The role of citrate, lactate and transferrin in determining titanium release from surgical devices into human serum

  • Justin P. Curtin
  • Minji Wang
  • Tianfan Cheng
  • Lijian Jin
  • Hongzhe Sun
Original Paper

Abstract

The presence of ionic titanium in the serum of patients with titanium implants is currently unexplained. This is presumed due to corrosion, and yet the serum titanium concentration measured in patients is far greater than that predicted by its solubility. The binding of titanium ion as Ti(IV) to human transferrin (hTF) in serum indicates that Ti(IV) ions interact with human physiology. This is an intriguing finding since there is currently no known role for titanium ions in human physiology. Thus, understanding the factors that determine in vivo titanium ion release is relevant to further understanding this metal’s interactions with human biochemistry. The present study sought to determine the extent of titanium ion release of into human serum in vitro, and the role of citrate, lactate and hTF in this process. It was found that, when surgical devices of commercially pure titanium were placed into human serum, citrate and lactate concentrations were the prime determinants of titanium release. Crystallography revealed Ti(IV) bound to hTF in the presence of citrate alone, signalling that citrate can act as an independent ligand for Ti(IV) binding to hTF. Based on these findings, a two-stage process of titanium ion release into human serum that is dependent upon both citrate and hTF is proposed to explain the ongoing presence of titanium ion in human subjects with implanted titanium devices.

Keywords

Titanium Corrosion Transferrin Citrate Lactate 

Abbreviations

AO

Synthes Craniofacial Modular System—AO Foundation

CIT

Citrate

HEPES

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

HS

Human serum

hTF

Human serum transferrin

ICP-OES

Inductively coupled plasma-optical emission spectroscopy

Lac

Lactate

PEG

Poly(ethylene glycol)

SEM

Scanning electron microscopy

Tris

Tris(hydroxymethyl)aminomethane

Notes

Acknowledgements

We thank Prof. Quan Hao (School of Biomedical Sciences, HKU) for help in X-ray diffraction data collection and Shanghai Synchrotron Radiation Facility (Shanghai Institute of Applied Physics, Chinese Academy of Sciences, China PR) and their staff for providing beam-time and the support at the BL17U1 station (SSRF) for their kind help and H. Y. Tong (Dentistry, HKU), C. L. Yu (Dentistry, HKU), Paul K. D. Lee (Dentistry, HKU) and S. W. Lee (Dentistry, HKU) for their help with our experiments. The authors thank the Modern Dental Laboratory/the University of Hong Kong (HKU) Endowment Fund to L.J. Jin and the Research Grants Council of HKSAR and the University of Hong Kong (Seed funding for Basic Research (201504159007)) for support.

Supplementary material

775_2018_1557_MOESM1_ESM.docx (3.1 mb)
Supplementary material 1 (DOCX 3186 kb)

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Copyright information

© SBIC 2018

Authors and Affiliations

  • Justin P. Curtin
    • 1
  • Minji Wang
    • 1
  • Tianfan Cheng
    • 1
  • Lijian Jin
    • 1
  • Hongzhe Sun
    • 2
  1. 1.Faculty of DentistryThe University of Hong KongHong KongChina
  2. 2.Department of ChemistryThe University of Hong KongHong KongChina

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