Accreditation and Quality Assurance

, Volume 23, Issue 4, pp 243–250 | Cite as

Validation of an in-house measurement procedure for determination of selected priority hazardous substances in water and estimation of measurement uncertainty

  • Marijana Kragulj Isakovski
  • Jelena Molnar Jazić
  • Snežana Maletić
  • Aleksandra Tubić
  • Jasmina Agbaba
  • Jelena Tričković
  • Božo DalmacijaEmail author
Practitioner's Report


This study investigates measurement procedure validation for the determination of hexachlorobenzene, pentachlorobenzene and trifluralin as priority hazardous pesticides in water with suspended solids  < 12 mg/L by liquid–liquid extraction with hexane and gas chromatography with electron capture detector analysis. Two water matrices were used: synthetic matrix and a natural matrix—Danube surface water. Limit of detection was in the range 2.2 ng/L–3.9 ng/L. Trueness expressed as recovery was determined at pesticide concentrations of 20 ng/L and 50 ng/L in both water matrices. The intermediate precision was determined as relative standard deviations (RSD) and represents an agreement between replicates. Trueness was 72 %–121 % with RSD < 20 %. Expanded uncertainty of measurement was similar for all compounds (33.3 %–37.5 %) with a 95 % confidence level, for coverage factor k = 2. The validity of the measurement procedure was confirmed by participation in a PT Scheme with satisfactory |z|-score smaller than 2. Based on these results, it can be concluded that this low-cost measurement procedure is fit for the intended use measurements.


Pentachlorobenzene Hexachlorobenzene Trifluralin GC/µECD Validation of measurement procedure 



The project was financed by the kind support of the Provincial Secretariat for Science and Technological Development of the Autonomous Province of Vojvodina (142-451-2452/2018).

Supplementary material

769_2018_1334_MOESM1_ESM.docx (1.1 mb)
Supplementary material 1 (DOCX 1123 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Marijana Kragulj Isakovski
    • 1
  • Jelena Molnar Jazić
    • 1
  • Snežana Maletić
    • 1
  • Aleksandra Tubić
    • 1
  • Jasmina Agbaba
    • 1
  • Jelena Tričković
    • 1
  • Božo Dalmacija
    • 1
    Email author
  1. 1.Department of Chemistry, Biochemistry and Environmental Protection, Faculty of SciencesUniversity of Novi SadNovi SadRepublic of Serbia

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