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The interplay between copper(II), human serum albumin, fatty acids, and carbonylating agent interferes with Cys 34 thiol reactivity and copper binding

  • Ana Z. PenezićEmail author
  • Jelena M. Aćimović
  • Ivan D. Pavićević
  • Vesna B. Jovanović
  • Marija Takić
  • Ljuba M. Mandić
Original Paper
  • 57 Downloads

Abstract

Cys34 thiol group of human serum albumin (HSA) represents major plasma antioxidant. Its reactivity is influenced by multiple factors. The influence of fatty acids (FA; saturated, mono, and poly unsaturated acids from fish oil) binding to HSA, on copper(II) binding affinity and Cys34 thiol group accessibility/reactivity, in the presence of carbonylation agent (methylglyoxal, MG) was examined. HSA–copper(II) content, thiol group reactivity, and HSA carbonylation level were monitored spectrophotometrically. Changes in HSA were followed by fluorescence spectroscopy and native PAG electrophoresis. FA/HSA molar ratio was screened by GC. Together, binding of copper(II) ions and FA to HSA increase the reactivity of Cys34 thiol group (depending on the type of FA), with constant contribution of copper(II) ions of one-third. Carbonylation of FA–HSA–Cu(II) complexes caused a decrease in the Cys34 thiol group content, accompanied by a decrease in the content of HSA-bound copper. The carbonylation level of guanidine groups was not affected by FAs and copper(II) binding. Fluorescent emission spectra of FA–HSA–Cu(II)–MG complexes showed conformational changes in HSA molecule. Although binding of fatty acids and copper ions caused a significant increase in the thiol group reactivity, Cys34 thiol from FA–HSA–Cu(II) complexes reacted with MG in smaller extent than expected, probably as a consequence of conformational changes introduced by carbonylation. Increase in the percentage of reacted-free thiol groups with MG (due to FA and copper binding) may not seem to be very significant, but it is very important in complex biological systems, where catalytic metal is present.

Graphical abstract

Keywords

Human serum albumin Copper(II) Fatty acids Carbonylation HSA Cys34 thiol group reactivity 

Abbreviations

FA

Fatty acids

Myr

Myristic acid

Ole

Oleic acid

Ste

Stearic acid

FO

Fish oil fatty acids

HSA

Human serum albumin

Def HSA

Defatted HSA

FA–HSA–Cu(II)

Complex HSA with FA and copper(II) ion

HSA–SH

Cys34 free thiol group of HSA

MG

Methylglyoxal

HSA–MG

HSA modified with MG

DTNB

(5,5′dithiobis-(2-nitrobenzoic acid)

Notes

Acknowledgements

This work was supported by The Ministry of Education, Science and Technological Development of Serbia with Grant no. 172049. The authors acknowledge support of the FP7 RegPot Project FCUB ERA GA no. 256716.

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Copyright information

© SBIC 2018

Authors and Affiliations

  • Ana Z. Penezić
    • 2
    Email author
  • Jelena M. Aćimović
    • 1
  • Ivan D. Pavićević
    • 1
  • Vesna B. Jovanović
    • 1
  • Marija Takić
    • 3
  • Ljuba M. Mandić
    • 1
  1. 1.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  2. 2.Institute for the Application of Nuclear Energy, INEPBelgradeSerbia
  3. 3.Laboratory for Nutrition and Metabolism, Institute for Medical ResearchUniversity of BelgradeBelgradeSerbia

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