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Amino Acids

, Volume 48, Issue 9, pp 2131–2144 | Cite as

Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs

  • Yehui Duan
  • Yangmiao Duan
  • Fengna LiEmail author
  • Yinghui Li
  • Qiuping Guo
  • Yujiao Ji
  • Bie Tan
  • Tiejun Li
  • Yulong YinEmail author
Original Article

Abstract

Branched-chain amino acids (BCAA), including leucine (Leu), isoleucine (Ile), and valine (Val), play critical roles in energy homeostasis and lipid metabolism in addition to their other functions, such as in protein metabolism. This study investigated the effects of different dietary BCAA ratios on the intramuscular fat (IMF) content and fatty acid composition in different location of skeletal muscles, including the longissimus dorsi (LD), biceps femoris (BF), and psoas major (PM) muscles of growing pigs, and also examined the mRNA expression levels of genes involved in lipid metabolism in these muscle tissues. The experiment was performed on 40 growing pigs (Large White × Landrace) with a similar initial weight (9.85 ± 0.35 kg). The pigs were randomly assigned to one of five diets: diet A was a positive control and contained 20 % crude protein (CP) with a Leu:Ile:Val ratio of 1:0.51:0.63 according to the recommendation of the National Research Council (NRC); for diets B to E, the CP level was reduced to 17 %, and the Leu:Ile:Val ratios were 1:1:1, 1:0.75:0.75, 1:0.51:0.63, and 1:0.25:0.25, respectively. No significant difference was observed in the average feed intake and feed efficiency of the pigs fed the low protein diet (17 % CP) with BCAA treatments relative to the positive control. However, there was a tendency for increased feed efficiency of the 1:0.75:0.75 group compared with the 1:1:1 group (P = 0.09). The BCAA ratio of 1:0.75:0.75 (17 % CP) increased the IMF content of BF muscle (P < 0.01). Moreover, varied dietary BCAA supplementation with a reduced protein level had different effects on the fatty acid composition of the LD, BF, and PM muscles. The BCAA ratio of 1:0.51:0.63–1:0.75:0.75 (17 % CP) significantly lowered the ratio of n-6 to n-3 polyunsaturated fatty acid in these muscles compared with the positive control group (20 % CP). This effect was associated with an increase in mRNA expression levels of acetyl-CoA carboxylase, lipoprotein lipase, fatty acid transport protein, and fatty acid binding protein 4 in the muscles (P < 0.05). The results indicated that the reduced protein diet (17 % CP) with the BCAA ratio within 1:0.25:0.25–1:0.75:0.75 could increase the IMF content in BF muscle and significantly improve the fatty acid composition in different skeletal muscles accompanied by changes in the expression of genes involved in lipid metabolism, compared with those in the pigs that received adequate dietary protein (20 %), which might result in improved eating quality and nutritional value of the meat.

Keywords

Branched-chain amino acid ratio Fatty acid composition Lipid metabolism Skeletal muscles Growing pigs 

Abbreviations

ACC

Acetyl-CoA carboxylase

ADG

Average daily gain

ADFI

Average daily feed intake

BCAA

Branched-chain amino acids

BF

Biceps femoris

CHD

Coronary heart disease

CP

Crude protein

FABP4

Fatty acid binding protein 4

FATP-1

Fatty acid transport protein

HDL-C

High density lipoprotein-cholesterol

HSL

Hormone-sensitive lipase

Ile

Isoleucine

IMF

Intramuscular fat

LD

Longissimus dorsi

LDL-C

Low density lipoprotein-cholesterol

Leu

Leucine

LPL

Lipoprotein lipase

LPS

Lipase

M-CPT-1

Muscle carnitine palmitoyltransferase 1

MUFA

Monounsaturated fatty acid

NEFAs

Nonesterified fatty acids

NRC

National Research Council

PM

Psoas major

PUFA

Polyunsaturated fatty acid

SFA

Saturated fatty acid

TAG

Triacylglycerol

TC

Total cholesterol

Val

Valine

Notes

Acknowledgments

This study was jointly supported by National Basic Research Program of China (2013CB127305, 2012CB124704), National Nature Science Foundation of China (31110103909, 31330075), Youth Innovation Promotion Association CAS (2015), Nature Science Foundation of Hunan Province (2015JJ2146), and The Chinese Academy of Science STS Project (KFJ-EW-STS-063).

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

Ethical standards

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted. This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Yehui Duan
    • 1
    • 2
  • Yangmiao Duan
    • 2
    • 3
  • Fengna Li
    • 1
    • 4
    Email author
  • Yinghui Li
    • 1
    • 2
  • Qiuping Guo
    • 1
    • 2
  • Yujiao Ji
    • 1
  • Bie Tan
    • 1
    • 4
    • 5
  • Tiejun Li
    • 1
  • Yulong Yin
    • 1
    • 5
    • 6
    Email author
  1. 1.Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, Research Center for Healthy Breeding Livestock and Poultry, Hunan Engineering and Research Center for Animal and Poultry Science, Institute of Subtropical Agriculture, Ministry of AgricultureChinese Academy of ScienceChangshaChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.Beijing Institute of GenomicsChinese Academy of SciencesBeijingChina
  4. 4.Hunan Co-Innovation Center of Animal Production Safety (CICAPS)ChangshaChina
  5. 5.Hunan Collaborative Innovation Center for Utilization of Botanical Functional IngredientsChangshaChina
  6. 6.School of BiologyHunan Normal UniversityChangshaChina

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