Accreditation and Quality Assurance

, Volume 12, Issue 9, pp 459–464 | Cite as

An international intercomparison for 19-norandrosterone in human urine: the Comité Consultatif pour la Quantité de Matière (CCQM) Pilot Study CCQM-P68

  • Lindsey G. Mackay
  • Daniel Burke
  • Michael J. Welch
  • Susan Tai
  • Chris Mussell
  • Gavin O’Connor
  • Della Sin
  • Cheng Wing-chi
Practitioner's Report

Abstract

Under the auspices of the Comité Consultatif pour la Quantité de Matière (CCQM) a laboratory intercomparison, CCQM-P68, was co-ordinated by the National Measurement Institute of Australia (NMIA) in 2005 involving the analysis of the steroid 19-norandrosterone (19-NA) in human urine. 19-NA is the major metabolite of nandrolone, a commonly abused anabolic agent. It is routinely tested by many sports drug testing laboratories and several national metrology institutes have developed reference methods to provide reference values for the certification of reference materials. The material distributed for the intercomparison was a freeze-dried human urine candidate reference material containing 19-NA at a level close to the allowed World Anti-Doping Agency (WADA) cut-off for this anabolic agent, i.e. 2 ng/ml. Four national/designated metrology institutes participated in the intercomparison and their results for this complex analysis were in excellent agreement, with a relative standard deviation (RSD) of only 1.7%. The expanded uncertainties at the 95% level of confidence for the reported results ranged from 3.7 to 7.0% at the 2.15 ng/g level.

Keywords

Steroids Nandrolone 19-norandrosterone Urine Laboratory intercomparison Reference method 

Notes

Acknowledgments

The funding by the World Anti-Doping Agency (WADA) for the production of the material used in this pilot study at NMIA is gratefully acknowledged, as is their permission to use the material in this CCQM intercomparison and to publish these results. Funding from the Australian Doping Research Panel is also gratefully acknowledged. The authors would also like to thank the German Sport University, Cologne for providing, via WAADs, a solution of the D4-19-NAG internal standard.

Disclaimer

Certain commercial equipment, instruments, and materials are identified in this paper to adequately specify the experimental procedure. Such identification does not imply recommendation or endorsement by NIST nor does it imply that the equipment, instruments, or materials are necessarily the best available for the purpose.

References

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Lindsey G. Mackay
    • 1
  • Daniel Burke
    • 1
  • Michael J. Welch
    • 2
  • Susan Tai
    • 2
  • Chris Mussell
    • 3
  • Gavin O’Connor
    • 3
  • Della Sin
    • 4
  • Cheng Wing-chi
    • 4
  1. 1.National Measurement Institute, Australia (NMIA)PymbleAustralia
  2. 2.National Institute of Standards and Technology, NISTGaithersburgUSA
  3. 3.LGCTeddingtonUK
  4. 4.Government Laboratory of the Hong Kong Special Administrative Region (GL of HKSAR)Hong KongChina

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