Effect of RAB31 silencing on osteosarcoma cell proliferation and migration through the Hedgehog signaling pathway

  • Qiong Yu
  • Dong Li
  • Dan Wang
  • Chun-Mei Hu
  • Yan Sun
  • Yan Tang
  • Guang ShiEmail author
Original Article


Osteosarcoma (OS) is a prevalent cancer that plagues people worldwide. Identifying prognostic markers would be useful in treating human OS. In this study, we aimed to explore the functions of Ras-related protein Rab-31 (RAB31) in OS-cell proliferation, migration, and invasion as well as its roles in the Hedgehog signaling pathway for better understanding of the mechanism. To assess the detailed regulatory mechanism of RAB31 silencing on OS, both RT-qPCR and Western blot analysis were employed to evaluate the expressions of RAB31 as well as the Hedgehog signaling pathway-related genes. Besides, we also investigated the effects of silenced RAB31 both in vitro and in vivo. First, we found that in OS tissues, both mRNA and protein expressions of RAB31 and PCNA had a significant increase. Second, the Hedgehog signaling pathway was detected to play an integral role in OS progression. Finally, after transfection of RAB31-siRNA to reduce the expression of RAB31, the Hedgehog signaling pathway was suppressed, along with cell proliferation, invasion, and migration. Therefore, we conclude that RAB31 plays an important role in OS development and its silencing delays the OS progression via suppression of the Hedgehog signaling pathway.


RAB31 Osteosarcoma Hedgehog signaling pathway Proliferation Invasion 



We thank the reviewers for critical comments.

Compliance with ethical standards

Conflicts of interest

The authors declares that they have conflict of interest.

Ethical approval

All aspects of the study are in strict accordance with the Declaration of Helsinki. All of the above specimens were collected with informed consent of patients and all the patients signed informed consent.


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Copyright information

© The Japanese Society for Bone and Mineral Research and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Qiong Yu
    • 1
  • Dong Li
    • 2
  • Dan Wang
    • 3
  • Chun-Mei Hu
    • 1
  • Yan Sun
    • 1
  • Yan Tang
    • 1
  • Guang Shi
    • 1
    Email author
  1. 1.Department of Hematology and OncologyThe Second Hospital of Jilin UniversityChangchunPeople’s Republic of China
  2. 2.Department of Obstetrics and GynecologyThe Second Hospital of Jilin UniversityChangchunPeople’s Republic of China
  3. 3.Department of Breast SurgeryThe Second Hospital of Jilin UniversityChangchunPeople’s Republic of China

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