JBIC Journal of Biological Inorganic Chemistry

, Volume 18, Issue 8, pp 895–903 | Cite as

The mechanism of copper uptake by tyrosinase from Bacillus megaterium

  • Margarita Kanteev
  • Mor Goldfeder
  • Michał Chojnacki
  • Noam Adir
  • Ayelet Fishman
Original Paper

Abstract

Tyrosinase belongs to the type 3 copper enzyme family, containing a dinuclear copper center, CuA and CuB. It is mainly responsible for melanin production in a wide range of organisms. Although copper ions are essential for the activity of tyrosinase, the mechanism of copper uptake is still unclear. We have recently determined the crystal structure of tyrosinase from Bacillus megaterium (TyrBm) and revealed that this enzyme has tighter binding of CuA in comparison with CuB. Investigating copper accumulation in TyrBm, we found that the presence of copper has a more significant effect on the diphenolase activity. By decreasing the concentration of copper, we increased the diphenolase to monophenolase activity ratio twofold. Using a rational design approach, we identified five variants having an impact on copper uptake. We have found that a major role of the highly conserved Asn205 residue is to stabilize the orientation of the His204 imidazole ring in the binding site, thereby promoting the correct coordination of CuB. Further investigation of these variants revealed that Phe197, Met61, and Met184, which are located at the entrance to the binding site, not only play a role in copper uptake, but are also important for enhancing the diphenolase activity. We propose a mechanism of copper accumulation by the enzyme as well as an approach to changing the selectivity of TyrBm towards l-dopa production.

Graphical abstract

Keywords

Tyrosinase Copper accumulation Bacillus megaterium Suicide inactivation 

Abbreviations

BCA

Bicinchoninic acid

DSC

Differential scanning calorimetry

ICP-AES

Inductively coupled plasma atomic emission spectroscopy

PDB

Protein Data Bank

Tris

Tris(hydroxymethyl)aminomethane

TyrBm

Tyrosinase from Bacillus megaterium

TyrSc

Tyrosinase from Streptomyces castaneoglobisporus

Supplementary material

775_2013_1034_MOESM1_ESM.pdf (476 kb)
Supplementary material 1 (PDF 475 kb)

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Copyright information

© SBIC 2013

Authors and Affiliations

  • Margarita Kanteev
    • 1
  • Mor Goldfeder
    • 1
  • Michał Chojnacki
    • 1
  • Noam Adir
    • 2
  • Ayelet Fishman
    • 1
  1. 1.Department of Biotechnology and Food EngineeringTechnion-Israel Institute of TechnologyHaifaIsrael
  2. 2.Schulich Faculty of ChemistryTechnion-Israel Institute of TechnologyHaifaIsrael

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