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Qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. by high-performance thin-layer chromatography


A sensitive, reliable and rapid high-performance thin-layer chromatography (HPTLC) method for the determination of arctiin and arctigenin in Arctium tomentosum Mill. was established. A. tomentosum Mill. extract was used for chromatographic analysis. The ratio of chloroform and methanol was 48:5 as mobile phase. Temperature is 20–23 °C and humidity is less than 30%. The scanning wavelength is 280 nm. The results showed that arctiin had a good linear relationship in the range of 0.5315–5.8465 μg, r = 0.9982; arctigenin had a good linear relationship in the range of 0.5654–6.2194 μg, r = 0.9951. Precision analysis showed that the RSD < 3.0%. The stability study showed that the sample was stable within 24 h at room temperature, RSD < 2.0%. The average recoveries were 103.07 ± 1.57% and 98.55 ± 2.71%, respectively. The antioxidant activity of Arctium tomentosum Mill. was also identified. The results showed that the antioxidant component identified by thin-layer chromatography–1, 1-diphenyl-2-trinitrophenylhydrazine (TLC-DPPH) was arctigenin not arctiin. The proposed HPTLC is a simple and accurate method for the qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. from different areas.

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Financial support of this work was from the Xinjiang Science and Technology Fund (Grant numbers 2015211C025).

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Correspondence to Xiaoying Zhou.

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Ge, L., Liu, F., Hu, Y. et al. Qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. by high-performance thin-layer chromatography. JPC-J Planar Chromat (2020).

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  • Arctium tomentosum Mill.
  • High-performance thin-layer chromatography
  • Arctiin
  • Arctigenin