An insecticidal toxin from Nephila clavata spider venom
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Spiders are the most successful insect predators given that they use their venom containing insecticidal peptides as biochemical weapons for preying. Due to the high specificity and potency of peptidic toxins, discoveries of insecticidal toxins from spider venom have provided an opportunity to obtain natural compounds for agricultural applications without affecting human health. In this study, a novel insecticidal toxin (μ-NPTX-Nc1a) was identified and characterized from the venom of Nephila clavata. Its primary sequence is GCNPDCTGIQCGWPRCPGGQNPVMDKCVSCCPFCPPKSAQG which was determined by automated Edman degradation, cDNA cloning, and MS/MS analysis. BLAST search indicated that Nc1a shows no similarity with known peptides or proteins, indicating that Nc1a belongs to a novel family of insecticidal peptide. Nc1a displayed inhibitory effects on NaV and KV channels in cockroach dorsal unpaired median neurons. The median lethal dose (LD50) of Nc1a on cockroach was 573 ng/g. Herein, a study that identifies a novel insecticidal toxin, which can be a potential candidate and/or template for the development of bioinsecticides, is presented.
KeywordsToxin Spider Venom Nephila clavata Bioinsecticide
We thank Dr. Zeng Lin for technical advice and assistance on MS/MS spectra analysis. This work was supported by funding from the Ministry of Science and Technology of China (2013CB911304), National Science Foundation of China (331372208 and U1302221), Chinese Academy of Sciences (XDA12040209, QYZDJ-SSW-SMC012), Science and technology office of Jiangsu Province (BE2016742) and Yunnan Province (2015HA023) to R.L., National Science Foundation of China (31640071) and Chinese Academy of Sciences (XDA12020334 and Youth Innovation Promotion Association) to S.Y., and National Science Foundation of China (31201717), Jiangsu Province (Q0201600440) to X.W.Y.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Informed consent was obtained from all individual participants included in the study.
- Bende NS, Dziemborowicz S, Mobli M, Herzig V, Gilchrist J, Wagner J, Nicholson GM, King GF, Bosmans F (2014) A distinct sodium channel voltage-sensor locus determines insect selectivity of the spider toxin Dc1a. Nat Commun 11(5):4350Google Scholar
- Bende NS, Dziemborowicz S, Herzig V, Ramanujam V, Brown GW, Bosmans F, Nicholson GM, King GF, Mobli M (2015) The insecticidal spider toxin sfi1 is a knottin peptide that blocks the pore of insect voltage-gated sodium channels via a large beta-hairpin loop. The FEBS J 282:904–920CrossRefPubMedGoogle Scholar
- Borges CR, Sherma ND (2014) Techniques for the analysis of cysteine sulfhydryls and oxidative protein folding. Antioxid Redox Signal 21(3):511–531Google Scholar
- Herzig V, Ikonomopoulou M, Smith JJ, Dziemborowicz S, Gilchrist J, Kuhn-Nentwig L, Rezende FO, Moreira LA, Nicholson GM, Bosmans F, King GF (2016) Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami. Sci Rep 7(6):29538CrossRefGoogle Scholar
- Hisada M, Fujita T, Naoki H, Itagaki Y, Irie H, Miyashita M, Nakajima T (1998) Structures of spider toxins: hydroxyindole-3-acetylpolyamines and a new generalized structure of type-e compounds obtained from the venom of the joro spider, Nephila clavata. Toxicon 36:1115–1125CrossRefPubMedGoogle Scholar
- Højland DH, Nauen R, Foster SP, Williamson MS, Kristensen M (2015) Incidence, spread and mechanisms of pyrethroid resistance in european populations of the cabbage stem flea beetle, Psylliodes chrysocephala L. (Coleoptera: Chrysomelidae). PLoS One 10(12):e0146045Google Scholar
- Jones CM, Haji KA, Khatib BO, Bagi J, Mcha J, Devine GJ, Daley M, Kabula B, Ali AS, Majambere S, Ranson H (2013) The dynamics of pyrethroid resistance in Anopheles arabiensis from Zanzibar and an assessment of the underlying genetic basis. Parasit Vectors 6(6):343CrossRefPubMedPubMedCentralGoogle Scholar
- Liu J, Jiang J, Wu Z, Xie F (2012) Antimicrobial peptides from the skin of the Asian frog, Odorrana jingdongensis: de novo sequencing and analysis of tandem mass spectrometry data. J Proteom 75(18):5807–5821Google Scholar
- Mourao CB, Heghinian MD, Barbosa EA, Mari F, Bloch CJ, Restano-Cassulini R, Possani LD, Schwartz EF (2013) Characterization of a novel peptide toxin from Acanthoscurria paulensis spider venom: a distinct cysteine assignment to the HWTX-II family. Biochemistry 52:2440–2452CrossRefPubMedGoogle Scholar
- Oliveira LC, Campos FV, Figueiredo SG, Cordeiro MN, Adaime BR, Richardson M, Pimenta AM, Martin-Eauclaire MF, Beirao PS, De Lima ME (2015) Beta/delta-prit1, a highly insecticidal toxin from the venom of the brazilian spider Phoneutria reidyi (f.O. Pickard-cambridge, 1897). Toxicon 104:73–82. doi: 10.1016/j.toxicon.2015.07.010 CrossRefPubMedGoogle Scholar