran
  6. 6.Clinical Pharmacy Laboratory, Drug Applied Research CenterTabriz University (Medical Sciences)TabrizIran

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