JBIC Journal of Biological Inorganic Chemistry

, Volume 17, Issue 6, pp 961–974 | Cite as

Identification of [CuCl(acac)(tmed)], a copper(II) complex with mixed ligands, as a modulator of Cu,Zn superoxide dismutase (Sod1p) activity in yeast

  • Ioana Dumitru
  • Cristian D. Ene
  • Augustin M. Ofiteru
  • Codruta Paraschivescu
  • Augustin M. Madalan
  • Ion Baciu
  • Ileana C. Farcasanu
Original Paper

Abstract

Superoxide dismutases (SODs) stand in the prime line of enzymatic antioxidant defense in nearly all eukaryotic cells exposed to oxygen, catalyzing the breakdown of the superoxide anionic radical to O2 and H2O2. Overproduction of superoxide correlates with numerous pathophysiological conditions, and although the native enzyme can be used as a therapeutic agent in superoxide-associated conditions, synthetic low molecular weight mimetics are preferred in terms of cost, administration mode, and bioavailability. In this study we make use of the model eukaryote Saccharomyces cerevisiae to investigate the SOD-mimetic action of a mononuclear mixed-ligand copper(II) complex, [CuCl(acac)(tmed)] (where acac is acetylacetonate anion and tmed is N,N,N′,N′-tetramethylethylenediamine). Taking advantage of an easily reproducible phenotype of yeast cells which lack Cu–Zn SOD (Sod1p), we found that the compound could act either as a superoxide scavenger in the absence of native Sod1p or as a Sod1p modulator which behaved differently under various genetic backgrounds.

Keywords

Superoxide dismutase SOD1 modulator Cu(II) complex Saccharomyces cerevisiae Oxidative stress 

Abbreviations

acac

Acetylacetonate anion

Cu,Zn-SOD

Copper- and zinc-containing superoxide dismutase

MES

2-(N-Morpholino)ethanesulfonic acid

Mn-SOD

Manganese-containing superoxide dismutase

MOPS

3-(N-Morpholino)propanesulfonic acid

NBT

Nitro blue tetrazolium

OD600

Optical density of the cellular suspension at 600 nm

PQ

Paraquat

ROS

Reactive oxygen species

SOD

Superoxide dismutase

SD

Synthetic complete medium containing 2 % dextrose

SD-K

Synthetic complete medium containing 2 % dextrose lacking lysine

tmed

N,N,N′,N′-Tetramethylethylenediamine

Tris

Tris(hydroxymethyl)methylamine

YPD

Yeast extract–peptone–dextrose

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Copyright information

© SBIC 2012

Authors and Affiliations

  • Ioana Dumitru
    • 1
  • Cristian D. Ene
    • 1
  • Augustin M. Ofiteru
    • 1
  • Codruta Paraschivescu
    • 1
  • Augustin M. Madalan
    • 2
  • Ion Baciu
    • 1
  • Ileana C. Farcasanu
    • 1
  1. 1.Department of Organic Chemistry, Biochemistry and Catalysis, Faculty of ChemistryUniversity of BucharestBucharestRomania
  2. 2.Department of Inorganic ChemistryUniversity of BucharestBucharestRomania

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