A sensitive, reliable and rapid high-performance thin-layer chromatography (HPTLC) method for the determination of arctiin and arctigenin in Arctium tomentosum Mill. was established. A. tomentosum Mill. extract was used for chromatographic analysis. The ratio of chloroform and methanol was 48:5 as mobile phase. Temperature is 20–23 °C and humidity is less than 30%. The scanning wavelength is 280 nm. The results showed that arctiin had a good linear relationship in the range of 0.5315–5.8465 μg, r = 0.9982; arctigenin had a good linear relationship in the range of 0.5654–6.2194 μg, r = 0.9951. Precision analysis showed that the RSD < 3.0%. The stability study showed that the sample was stable within 24 h at room temperature, RSD < 2.0%. The average recoveries were 103.07 ± 1.57% and 98.55 ± 2.71%, respectively. The antioxidant activity of Arctium tomentosum Mill. was also identified. The results showed that the antioxidant component identified by thin-layer chromatography–1, 1-diphenyl-2-trinitrophenylhydrazine (TLC-DPPH) was arctigenin not arctiin. The proposed HPTLC is a simple and accurate method for the qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. from different areas.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Xiu L, Dou DQ, Kang YG (2010) The research of Arctium lappa and its changes of the land. Res Pract Chin Med 24:24–26
Repplinger M, Johannesen J, Seitz A (2007) Morphological and molecular evidence for hybridization and introgression in central European Arctium (Asteraceae). Plant Syst Evol 268:75–95
Xu ZH, Tu W, Yang SS, Kang YG (2000) Identification of the Chinese drug great burdock (Arctium lappa) seed and its substitutes by RAPD and TLC. Chin Tradit Herb Drugs 31:57–60
Liu YM (1999) Uygur pharmacology (volume 2). Xinjiang Science and Technology Press, Urumqi, p 132
Wang CT, Zhang XJ, Li FZ, Chen CG (2002) Analysis of fatty acid in Arctium lappa L.seed oil by GC–MS. J Plant Resour Environ 11:58–59
Zhou XY, Ji ZH, Liu L, Bai X (2013) Analysis fatty acid composition of uygur medicine Arctium tomentosum Mill. seeds by GC–MS. J Xinjiang Med Univ 36:464–466 +471
Ul'chenko NT, Gigienova EI, Umarov AU (1981) Hydroxy acids of the seed oils of five plants of the family Asteraceae. Chem Nat Compd 17:26–30
Zhang HK, Ge L, Tian SG (2010) Study on antioxidant activity of ethanol extracts from Arctium tomentosum Mill. Northwest Pharm J 25:346–348
Wang Z, Li P, Wang CJ (2015) Protective effects of Arctium lappa L. root extracts (AREs) on high fat diet induced quail atherosclerosis. BMC Complem Altern Med 16:5–10
Huang TC, Tsai SS (2010) Effect of Arctium lappa L. in the dextran sulfate sodium colitis mouse model. World J Gastroenterol 16:4193–4199
Ji KY, Jang JH, Lee EH (2018) Canavalia gladiata and Arctium lappa extracts ameliorate dextran sulphate sodium-induced inflammatory bowel disease by enhancing immune responses. J Funct Foods 45:24–33
Sun J, Wang XY, Zang N, Shi CL, Li Q, Chen D (2008) Antifungal activity in vitro of Arctium ethanol extract. J Henan Agric Sci 37:74–77
Hirose M, Yamaguchi T, Lin C (2000) Effects of arctiin of PhIP-induced mammary, colon and pancreatic carcinogenesisin female Sprague-Dawleyrats and MeIQx-induced hepato-carcinogenesis in male F344 rats. Cancer Lett 155:79–88
Hausott B, Greger H, Marian B (2003) Naturally occurringlignans efficiently induce apoptosis in colorectal tumor cells. J Cancer Res Clin Oncol 129:569–576
Hirano T, Gotoh M, Oka K (1994) Natural flavonoids and lignans are potent cytostatic agents against human leukemic HL-60 cells. Life Sci 55:1061–1069
Luo XZ, Wen E, Tian SG, Zhou XY (2016) Study on screening of effective parts from Arctium tomentosum Mill. seeds by antioxidant and hypoglycemic activity. Mod Chin Med 18:1265–1268
Gao Y, Dong X, Kang YG, Zhao CZ, Huang Z, Zhang XY (2002) Activity of in vitro anti-influenza virus of arctigenin. Chin Tradit Herb Drugs 33:54–56
Zhang B, Wang B, Wang YQ, Cao SH (2014) Hpyerglycemic effect of arctiin in db/db mice with spontaneous diabetes. China Pharm 17:1796–1799
Shihara K, Yamagishi N, Saito Y (2006) Arctigenin from Fructusn Arctii is a novel suppressor of heat shock responsein mammalian cells. Cell Stress Chaperones 11:154–161
Lee JY, Kim CJ (2010) Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan, inhibits type I–IV allergic inflammation and pro-inflammatory enzymes. Arch Pharm Res 33:947–957
Liu RN, Liu WJ, Qiu JG, Luo HY (2018) Proliferation inhibition effect of arctigenin on endometrial carcinoma HEC-1B cell and its mechanism. World Chin Med 13:2015–2018
Xiang JL, Wang W, Ren FY (2018) Application of thin layer chromatography in the examination of traditional Chinese medicine. Guide China Med 16:237–238
Wang TN, Zhang H, Chen YS, Xu XM, Huang CH (2019) Reliable screening of rutin in healthcare products by HPTLC–DPPH assay. Food Mach 35:92–96 +142
Financial support of this work was from the Xinjiang Science and Technology Fund (Grant numbers 2015211C025).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ge, L., Liu, F., Hu, Y. et al. Qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. by high-performance thin-layer chromatography. JPC-J Planar Chromat (2020). https://doi.org/10.1007/s00764-019-00005-z
- Arctium tomentosum Mill.
- High-performance thin-layer chromatography