Crystallographic orientation of the c-axis of biological apatite as a new index of the quality of subchondral bone in knee joint osteoarthritis
The aim of the present study was to investigate the preferred orientation of biological apatite (BAp) as a new index of the quality of subchondral bone (SB) in knee joint osteoarthritis (OA). Ten OA and five normal knee joints were obtained. Thickness, quantity and bone mineral density (BMD) of SB were analyzed at the medial condyle of the femur in dry conditions by peripheral quantitative computed tomography. In addition, the preferred crystallographic orientation of the c-axis of BAp was evaluated as bone quality parameter using a microbeam X-ray diffractometer technique. BMD and thickness of SB were significantly increased in OA specimens compared to normal knee specimens (P < 0.01), and the preferred orientation of the c-axis of BAp along the normal direction of SB surface was significantly higher in OA specimens (P < 0.01), reflecting the change in stress of concentration in the pathological portion without cartilage. SB sclerosis in OA results in both proliferation of bone tissues and enhanced degree of preferential alignment of the c-axis of BAp. Our findings could have major implications for the diagnosis of clinical studies, including pathologic elucidation in OA.
KeywordsOsteoarthritis (OA) Apatite orientation Bone quality Microbeam X-ray diffractometer (μXRD)
The authors would like to thank Prof. Yuji Nakajima and Prof. Hiroshi Kiyama, Osaka City University for providing the bone specimens. We wish to thank the family of the donor for the generosity in the face of the bereavement. This work was supported by Grants-in-Aid for Scientific Research (S) from the Japan Society for Promotion of Science (Grant No. 25220912) and basic science research program through the National Research Foundation of Korea (NRF) (2009-0093814).
Compliance with ethical standards
Conflict of interest
None of the authors has competing interests to declare.
- 4.Elliott JC, Wilson RM, Dowker SEP (2002) Apatite structures. Adv X-Ray Anal 45:172–181Google Scholar
- 14.Huebner JL, Bay-Jensen AC, Huffman KM, He Y, Leeming DJ, McDaniel GE, Karsdal MA, Kraus VB (2014) Alpha C-telopeptide of type I collagen is associated with subchondral bone turnover and predicts progression of joint space narrowing and osteophytes in osteoarthritis. Arthritis Rheum 66:2440–2449CrossRefGoogle Scholar
- 15.Yusup A, Kaneko H, Liu L, Ning L, Sadatsuki R, Hada S, Kamagata K, Kinoshita M, Futami I, Shimura Y, Tsuchiya M, Saita Y, Takazawa Y, Ikeda H, Aoki S, Kaneko K, Ishijima M (2015) Bone marrow lesions, subchondral bone cysts and subchondral bone attrition are associated with histological synovitis in patients with end-stage knee osteoarthritis: a cross-sectional study. Osteoarthritis Cartilage. doi: 10.1016/j.joca.2015.05.017 PubMedGoogle Scholar
- 17.Kraus VB, Feng S, Wang S, White S, Ainslie M, Graverand MP, Brett A, Eckstein F, Hunter DJ, Lane NE, Taljanovic MS, Schnitzer T, Charles HC (2013) Subchondral bone trabecular integrity predicts and changes concurrently with radiographic and magnetic resonance imaging-determined knee osteoarthritis progression. Arthritis Rheum 65:1812–1821CrossRefPubMedPubMedCentralGoogle Scholar
- 39.Neogi T, Felson D, Niu J, Lynch J, Nevitt M, Guermazi A, Roemer F, Lewis CE, Wallace B, Zhang Y (2009) Cartilage loss occurs in the same subregions as subchondral bone attrition: a within-knee subregion-matched approach from the Multicenter Osteoarthritis Study. Ann Rheum Dis 61:1539–1544Google Scholar
- 40.Radin EL, Rose RM (1986) Role of subchondral bone in the initiation and progression of cartilage damage. Clin Orthop Relat Res 213:34–40Google Scholar
- 41.Holzer N, Salvo D, Marijnissen ACA, Vincken KL, Ahmad AC, Serra E, Hoffmeyer P, Stern R, Lübbeke A, Assal M (2015) Radiographic evaluation of posttraumatic osteoarthritis of the ankle: the Kellgren–Lawrence scale is reliable and correlates with clinical symptoms. Osteoarthritis Cartilage 23:363–369CrossRefPubMedGoogle Scholar
- 42.Wong AKO, Beattie KA, Emond PD, Inglis D, Duryea J, Doan A, Ioannidis G, Webber CE, O’Neill J, de Beer J, Adachi JD, Papaioannou A (2009) Quantitative analysis of subchondral sclerosis of the tibia by bone texture parameters in knee radiographs: site-specific relationships with joint space width. Osteoarthritis Cartilage 17:1453–1460CrossRefPubMedPubMedCentralGoogle Scholar
- 43.Shiraishi A, Miyabe S, Nakano T, Umakoshi Y, Ito M, Mihara M (2009) The combination therapy with alfacalcidol and risedronate improves the mechanical property in lumbar spine by affecting the material properties in an ovariectomized rat model of osteoporosis. BMC Musculoskelet Disord 10:66CrossRefPubMedPubMedCentralGoogle Scholar