Fructus Ligustri Lucidi (FLL) ethanol extract increases bone mineral density and improves bone properties in growing female rats
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Osteoporosis is a chronic disease affecting millions of people worldwide. It is generally accepted that acquisition of a high peak bone mass (PBM) early in life can reduce the risk of osteoporosis later in life. The aims of this study were to investigate the effects of Fructus Ligustri Lucidi (FLL) ethanol extract on bone mineral density and its mechanical properties in growing female rats and to explore the underlying mechanisms. The rats were given different doses of FLL extract mixed with AIN-93G formula (0.40, 0.65 and 0.90 %), and a group given AIN-93G diet treatment only was used as control. The intervention lasted for 16 weeks until the animals were about 5 months old, the time when the animals almost reach their PBM. Our results showed that FLL treatment increased bone mineral density and improved bone mechanical properties in the growing female rats in a dose-dependent manner. In addition, FLL treatment significantly decreased the serum bone-resorbing marker, CTX-I, while significantly increasing serum 25(OH)D3 and thereby increasing Ca absorption and Ca retention. Intriguingly, both in vivo and in vitro results demonstrated that FLL treatment could reduce the RANKL/OPG ratio. In conclusion, FLL ethanol extract exerted beneficial effects on peak bone mass acquisition and the improvement of bone mechanical properties by favoring Ca metabolism and decreasing the RANKL/OPG ratio.
KeywordsFructus Ligustri Lucidi Calcium balance Bone mineral density Bone properties RANKL/OPG
Conflict of interest
All authors have no conflicts of interest.
- 1.WHO (2003) Prevention and management of osteoporosis. World Health Organ Tech Rep Ser 921:1–164Google Scholar
- 2.NIH (2001) NIH consensus development panel on osteoporosis prevention, diagnosis, and therapy, March 7–29, 2000: highlights of the conference. South Med J 94:569–573Google Scholar
- 4.Xue K, Ren-hui L, Xiu-juan W (2012) Advances on studies of anti-osteoporosis applications and mechanisms by Herba Epimedii and Fructus ligustri lucidi. Chin J Exp Tradit Med Formulae 18:331–334Google Scholar
- 11.Sengupta S, Arshad M, Sharma S, Dubey M, Singh MM (2005) Attainment of peak bone mass and bone turnover rate in relation to estrous cycle, pregnancy and lactation in colony-bred Sprague–Dawley rats: suitability for studies on pathophysiology of bone and therapeutic measures for its management. J Steroid Biochem Mol Biol 94:421–429PubMedCrossRefGoogle Scholar
- 22.Srivastava K, Khan K, Tyagi AM, Khan MP, Yadav DK, Trivedi R, Maurya R, Singh D, Chattopadhyay N (2013) Greater skeletal gains in ovary intact rats at maturity are achieved by Supplementing a standardized extract of Butea monosperma stem bark that confers better bone conserving effect following ovariectomy and concurrent treatment withdrawal. Evid Based Complement Alternat Med 2013:519387PubMedCentralPubMedGoogle Scholar
- 30.Vasikaran S, Eastell R, Bruyere O, Foldes AJ, Garnero P, Griesmacher A, McClung M, Morris HA, Silverman S, Trenti T, Wahl DA, Cooper C, Kanis JA (2011) Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards. Osteoporos Int 22:391–420PubMedCrossRefGoogle Scholar