Food safety: screening tests used to detect and quantify GMO proteins
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GMO protein content in maize flours or fresh crops can be easily assessed by the enzyme-linked immunosorbent assays (ELISA) and immunochromatographic (lateral flow) strip tests commercially available. Therefore, ELISA can be seen as a useful tool for screening, for control purposes and for traceability implementation. In order to highlight the importance of monitoring GMO protein presence in food products and to investigate the performance of representative ELISA commercial kits, we evaluated three commercial kits by measuring the amount of Cry1Ab/Cry1Ac in IRMM certified reference materials.
KeywordsGMO Immunoassay ELISA Cry 1Ab
- 2.ISO (2002) : Foodstuffs: methods of analysis for the detection of genetically modified organisms and derived products: protein-based methods. EN ISO 21572:2002, ISO, GenevaGoogle Scholar
- 3.ISO (1994) : Accuracy (trueness and precision) of measurement methods and results. EN ISO 5725:1994, ISO, GenevaGoogle Scholar
- 4.IUPAC (1997) Compendium of chemical terminology; 2nd edn., IUPAC, Research Triangle Park, North CarolinaGoogle Scholar
- 5.Paoletti C, Mazzara M: (2005) Definition of minimum performance requirements for analytical methods of GMO testing. ENGL method performance requirements, EU DG-JRC Ispra, ItalyGoogle Scholar