Journal of Bone and Mineral Metabolism

, Volume 19, Issue 1, pp 56–60 | Cite as

Stromal cell activity in bone marrow from the tibia and iliac crest of patients with rheumatoid arthritis

  • Yoshihiro Suzuki
  • Kang Jung Kim
  • Shigeru Kotake
  • Tatsuo Itoh

Abstract

Bone marrow aspirates were obtained from the iliac crest and tibial epiphysis in 23 patients with rheumatoid arthritis (RA) who were undergoing total knee arthroplasty. The number of fibroblast colony-forming units (CFU-F), which contain osteogenic precursor cells, and alkaline phosphatase (ALP) activity, as a marker of the osteoblastic phenotype, were compared between the iliac and tibial marrow for each patient. The prevalence of CFU-F in tibial marrow was similar to that in iliac marrow (96% vs 100%, respectively). However, the average number of CFU-F per 4 × 105 bone marrow mononuclear cells was significantly lower in tibial marrow than in iliac marrow (8.2 vs 28.1, respectively; P < 0.01). Although ALP activity was detected in all iliac and tibial marrow specimens, it was significantly lower in tibial marrow compared with iliac marrow (3.7 vs 11.9 nmol/min/mg protein, respectively; P < 0.01). In addition, there was a significant correlation between the patient's age and the number of CFU-F in iliac marrow (r = −0.547; P < 0.01), although there was no correlation in tibial marrow. These results demonstrate that the osteogenic activity of bone marrow varies at different sites in patients with RA. The data may also contribute to further investigation into the differential effects of various disease processes on systemic as well as local stromal cell activity in bone marrow.

Key words rheumatoid arthritis bone marrow stromal cell activity iliac crest tibial epiphysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Tokyo 2001

Authors and Affiliations

  • Yoshihiro Suzuki
    • 1
  • Kang Jung Kim
    • 1
  • Shigeru Kotake
    • 2
  • Tatsuo Itoh
    • 1
  1. 1.Department of Orthopaedic Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, JapanJP
  2. 2.Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, JapanJP

Personalised recommendations