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Amino Acids

pp 1–11 | Cite as

Glycine supplementation to breast-fed piglets attenuates post-weaning jejunal epithelial apoptosis: a functional role of CHOP signaling

  • Xiaoxiao Fan
  • Shuai Li
  • Zhenlong WuEmail author
  • Zhaolai Dai
  • Ju Li
  • Xiaolong Wang
  • Guoyao Wu
Original Article

Abstract

This study was conducted to test the hypothesis that preweaning  glycine supplementation to breast-fed piglets alleviated the post-weaning  apoptosis of jejunal epithelium through CHOP signaling. Seven-day-old sow-reared piglets were orally administrated with 0, 50, 100, or 200% of glycine intake from sow’s milk twice daily for 14 days and then were weaned at 21 days of age. Tissue samples were collected at 28 days of age for determining intestinal morphology, serum diamine oxidase (DAO) activity, abundances of proteins involved in ER stress and apoptosis. Glycine (100–200%) administration increased villus height, the ratio of villus height to crypt depth in the jejunum. Glycine supplementation (200%) enhanced average daily weight gain during the first 2 weeks post-weaning. Serum DAO activity and jejunal epithelium apoptosis were decreased, but the number of goblet cells in the jejunum was increased. Western blot analysis showed that 100–200% glycine enhanced the protein levels of occludin, claudin-1, and zonula occludens (ZO)-1 without affecting those of claudin-3, ZO-2, and ZO-3. Further studies showed that protein abundances of glucose-regulated protein 78 (BiP/GRP78) and p-IRE1α, instead of ATF6α, were reduced by glycine. Among the proteins related to apoptosis, abundances of CHOP and p53 were reduced, whereas those of Bcl-2 and Bcl-xL were enhanced in the jejunum of 100–200% glycine-supplemented piglets. Collectively, our results indicated that preweaning glycine supplementation improved the intestinal development of post-weaning piglets. The beneficial effect of glycine was associated with improved intestinal mucosal barrier and reduced apoptosis of enterocytes through CHOP signaling.

Keywords

Glycine Endoplasmic reticulum stress Small intestine Apoptosis Piglets 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31572412, 31572410, 31625025, 31272451 and  31272450), the Zhengzhou 1125 Talent Program, Agriculture and Food Research Initiative Competitive Grants (2014-67015-21770, 2015-67015-23276 and 2016-67015-24958) from the USDA National Institute of Food and Agriculture, and Texas A&M AgriLife Research (H-8200).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics statement

The studies were approved by China Agricultural University Institutional Animal Science and Technology College and conducted according to the Guidelines for Experimental Animal Research of the Ministry of Science and Technology (Beijing, China).

Informed consent

All authors have read and approved the final manuscript.

Supplementary material

726_2018_2681_MOESM1_ESM.docx (13 kb)
Supplementary material 1 (DOCX 14 KB)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoxiao Fan
    • 1
    • 2
  • Shuai Li
    • 2
  • Zhenlong Wu
    • 1
    • 2
    Email author
  • Zhaolai Dai
    • 3
  • Ju Li
    • 2
  • Xiaolong Wang
    • 3
  • Guoyao Wu
    • 2
    • 4
  1. 1.Beijing Advanced Innovation Center for Food Nutrition and Human HealthChina Agricultural UniversityBeijingChina
  2. 2.Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal NutritionChina Agricultural UniversityBeijingChina
  3. 3.Henan Yinfa Animal Husbandry CoXinzhengChina
  4. 4.Department of Animal ScienceTexas A&M UniversityCollege StationUSA

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