Amino Acids

, Volume 46, Issue 2, pp 353–366 | Cite as

Abomasal infusion of arginine stimulates SCD and C/EBPß gene expression, and decreases CPT1ß gene expression in bovine adipose tissue independent of conjugated linoleic acid

  • Seong Ho Choi
  • Tryon A. Wickersham
  • Guoyao Wu
  • L. Anne Gilmore
  • Holly D. Edwards
  • Sung Kwon Park
  • Kyoung Hoon Kim
  • Stephen B. Smith
Original Article

Abstract

Based on previous research with bovine peadipocytes, we hypothesized that infusion of arginine into the abomasum of Angus steers stimulates stearoyl-CoA desaturase (SCD) gene expression in bovine subcutaneous (s.c.) adipose tissue, and that this would be attenuated by conjugated linoleic acid (CLA). Growing Angus steers were infused abomasally with l-arginine 50 g/day; n = 13; provided as l-arginine HCl) or l-alanine (isonitrogenous control, 100 g/day; n = 11) for 14 days. For the subsequent 14 days, half of the steers in each amino acid group were infused with CLA (100 g/day). Body weight gain and average daily gain were unaffected (P > 0.15) by infusion of arginine or CLA into the abomasum. The plasma concentrations of cis-9, trans-11 and trans-10, cis-12 CLA were increased CLA infusion (P = 0.001) and infusion of arginine increased plasma arginine (P = 0.01). Compared with day 0, fatty acid synthase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase enzyme activities in s.c. adipose tissue increased by day 14 in steers infused with either alanine or arginine (all P < 0.01). NADP-MDH activity was higher (P = 0.01) in steers infused with arginine than in steers infused with arginine plus CLA by day 28, but lipid synthesis in vitro from glucose and acetate was unaffected by infusion of either arginine or CLA (P > 0.40). By day 28, C/EBPβ and SCD gene expression was higher, and CPT1β gene expression was lower, in s.c. adipose tissue of steers infused with arginine than in steers infused with alanine (±CLA) (P = 0.05). CLA decreased adipose tissue oleic acid (18:1n-9) in alanine- or arginine-infused steers (P = 0.05), although CLA had no effect on SCD gene expression. The data indicate that supplemental arginine promotes adipogenic gene expression and may promote lipid accumulation in bovine adipose tissue. l-Arginine may beneficially improve beef quality for human consumption.

Keywords

Adipose tissue Adipogenesis Arginine Beef cattle Conjugated linoleic acid 

Abbreviations

AMPKα

AMP-activated protein kinase-α

C/EBPβ

CCAAT/enhancer-binding protein-β

CLA

Conjugated linoleic acid

CPT1β

Carnitine palmitoyltransferase-1ß

FAME

Fatty acid methyl esters

FAS

Fatty acid synthase

G6PDH

Glucose-6-phosphate dehydrogenase

GPR43

G-coupled protein receptor-43

NADP-MDH

NADP-malate dehydrogenase

6PGDH

6-Phosphogluconate dehydrogenase

PPARγ

Peroxisome proliferator-activated receptor-γ

qPCR

Quantitative real-time polymerase chain reaction

RPS9

40S ribosomal protein S9

SCD

Stearoyl-coenzyme A desaturase

s.c.

Subcutaneous

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Seong Ho Choi
    • 2
  • Tryon A. Wickersham
    • 1
  • Guoyao Wu
    • 1
  • L. Anne Gilmore
    • 1
  • Holly D. Edwards
    • 1
  • Sung Kwon Park
    • 3
  • Kyoung Hoon Kim
    • 3
  • Stephen B. Smith
    • 1
  1. 1.Department of Animal Science, 2471 TAMUTexas A&M UniversityCollege StationUSA
  2. 2.Department of Animal ScienceChungbuk National UniversityCheongjuRepublic of Korea
  3. 3.National Institute of Animal ScienceRural Development AdministrationSuwonRepublic of Korea

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