Determination and quality evaluation of Zhilou lotion by quantitative analysis of multicomponent with a single marker

  • Yi Peng
  • Qiong Wang
  • Jing Zou
  • Yuhong Jiang
  • Zhihui LiuEmail author
General Paper


To develop a quantitative analysis of multicomponent by single-marker method for the simultaneous determination of fourteen components in Zhilou lotion, emodin was selected as an internal reference, and the relative correction factors of gallic acid, caffeic acid, polydatin, hyperoside, isoquercitrin, quercitrin, homoplantaginin, resveratrol, hispidulin, aloe emodin, rhein, chrysophanol and emodin methyl ether were established. The accuracy of quantitative analysis of multicomponent by single-marker method was verified by comparing the contents of the fourteen components calculated by the external standard method with those of the quantitative analysis of multicomponent by single-marker method. In a certain linear range, the correction factor of gallic acid, caffeic acid, polydatin, hyperoside, isoquercitrin, quercitrin, homoplantaginin, resveratrol, hispidulin, aloe emodin, rhein, chrysophanol and emodin methyl ether relative to emodin is good. The contents of the 14 components in 10 batches Zhilou lotion were measured. No significant difference was found in the quantitative results between the established quantitative analysis of multicomponent by single-marker method and external standard method. The established quantitative analysis of multicomponent by single-marker method could act as an accurate and convenient method to determine the 14 components in Zhilou lotion and could be used to control the quality of Zhilou lotion.


Quantitative analysis of multicomponents by single marker (QAMS) Zhilou lotion Relative correction factors (RCFs) Emodin 



This work was supported by advantages discipline of Universities in Jiangsu Province (PATA2014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yi Peng
    • 1
  • Qiong Wang
    • 1
  • Jing Zou
    • 1
  • Yuhong Jiang
    • 1
  • Zhihui Liu
    • 1
    Email author
  1. 1.Department of PharmacyNanjing University of Chinese Medicine Affiliated HospitalNanjingChina

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