An insecticidal toxin from Nephila clavata spider venom
Spiders are the most successful insect predators given that they use their venom containing insecticidal peptides as biochemical weapons for preying. Due to the high specificity and potency of peptidic toxins, discoveries of insecticidal toxins from spider venom have provided an opportunity to obtain natural compounds for agricultural applications without affecting human health. In this study, a novel insecticidal toxin (μ-NPTX-Nc1a) was identified and characterized from the venom of Nephila clavata. Its primary sequence is GCNPDCTGIQCGWPRCPGGQNPVMDKCVSCCPFCPPKSAQG which was determined by automated Edman degradation, cDNA cloning, and MS/MS analysis. BLAST search indicated that Nc1a shows no similarity with known peptides or proteins, indicating that Nc1a belongs to a novel family of insecticidal peptide. Nc1a displayed inhibitory effects on NaV and KV channels in cockroach dorsal unpaired median neurons. The median lethal dose (LD50) of Nc1a on cockroach was 573 ng/g. Herein, a study that identifies a novel insecticidal toxin, which can be a potential candidate and/or template for the development of bioinsecticides, is presented.
KeywordsToxin Spider Venom Nephila clavata Bioinsecticide
We thank Dr. Zeng Lin for technical advice and assistance on MS/MS spectra analysis. This work was supported by funding from the Ministry of Science and Technology of China (2013CB911304), National Science Foundation of China (331372208 and U1302221), Chinese Academy of Sciences (XDA12040209, QYZDJ-SSW-SMC012), Science and technology office of Jiangsu Province (BE2016742) and Yunnan Province (2015HA023) to R.L., National Science Foundation of China (31640071) and Chinese Academy of Sciences (XDA12020334 and Youth Innovation Promotion Association) to S.Y., and National Science Foundation of China (31201717), Jiangsu Province (Q0201600440) to X.W.Y.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Informed consent was obtained from all individual participants included in the study.
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