Biotechnological applications of nanostructured hybrids of polyamine carbon quantum dots and iron oxide nanoparticles
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The combination of different nanomaterials has been investigated during the past few decades and represents an exciting challenge for the unexpected emerging properties of the resulting nano-hybrids. Spermidine (Spd), a biogenic polyamine, has emerged as a useful functional monomer for the development of carbon quantum dots (CQDs). Herein, an electrostatically stabilized ternary hybrid, constituted of iron oxide-DNA (the core) and spermidine carbon quantum dots (CQDSpds, the shell), was self-assembled and fully characterized. The as-obtained nano-hybrid was tested on HeLa cells to evaluate its biocompatibility as well as cellular uptake. Most importantly, besides being endowed by the magnetic features of the core, it displayed drastically enhanced fluorescence properties in comparison with parent CQDSpds and it is efficiently internalized by HeLa cells. This novel ternary nano-hybrid with multifaceted properties, ranging from fluorescence to superparamagnetism, represents an interesting option for cell tracking.
KeywordsSpermidine Carbon quantum dots Nano-hybrid Cell tracker Magnetic nanoparticle
Carbon quantum dot
Energy-dispersive X-ray spectroscopy
Fourier Transform Infrared Spectroscopy
Surface active maghemite nanoparticles
Salmon sperm deoxyribonucleic acid
Transmission electron microscopy
The present experimental work was partially funded by Italian Institutional Ministry Grants Cod. DOR1872491. The team members from the Czech Republic were supported by Grant No. LO1204 from the Ministry of Education, Youth and Sports. The authors thank Dr. Jana Stráská for TEM measurements. The authors also thank ‘La Sapienza’ University of Rome and Italian MIUR (Ministero dell’Istruzione, dell’Università e della Ricerca). Our gratitude is also due to the “International Polyamine Foundation–ONLUS” for the availability to look up in the Polyamines documentation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and animal rights
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bulte JWM, Arbab AS, Douglas T, Frank JA (2004) Preparation of magnetically labeled cells for cell tracking by magnetic resonance imaging. Methods in Enzymology. Academic Press, Cambridge, pp 275–299Google Scholar
- Kittel C (2004) Introduction to solid state physics, 8th edn. Wiley, New YorkGoogle Scholar
- Magro M, Nodari L, Russo U, et al (2012b) Maghemite nanoparticles and method for preparing thereof. International Patent Application WO2012/010200 A1; US 8,980, 218 B2Google Scholar
- Shipway AN, Katz E, Willner I (2000) Nanoparticle arrays on surfaces for electronic, optical, and sensor applications. ChemPhysChem 1:18–52. https://doi.org/10.1002/1439-7641(20000804)1:1%3c18:AID-CPHC18%3e3.0.CO;2-L CrossRefGoogle Scholar
- Stroh A, Zimmer C, Gutzeit C et al (2004) Iron oxide particles for molecular magnetic resonance imaging cause transient oxidative stress in rat macrophages. Free Radic Biol Med 36:976–984. https://doi.org/10.1016/j.freeradbiomed.2004.01.016 CrossRefGoogle Scholar
- Xia Y, Gates B, Li Z-Y (2001) Self-assembly approaches to three-dimensional photonic crystals. Adv Mater 13:409–413. https://doi.org/10.1002/1521-4095(200103)13:6%3c409:AID-ADMA409%3e3.0.CO;2-C CrossRefGoogle Scholar
- Zayats M, Kharitonov AB, Pogorelova SP et al (2003) Probing photoelectrochemical processes in Au-CdS nanoparticle arrays by surface plasmon resonance: application for the detection of acetylcholine esterase inhibitors. J Am Chem Soc 125:16006–16014. https://doi.org/10.1021/ja0379215 CrossRefGoogle Scholar