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JBIC Journal of Biological Inorganic Chemistry

, Volume 12, Issue 2, pp 194–203 | Cite as

Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I

  • Alexandra R. Stettler
  • Valérie Chaurin
  • Edwin C. Constable
  • Catherine E. Housecroft
  • Maria A. SchwarzEmail author
Original Paper

Abstract

The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.

Keywords

Affinity capillary electrophoresis Buffer Oligonucleotide Metal cation 

Notes

Acknowledgements

We would like to thank Shimadzu Corporation and the University of Basel for their kind support. We also thank Yorck-Michael Neuhold for constructive discussions. Partial funding for this project was provided by Swiss National Science Foundation, grant numbers 200021-103812/1 and 20C321-101122.

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Copyright information

© SBIC 2006

Authors and Affiliations

  • Alexandra R. Stettler
    • 1
  • Valérie Chaurin
    • 1
  • Edwin C. Constable
    • 1
  • Catherine E. Housecroft
    • 1
  • Maria A. Schwarz
    • 1
    Email author
  1. 1.Department of ChemistryUniversity of BaselBaselSwitzerland

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