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JBIC Journal of Biological Inorganic Chemistry

, Volume 20, Issue 6, pp 935–948 | Cite as

Characterization of antiproliferative potential and biological targets of a copper compound containing 4′-phenyl terpyridine

  • Ana Soraia Mendo
  • Sara Figueiredo
  • Catarina Roma-Rodrigues
  • Paula A. Videira
  • Zhen Ma
  • Mário Diniz
  • Miguel Larguinho
  • Pedro M. Costa
  • João C. Lima
  • Armando J. L. Pombeiro
  • Pedro V. Baptista
  • Alexandra R. Fernandes
Original Paper

Abstract

Several copper complexes have been assessed as anti-tumor agents against cancer cells. In this work, a copper compound [Cu(H2O){OS(CH3)2}L](NO3)2 incorporating the ligand 4′-phenyl-terpyridine antiproliferative activity against human colorectal, hepatocellular carcinomas and breast adenocarcinoma cell lines was determined, demonstrating high cytotoxicity. The compound is able to induce apoptosis and a slight delay in cancer cell cycle progression, probably by its interaction with DNA and induction of double-strand pDNA cleavage, which is enhanced by oxidative mechanisms. Moreover, proteomic studies indicate that the compound induces alterations in proteins involved in cytoskeleton maintenance, cell cycle progression and apoptosis, corroborating its antiproliferative potential.

Keywords

Anticancer drug Apoptosis Biomedicine DNA damage Nucleic acid 

Notes

Acknowledgments

We thank FCT/MEC for financial support (PTDC/BBB-NAN/1812/2012; PEst-OE/QUI/UI0100/2013). We also thank A. Silva, J. Palma, L. Coito and J. Silva for technical support; and G. Cabral (CEDOC, FCM/UNL) for technical support with flow cytometry assays.

Supplementary material

775_2015_1277_MOESM1_ESM.pdf (681 kb)
Supplementary material 1 (PDF 681 kb)

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Copyright information

© SBIC 2015

Authors and Affiliations

  • Ana Soraia Mendo
    • 1
  • Sara Figueiredo
    • 1
    • 2
  • Catarina Roma-Rodrigues
    • 1
  • Paula A. Videira
    • 1
    • 3
  • Zhen Ma
    • 4
  • Mário Diniz
    • 5
  • Miguel Larguinho
    • 2
    • 5
  • Pedro M. Costa
    • 6
  • João C. Lima
    • 7
  • Armando J. L. Pombeiro
    • 4
  • Pedro V. Baptista
    • 1
    • 2
  • Alexandra R. Fernandes
    • 1
    • 4
  1. 1.Departamento de Ciências da Vida, UCIBIOFaculdade de Ciências e Tecnologia da Universidade Nova de LisboaCaparicaPortugal
  2. 2.Departamento de Ciências da Vida, Nanomedicine@FCT, CIGMH, UCIBIO, Faculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparicaPortugal
  3. 3.CEDOC, Faculdade de Ciências MédicasUniversidade Nova de LisboaLisbonPortugal
  4. 4.Centro de Química Estrutural, Complexo 1, Instituto Superior TécnicoUniversidade de LisboaLisbonPortugal
  5. 5.UCIBIO, REQUIMTE, Faculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparicaPortugal
  6. 6.Departamento de Ciências e Engenharia do Ambiente, MARE-Marine and Environmental Sciences Centre, Faculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparicaPortugal
  7. 7.LAQV, REQUIMTE, Faculdade de Ciências e TecnologiaUniversidade Nova de LisboaCaparicaPortugal

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