Amino Acids

, Volume 45, Issue 4, pp 877–887 | Cite as

Metabolomic analysis of amino acid metabolism in colitic rats supplemented with lactosucrose

  • Zheng Ruan
  • Yinfei Lv
  • Xiaofang Fu
  • Qinghua He
  • Zeyuan Deng
  • Wenqun Liu
  • Yu Yingli
  • Xiaosong Wu
  • Guoyao Wu
  • Xin Wu
  • Yulong Yin
Original Article

Abstract

Intestinal inflammation causes metabolic disorders. The purpose of this study was to determine the effect of dietary supplementation with lactosucrose (LS) on the serum metabolome and intestinal luminal content of fatty acids in colitic rats. Colitis was induced in rats using trinitrobenzene sulfonic acid. Subsequently, rats received intragastric administration of either 250 mg LS/kg body weight or saline (the control group) every day for 5 weeks. Short-chain fatty acids in the intestinal lumen, blood profile, and metabolites in serum were measured, respectively, using gas chromatography, biochemistry analyzer, and nuclear magnetic resonance-based metabolomics combined with multivariate statistics. Metabolic effects of LS included: (1) decreases in concentrations of branched-chain amino acids (isoleucine and valine), alanine, citric acid, trimethylamine oxide and taurine, and the abundance of aspartate aminotransferase in serum; (2) increases in concentrations of glucose metabolites (including succinate) in serum; and (3) altered concentrations of butyrate in the cecal content and of butyrate and acetate in the colon content. The results indicate that LS supplementation to colitic rats affects whole-body metabolism of amino acids and release of aspartate aminotransferase and alkaline phosphatase from tissues into the blood circulation, and enhances the production of short-chain fatty acids in the intestinal lumen.

Keywords

Amino acids Metabolites Lactosucrose Inflammation Nuclear magnetic resonance spectroscopy 

Abbreviations

CMDI

Colonic mucosal damage index

IBD

Inflammatory bowel disease

LS

Lactosucrose

NMR

Nuclear magnetic resonance

PCA

Principal component analysis

SCFA

Short-chain fatty acids

TCA

Tricarboxylic acid

TNBS

Trinitrobenzene sulfonic acid

Notes

Acknowledgments

We are grateful to the National Natural Science Foundation of China (Grant No. 31001014), and the China Postdoctoral Science Foundation funded project (200902537). This research was supported by Intestinal Barrier Foundation of Li Jieshou Academician of CAS (LJS_201006), the research program of State Key Laboratory of Food Science and Technology, Nanchang University (No. SKLF-QN-201110), Chinese Academy of Science Project for XinJiang and Henan CXJQ20113; National Natural Science Foundation of China (No. 31110103909,30901040, 30928018, and 31101729); and Texas A&M AgriLife Research Hatch Project (H-8200).

Conflict of interest

The authors declare no conflict of interest.

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Zheng Ruan
    • 1
  • Yinfei Lv
    • 1
  • Xiaofang Fu
    • 2
  • Qinghua He
    • 3
  • Zeyuan Deng
    • 1
  • Wenqun Liu
    • 1
  • Yu Yingli
    • 1
  • Xiaosong Wu
    • 4
  • Guoyao Wu
    • 5
    • 6
  • Xin Wu
    • 1
    • 7
  • Yulong Yin
    • 1
    • 7
  1. 1.State Key Laboratory of Food Science and Technology, College of Life Science and Food EngineeringNanchang UniversityNanchangChina
  2. 2.Analytic Center for Hubei Entry-exit Inspection and Quarantine BureauWuhanChina
  3. 3.Department of Food Science and Engineering, College of Chemistry and Chemical EngineeringShenzhen UniversityShenzhenChina
  4. 4.College of Animal Science and TechnologyHunan Agricultural UniversityChangshaChina
  5. 5.State Key Laboratory of Animal Nutrition, College of Animal Science and TechnologyChina Agricultural UniversityBeijingChina
  6. 6.Department of Animal Science, Faculty of NutritionTexas A&M UniversityCollege StationUSA
  7. 7.Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical AgricultureChinese Academy of SciencesChangshaChina

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