Journal of Bone and Mineral Metabolism

, Volume 35, Issue 5, pp 497–503 | Cite as

Lithium chloride enhances bone regeneration and implant osseointegration in osteoporotic conditions

  • Yifan Jin
  • Lihua Xu
  • Xiaohui Hu
  • Shixian Liao
  • Janak L. Pathak
  • Jinsong Liu
Original Article


Osteoporotic patients have a high risk of dental and orthopedic implant failure. Lithium chloride (LiCl) has been reported to enhance bone formation. However, the role of LiCl in the success rate of dental and orthopedic implants in osteoporotic conditions is still unknown. We investigated whether LiCl enhances implant osseointegration, implant fixation, and bone formation in osteoporotic conditions. Sprague–Dawley female rats (n = 18) were ovariectomized (OVX) to induce osteoporosis, and another nine rats underwent sham surgery. Three months after surgery, titanium implants were implanted in the tibia of the OVX and sham group rats. After implantation, the OVX rats were gavaged with 150 mg/kg/2 days of LiCl (OVX + LiCl group) or saline (OVX group), and sham group rats were gavaged with saline for 3 months. Implant osseointegration and bone formation were analyzed using histology, biomechanical testing, and micro computed tomography (micro-CT). More bone loss was observed in the OVX group compared to the control, and LiCl treatment enhanced bone formation and implant fixation in osteoporotic rats. In the OVX group, bone−implant contact (BIC) was decreased by 81.2 % compared to the sham group. Interestingly, the OVX + LiCl group showed 4.4-fold higher BIC compared to the OVX group. Micro-CT data of tibia from the OVX + LiCl group showed higher bone volume, trabecular thickness, trabecular number, and osseointegration compared to the OVX group. Maximum push-out force and implant−bone interface shear strength were 2.9-fold stronger in the OVX + LiCl group compared to the OVX group. In conclusion, LiCl enhanced implant osseointegration, implant fixation, and bone formation in osteoporotic conditions, suggesting LiCl as a promising therapeutic agent to prevent implant failure and bone loss in osteoporotic conditions.


Osteoporosis Lithium chloride Osseointegration Implant fixation Bone formation 



The authors would like to acknowledge the support provided by the Nature Science Foundation of Zhejiang Province (No. LY1 3H140005), and Grants from the Health and Family Planning Commission of Zhejiang Province, China (No. 2013KYA124), the Scientific Research Fund of Zhejiang Provincial Education Department (No. Y201326860) and the Wenzhou Science and Technology Bureau (Nos. Y20110108 and 20130033).

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.


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Copyright information

© The Japanese Society for Bone and Mineral Research and Springer Japan 2016

Authors and Affiliations

  1. 1.School and Hospital of StomatologyWenzhou Medical UniversityWenzhouChina
  2. 2.The First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina
  3. 3.Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology (SPST)Tianjin UniversityTianjinChina

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