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Journal of Bone and Mineral Metabolism

, Volume 34, Issue 4, pp 422–428 | Cite as

Chlamydia pneumoniae and Helicobacter pylori IgG seropositivities are not predictors of osteoporosis-associated bone loss: a prospective cohort study

  • Mohammad Reza Kalantarhormozi
  • Majid Assadi
  • Katayoun Vahdat
  • Kamyar Asadipooya
  • Afshin Ostovar
  • Katayoun Raissi
  • Hossein Darabi
  • Shokrollah Farrokhi
  • Sina Dobaradaran
  • Maryam Farrokhnia
  • Iraj NabipourEmail author
Original Article

Abstract

The potential link between infection with Chlamydia pneumoniae or Helicobacter pylori and osteoporosis has not been investigated in population-based longitudinal studies. A total of 250 healthy postmenopausal women who participated in a prospective cohort study were evaluated for IgG antibodies directed against C. pneumoniae and H. p ylori, osteoprotegerin (OPG), the receptor activator of nuclear factor kappa B ligand (RANKL), CrossLaps, and osteocalcin. Bone mineral density (BMD) was measured at the femoral neck and lumbar spine at baseline and at follow-up 5.8 years later. There were no significant differences in age-adjusted bone turnover markers, OPG, RANKL, the RANKL/OPG ratio, and BMD between the C. p neumoniae and H. p ylori IgG seropositive and seronegative subjects (P > 0.05). Neither C. p neumoniae nor H. p ylori IgG seropositivity was associated with age-and body mass index-adjusted BMD at the femoral neck and lumbar spine or bone loss at the 5.8-year follow-up. In logistic regression analysis, neither C. p neumoniae nor H. p ylori IgG seropositivities predicted incident lumbar or spine osteoporosis 5.8 years later. In conclusion, neither C. p neumoniae nor H. p ylori IgG seropositivity was associated with bone turnover markers, the RANKL/OPG ratio, BMD, or bone loss in postmenopausal women. In addition, chronic infection with C. p neumoniae or H. p ylori did not predict incident osteoporosis among this group of women.

Keywords

Chlamydia pneumoniae Helicobacter pylori Bone mineral density Osteoporosis 

Notes

Acknowledgments

This project was supported jointly by a grant from Bushehr University of Medical Sciences and Health Services; the Ministry of Health; Tehran Endocrine Research Center; Tehran University of Medical Science, Tehran, Islamic Republic of Iran; and Bushehr Province Research Committee (PGHRC: 1385.234.345). The authors thank Dr. Syed Reza Imami, Shiva Mosadeghzadeh, and Zahra Amiri for assistance in the field work.

Conflict of interest

An award was given to Iraj Nabipour by “Novo Nordisk Pars” (The Best Innovator of Diabetes in Iran 2013). All other authors have no conflicts of interest.

