Amino Acids

, Volume 48, Issue 2, pp 499–504 | Cite as

Implications of a pre-exercise alkalosis-mediated attenuation of HSP72 on its response to a subsequent bout of exercise

  • Daniel J. PeartEmail author
  • Richard J. Kirk
  • Leigh A. Madden
  • Rebecca V. Vince
Original Article


The aim of this study was to investigate if a pre-exercise alkalosis-mediated attenuation of HSP72 had any effect on the response of the same stress protein after a subsequent exercise. Seven physically active males [25.0 ± 6.5 years, 182.1 ± 6.0 cm, 74.0 ± 8.3 kg, peak aerobic power (PPO) 316 ± 46 W] performed a repeated sprint exercise (EXB1) following a dose of 0.3 g kg−1 body mass of sodium bicarbonate (BICARB), or a placebo of 0.045 g kg−1 body mass of sodium chloride (PLAC). Participants then completed a 90-min intermittent cycling protocol (EXB2). Monocyte expressed HSP72 was significantly attenuated after EXB1 in BICARB compared to PLAC, however, there was no difference in the HSP72 response to the subsequent EXB2 between conditions. Furthermore there was no difference between conditions for measures of oxidative stress (protein carbonyl and HSP32). These findings confirm the sensitivity of the HSP72 response to exercise-induced changes in acid–base status in vivo, but suggest that the attenuated response has little effect upon subsequent stress in the same day.


HSP72 Bicarbonate Alkalosis Stress Monocyte 



The authors wish to thank those who volunteered for the study, and declare that the research project was internally funded.

Compliance with ethical standards

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Daniel J. Peart
    • 1
    • 2
    Email author
  • Richard J. Kirk
    • 1
  • Leigh A. Madden
    • 3
  • Rebecca V. Vince
    • 1
  1. 1.Department of Sport, Health and Exercise ScienceUniversity of HullHullUK
  2. 2.Sport and BiosciencesUniversity Centre, North Lindsey CollegeScunthorpeUK
  3. 3.Centre for Biomedical ResearchUniversity of HullHullUK

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