JBIC Journal of Biological Inorganic Chemistry

, Volume 11, Issue 6, pp 725–734 | Cite as

Fast cadmium inhibition of photosynthesis in cyanobacteria in vivo and in vitro studies using perturbed angular correlation of γ-rays

  • Klára Nárcisz Sas
  • László Kovács
  • Ottó Zsίros
  • Zoltán Gombos
  • Győző Garab
  • Lars Hemmingsen
  • Eva Danielsen
Original Paper


The effect of cadmium on the photosynthetic activity of Synechocystis PCC 6803 was monitored in this study. The oxygen evolving capacity of Synechocystis treated with 40 μM CdCl2 was depressed to 10% of the maximum in 15 min, indicating that Cd2+ penetrated rapidly into the cells and blocked the photosynthetic activity. However, neither photosystem II (PSII) nor photosystem I (PSI) activity showed a significant short-term decrease which would explain this fast decrease in the whole-chain electron transport. Thermoluminescence measurements have shown that the charge separation and stabilization in PSII remains essentially unchanged during the first few hours following the Cd2+ treatment. The electron flow through PSI was monitored by following the redox changes of the P700 reaction centers of PSI. Alterations in the oxidation kinetics of P700 in the Cd2+-treated cells indicated that Cd2+ treatment might affect the available electron acceptor pool of P700, including the CO2 reduction and accumulation in the cells. Perturbed angular correlation of γ-rays (PAC) using the radioactive 111mCd isotope was used to follow the Cd2+ uptake at a molecular level. The most plausible interpretation of the PAC data is that Cd2+ is taken up by one or more Zn proteins replacing Zn2+ in Synechocystis PCC 6803. Using the radioactive 109Cd isotope, a protein of approximately 30 kDa that binds Cd2+ could be observed in sodium dodecyl sulfate polyacrylamide gel electrophoresis. The results indicate that Cd2+ might inactivate different metal-containing enzymes, including carbonic anhydrase, by replacing the zinc ion, which would explain the rapid and almost full inhibition of the photosynthetic activity in cyanobacteria.


Cyanobacteria Synechocystis sp. 6803 Cadmium toxicity Photosynthesis Oxygen-evolving activity 



Bauers axially symmetric independent ligands


Carbonic anhydrase


Carbon-concentrating mechanism


Electric field gradient


N-(2-Hydroxyethyl)piperazine)-N′-ethanesulfonic acid


Nuclear quadrupole interaction


Perturbed angular correlation of γ-rays


Polyacrylamide gel electrophoresis




Photosystem I


Photosystem II


Reduced secondary quinine acceptor


Ribulose bisphosphate carboxylase-oxygenase


Sodium dodecyl sulfate




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Copyright information

© SBIC 2006

Authors and Affiliations

  • Klára Nárcisz Sas
    • 1
  • László Kovács
    • 2
  • Ottó Zsίros
    • 2
  • Zoltán Gombos
    • 2
  • Győző Garab
    • 2
  • Lars Hemmingsen
    • 1
  • Eva Danielsen
    • 3
  1. 1.Department of Natural SciencesRoyal Veterinary and Agricultural UniversityFrederiksberg CDenmark
  2. 2.Institute of Plant Biology, Biological Research CenterHungarian Academy of SciencesSzegedHungary
  3. 3.Nano Science CenterUniversity of CopenhagenCopenhagen ØDenmark

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