References

  1. 1.
    Watson C, Alp NJ (2008) Role of Chlamydia pneumoniae in atherosclerosis. Clin Sci (Lond) 114:509–531CrossRefGoogle Scholar
  2. 2.
    Campbell LA, Kuo CC (2002) Chlamydia pneumoniae pathogenesis. J Med Microbiol 51:623–625CrossRefPubMedGoogle Scholar
  3. 3.
    Bailey L, Engstrom P, Nordstrom A, Bergstrom S, Waldenstrom A, Nordstrom P (2008) Chlamydia pneumoniae infection results in generalized bone loss in mice. Microbes Infect 10:1175–1181CrossRefPubMedGoogle Scholar
  4. 4.
    Rizzo A, Di Domenico M, Carratelli CR, Mazzola N, Paolillo R (2011) Induction of proinflammatory cytokines in human osteoblastic cells by Chlamydia pneumoniae. Cytokine 56:450–457CrossRefPubMedGoogle Scholar
  5. 5.
    Di Pietro M, Schiavoni G, Sessa V, Pallotta F, Costanzo G, Sessa R (2013) Chlamydia pneumoniae and osteoporosis-associated bone loss: a new risk factor? Osteoporos Int 24:1677–1682CrossRefPubMedGoogle Scholar
  6. 6.
    Gasbarrini A, Franceschi F, Armuzzi A, Ojetti V, Candelli M, Torre ES, De Lorenzo A, Anti M, Pretolani S, Gasbarrini G (1999) Extradigestive manifestations of Helicobacter pylori gastric infection. Gut 45(Suppl 1):I9–I12CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Papamichael KX, Papaioannou G, Karga H, Roussos A, Mantzaris GJ (2009) Helicobacter pylori infection and endocrine disorders: is there a link? World J Gastroenterol 15:2701–2707CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Ozdem S, Akcam M, Yilmaz A, Gultekin M, Artan R (2007) Biochemical markers of bone metabolism in children with Helicobacter pylori infection. Dig Dis Sci 52:967–972CrossRefPubMedGoogle Scholar
  9. 9.
    Figura N, Gennari L, Merlotti D, Lenzi C, Campagna S, Franci B, Lucani B, Trabalzini L, Bianciardi L, Gonnelli C, Santucci A, Nut A (2005) Prevalence of Helicobacter pylori infection in male patients with osteoporosis and controls. Dig Dis Sci 50:847–852CrossRefPubMedGoogle Scholar
  10. 10.
    Kakehasi AM, Rodrigues CB, Carvalho AV, Barbosa AJ (2009) Chronic gastritis and bone mineral density in women. Dig Dis Sci 54:819–824CrossRefPubMedGoogle Scholar
  11. 11.
    Kakehasi AM, Mendes CM, Coelho LG, Castro LP, Barbosa AJ (2007) The presence of Helicobacter pylori in postmenopausal women is not a factor to the decrease of bone mineral density. Arq Gastroenterol 44:266–270CrossRefPubMedGoogle Scholar
  12. 12.
    Tohidi M, Akbarzadeh S, Larijani B, Kalantarhormozi M, Ostovar A, Assadi M, Vahdat K, Farrokhnia M, Sanjdideh Z, Amirinejad R, Nabipour I (2012) Omentin-1, visfatin and adiponectin levels in relation to bone mineral density in Iranian postmenopausal women. Bone 51:876–881CrossRefPubMedGoogle Scholar
  13. 13.
    World Health Organization (1994) Assessment of fracture risk and its application to screening for postmenopausal osteoporosis. Report of a WHO Study Group. World Health Organ Tech Rep Ser 843:1–129Google Scholar
  14. 14.
    Maass M, Gieffers J, Krause E, Engel PM, Bartels C, Solbach W (1998) Poor correlation between microimmunofluorescence serology and polymerase chain reaction for detection of vascular Chlamydia pneumoniae infection in coronary artery disease patients. Med Microbiol Immunol 187:103–106CrossRefPubMedGoogle Scholar
  15. 15.
    Danesh J, Whincup P, Lewington S, Walker M, Lennon L, Thomson A, Wong YK, Zhou X, Ward M (2002) Chlamydia pneumoniae IgA titres and coronary heart disease; prospective study and meta-analysis. Eur Heart J 23:371–375CrossRefPubMedGoogle Scholar
  16. 16.
    Al-Sammak F, Kalinski T, Weinert S, Link A, Wex T, Malfertheiner P (2013) Gastric epithelial expression of IL-12 cytokine family in Helicobacter pylori infection in human: is it head or tail of the coin? PLoS One 8:e75192CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Romero-Adrian TB, Leal-Montiel J, Monsalve-Castillo F, Mengual-Moreno E, McGregor EG, Perini L, Antunez A (2010) Helicobacter pylori: bacterial factors and the role of cytokines in the immune response. Curr Microbiol 60:143–155CrossRefPubMedGoogle Scholar
  18. 18.
    Hofbauer LC, Kuhne CA, Viereck V (2004) The OPG/RANKL/RANK system in metabolic bone diseases. J Musculoskelet Neuronal Interact 4:268–275PubMedGoogle Scholar
  19. 19.
    Nabipour I, Larijani B, Vahdat K, Assadi M, Jafari SM, Ahmadi E, Movahed A, Moradhaseli F, Sanjdideh Z, Obeidi N, Amiri Z (2009) Relationships among serum receptor of nuclear factor-kappaB ligand, osteoprotegerin, high-sensitivity C-reactive protein, and bone mineral density in postmenopausal women: osteoimmunity versus osteoinflammatory. Menopause 16:950–955CrossRefPubMedGoogle Scholar
  20. 20.
    Davidson M, Kuo CC, Middaugh JP, Campbell LA, Wang SP, Newman WP 3rd, Finley JC, Grayston JT (1998) Confirmed previous infection with Chlamydia pneumoniae (TWAR) and its presence in early coronary atherosclerosis. Circulation 98:628–633CrossRefPubMedGoogle Scholar

Copyright information

© The Japanese Society for Bone and Mineral Research and Springer Japan 2015

Authors and Affiliations

  • Mohammad Reza Kalantarhormozi
    • 1
  • Majid Assadi
    • 2
  • Katayoun Vahdat
    • 3
  • Kamyar Asadipooya
    • 4
  • Afshin Ostovar
    • 3
  • Katayoun Raissi
    • 1
  • Hossein Darabi
    • 3
  • Shokrollah Farrokhi
    • 3
  • Sina Dobaradaran
    • 3
  • Maryam Farrokhnia
    • 5
  • Iraj Nabipour
    • 5
    • 6
    Email author
  1. 1.Department of Endocrine and Metabolic Diseases, The Persian Gulf Tropical Medicine Research CentreBushehr University of Medical SciencesBushehrIran
  2. 2.The Persian Gulf Nuclear Medicine Research CentreBushehr University of Medical SciencesBushehrIran
  3. 3.Department of Infectious Diseases, The Persian Gulf Tropical Medicine Research CentreBushehr University of Medical SciencesBushehrIran
  4. 4.Division of Endocrinology and MetabolismUniversity of Kansas Medical CenterKansas CityUSA
  5. 5.Department of Biochemistry, The Persian Gulf Marine Biotechnology Research CentreBushehr University of Medical SciencesBushehrIran
  6. 6.The Persian Gulf Tropical Medicine Research CenterBushehrIran

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