Investigations of differences in iron oxidation state inside single neurons from substantia nigra of Parkinson’s disease and control patients using the micro-XANES technique
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X-ray absorption near edge structure spectroscopy was applied in order to investigate differences in iron chemical state between the nerve cells of substantia nigra (SN) representing Parkinson’s disease (PD) and those of control cases. Autopsy samples were cut using a cryotome, and were not fixed and not embedded in paraffin. The comparison of the absorption spectra near the iron K-edge measured in melanized neurons from SN of PD and control samples did not show significant differences in iron oxidation state. Measurements of inorganic reference materials containing iron in the second and third oxidation states indicate that most of the iron in all the nerve cell bodies examined was oxidized and occurred as trivalent ferric iron (Fe3+).
KeywordsParkinson’s disease Iron oxidation state X-ray absorption near edge structure spectroscopy Single cell
This work was supported by the Ministry of Education and Science (Warsaw, Poland) and the following grants: Ministry of Education and Science grants 112/E-356/SPB/DESY/P-05/DWM728/2003-2005 and 3T11E01029; IHP-Contract HPRI-CT-1999-00040/2001-00140 of the European Commission; the European Community Research Infrastructure Action under FP6 “Structuring the European Research Area” (through the Integrated Infrastructure Initiative “Integrating Activity on Synchrotron and Free Electron Laser Science”); and HASYLAB experimental grant II-02-092.
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