Journal of Bone and Mineral Metabolism

, Volume 22, Issue 3, pp 278–282

Bone mass increase specific to the female in a line of transgenic mice overexpressing human osteoblast stimulating factor-1

  • Tamotsu Hashimoto-Gotoh
  • Hideo Ohnishi
  • Atsushi Tsujimura
  • Hiroaki Tsunezuka
  • Kan Imai
  • Haruchika Masuda
  • Toshitaka Nakamura
Short communication

DOI: 10.1007/s00774-003-0485-8

Cite this article as:
Hashimoto-Gotoh, T., Ohnishi, H., Tsujimura, A. et al. J Bone Miner Metab (2004) 22: 278. doi:10.1007/s00774-003-0485-8

Abstract

We have reported that transgenic mice overexpressing human osteoblast stimulating factor-1 (osf1) under the control of the human osteocalcin promoter have a significantly higher bone mineral content and density than nontransgenic littermates. Consequently, bone mass loss due to estrogen deficiency was compensated for in ovariectomized female mice. Here, we show that in this transgenic line, the bone mass increase was evident in female, but not male, mice, as evaluated using the ash assay, double-emission X-ray analysis, and calcein double-labeling to determine the bone formation rate. To elucidate a possible influence on gene expression, we analyzed genomic structures of the inserted transgene and its flanking regions in mouse chromosomes. The results revealed that the transgene was integrated in the mouse repetitive sequences, 234-bp-long Γ-satellite repeats, as inverted multiple (5 + 8) copies. Twelve copies at most seemed to be functional, but no direct evidence supporting female-specific mRNA synthesis of the transgene was obtained.

Key words

OSF1 BDF1 mouse primary hybrid strain littermates bone mass 

Copyright information

© Springer-Verlag Tokyo 2004

Authors and Affiliations

  • Tamotsu Hashimoto-Gotoh
    • 1
    • 2
  • Hideo Ohnishi
    • 3
  • Atsushi Tsujimura
    • 1
    • 2
  • Hiroaki Tsunezuka
    • 2
  • Kan Imai
    • 2
  • Haruchika Masuda
    • 2
  • Toshitaka Nakamura
    • 3
  1. 1.Genomic Medical Sciences, Graduate School of MedicineKyoto Prefectural University of MedicineKyotoJapan
  2. 2.Department of Biochemistry and Molecular Genetics, Research Institute for Neurological Diseases and GeriatricsKyoto Prefectural University of MedicineKyotoJapan
  3. 3.Department of OrthopedicsUniversity of Occupational and Environmental HealthKitakyushuJapan

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