JBIC Journal of Biological Inorganic Chemistry

, Volume 8, Issue 7, pp 726–732

The cellular distribution and oxidation state of platinum(II) and platinum(IV) antitumour complexes in cancer cells

  • Matthew D. Hall
  • Carolyn T. Dillon
  • Mei Zhang
  • Philip Beale
  • Zhonghou Cai
  • Barry Lai
  • Anton P. J. Stampfl
  • Trevor W. Hambley
Original Article

Abstract

The cellular distribution of platinum in A2780 ovarian cancer cells treated with cisplatin and platinum(IV) complexes with a range of reduction potentials has been examined using elemental analysis (synchrotron radiation-induced X-ray emission). The cellular distribution of platinum(IV) drugs after 24 h is similar to that of cisplatin, consistent with the majority of administered platinum(IV) drugs being reduced. Micro-X-ray absorption near-edge spectra of cells treated with cisplatin and platinum(IV) complexes confirmed the reduction of platinum(IV) to platinum(II). In cells treated, the most difficult to reduce complex, cis,trans,cis-[PtCl2(OH)2(NH3)2], platinum(IV) was detected in the cells along with platinum(II). The observations are in accordance with the relative ease of reduction of the platinum(IV) complexes used and support the requirement of reduction for activation of platinum(IV) complexes.

Keywords

Cellular distribution Cisplatin Ovarian cancer cells Platinum complexes Synchrotron radiation-induced X-ray emission 

Abbreviations

en

ethane-1,2-diamine

GM

growth medium

PBS

phosphate buffered saline

RPMI

Roswell Park Memorial Institute

SRIXE

synchrotron radiation-induced X-ray emission

XAFS

X-ray absorption fine structure

XANES

X-ray absorption near-edge spectroscopy

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Copyright information

© SBIC 2003

Authors and Affiliations

  • Matthew D. Hall
    • 1
  • Carolyn T. Dillon
    • 1
  • Mei Zhang
    • 2
  • Philip Beale
    • 3
  • Zhonghou Cai
    • 4
  • Barry Lai
    • 4
  • Anton P. J. Stampfl
    • 4
    • 5
  • Trevor W. Hambley
    • 1
  1. 1.Centre for Heavy Metals Research, School of Chemistry F11The University of SydneyAustralia
  2. 2.Department of Radiation OncologyRoyal Prince Alfred HospitalCamperdownAustralia
  3. 3.Department of Medical Oncology, Sydney Cancer CentreRoyal Prince Alfred HospitalCamperdownAustralia
  4. 4.Experimental Facilities DivisionArgonne National LaboratoryArgonneUSA
  5. 5.Physics DivisionAustralian Nuclear Science and Technology OrganisationLucas HeightsAustralia

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