Amino Acids

, Volume 49, Issue 6, pp 1029–1039 | Cite as

The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation

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Abstract

Cell culture and cryopreservation are necessary for clinical therapy and cells storage. The addition of 10% (v/v) foetal bovine serum (FBS) to basal culture media has been common practice and is one of the most widely used methods. FBS media added with 10% DMSO (dimethyl sulfoxide) have also been used for cryopreservation cells. Ideally, FBS should be avoided because of high cost and bio-safety. Silk sericin has been used as a serum substitute and an additive due to its good hydrophilicity and biological safety. This article summarizes a few details about the processing of sericin and its application as a serum substitute or an additive for cell culture and cryopreservation media. Sericin can be a potential novel serum substitute or an additive for cell culture and cryopreservation media.

Keywords

Sericin Recovery Serum Cell culture Cryopreservation 

Abbreviations

FBS

Foetal bovine serum

DMSO

Dimethyl sulfoxide

BSA

Bovine serum albumin

B. mori

Bombyx mori

SFM

Serum-free media

TIDM

Type I diabetes mice

ROS

Reactive oxygen species

bcl-2

B-cell lymphoma 2

E. coli

Escherichia coli

BW

Body weight

COCs

Cumulus-oocyte complexes

IVM

In vitro maturation

ASCs

Adipose tissue-derived stem/progenitor cells

MSCs

Marrow stromal cells

HOST

Hypo-osmotic swelling

GPx

Glutathione peroxidase

SOD

Superoxide dismutase

MDA

Malondialdehyde

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Notes

Acknowledgements

The authors gratefully acknowledge the earmarked fund (CARS-22-ZJ0504) for the China Agriculture Research System (CARS) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, P. R. China.

Compliance with ethical standards

Conflict of interest

The authors declared that they have no conflicts of interest.

The manuscript is a mini-review, and it does not involved in the ethical issues.

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Biotechnology LaboratorySchool of Biology and Basic Medical Sciences, Soochow UniversitySuzhouPeople’s Republic of China

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