Advertisement

Amino Acids

, Volume 45, Issue 4, pp 811–819 | Cite as

Taurine prevented cell cycle arrest and restored neurotrophic gene expression in arsenite-treated SH-SY5Y cells

  • Chien-Te Chou
  • Wen-Feng Lin
  • Zwe-Ling Kong
  • Shiow-Yi Chen
  • Deng-Fwu HwangEmail author
Original Article

Abstract

The study investigated the effect of taurine on cell viability and neurotrophic gene expression in arsenite-treated human neuroblastoma SH-SY5Y cells. Arsenite-induced intracellular reactive oxygen species (ROS) and interrupted cell cycle in SH-SY5Y cells. In addition, arsenite reduced mitochondria membrane potential (MMP) and decreased neurotrophic gene expressions such as n-myc downstream-regulated gene 4 (NDRG-4), brain-derived neurotrophic factor (BDNF) and sirtuin-1 (SIRT-1) in SH-SY5Y cells. In parallel, taurine prevented cell cycle, restored MMP and reduced the intracellular ROS level, and taurine recovered NDRG-4, BDNF and SIRT-1 gene expressions in arsenite-treated SH-SY5Y cells while taurine alone has no effect on these parameters.

Keywords

Arsenite  Taurine  NDRG-4  BDNF  SIRT-1  Neurotrophic 

Notes

Acknowledgments

This study was supported by the National Science Council, Taiwan, and the Center of Excellence for Marine Bioenvironment and Biotechnology.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Bekinschtein P, Cammarota M, Katche C, Slipczuk L, Rossato JI, Goldin A, Izquierdo I, Medina JH (2008) BDNF is essential to promote persistence of long-term memory storage. PNAS Neurosci 105:2711–2716CrossRefGoogle Scholar
  2. Bolla-Wilson K, Bleecker ML (1987) Neuropsychological impairment following inorganic arsenic exposure. J Occup Med 29:500–503PubMedGoogle Scholar
  3. Choi SC, Kim KD, Kim JT, Kim JW, Yoon DY, Choe YK, Chang YS, Paik SG, Lim JS (2003) Expression and regulation of NDRG2 (N-myc downstream regulated gene 2) during the differentiation of dendritic cells. FEBS Lett 553:413–418PubMedCrossRefGoogle Scholar
  4. Das J, Ghosh J, Manna P, Sinha M, Sil PC (2009) Arsenic-induced oxidative cerebral disorders: protection by taurine. Drug Chem Toxicol 32:93–102PubMedCrossRefGoogle Scholar
  5. Della Corte L, Crichton RR, Duburs G, Nolan K, Tipton KF, Tirzitis G, Ward RJ (2002) The use of taurine analogues to investigate taurine functions and their potential therapeutic applications. Amino Acids 23:367–380PubMedCrossRefGoogle Scholar
  6. Donmez G, Wang D, Cohen DE, Guarente L (2010) SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene ADAM10. Cell 142:320–332PubMedCrossRefGoogle Scholar
  7. Fukuda K, Hirai Y, Yoshida H, Hakajima T, Usii T (1982) Free-amino acid content of lymphocytes and granulocytes compared. Clin Chem 28:1758–1761PubMedGoogle Scholar
  8. Giasson BI, Sampathu DM, Wilson CA, Vogelsberg-Ragaglia V, Mushynski WE, Lee VM (2002) The environmental toxin arsenite induces tau hyperphosphorylation. Biochemistry 41:15376–15387PubMedCrossRefGoogle Scholar
  9. Hammes TO, Pedroso GL, Hartmann CR, Escobar TD, Fracasso LB, da Rosa DP, Marroni NP, Porawski M, da Silveira TR (2012) The effect of taurine on hepatic steatosis induced by thioacetamide. Dig Dis Sci 57:675–682PubMedCrossRefGoogle Scholar
  10. Hu B, Nikolakopoulou AM, Cohen-Cory S (2005) BDNF stabilizes synapses and maintains the structural complexity of optic axons in vivo. Development 132:4285–4298PubMedCrossRefGoogle Scholar
  11. Hughes MF (2002) Arsenic toxicity and potential mechanisms of action. Toxicol Lett 133:1–16PubMedCrossRefGoogle Scholar
  12. Idrissi A, Trenkner E (1999) Growth factors and taurine protect against excitotoxicity by stabilizing calcium homeostasis and energy metabolism. J Neurosci Res 19:9459–9468Google Scholar
  13. Lallemand F, De Witte Ph (2003) Taurine concentration in the brain and in the plasma following intraperitoneal injections. Amino Acids 26:111–116PubMedCrossRefGoogle Scholar
  14. Leon R, Wu H, Jin Y, Wei J, Buddhala C, Prentice H, Wu JY (2009) Protective function of taurine in glutamate-induced apoptosis in cultured neurons. J Neurosci Res 87:1185–1194PubMedCrossRefGoogle Scholar
  15. Louzada PR, Paula Lima AC, Mendonca-Silva DL, Noël F, De Mello FG, Ferreira ST (2004) Taurine prevents the neurotoxicity of β-amyloid and glutamate receptor agonists: activation of GABA receptors and possible implications for Alzheimer’s disease and other neurological disorders. FASEB J 18:511–518PubMedCrossRefGoogle Scholar
  16. Nagahara AH, Merrill DA, Coppola G, Tsukada S, Schroeder BE, Shaked GM, Wang L, Blesch A, Kim A, Conner JM, Rockenstein E, Chao MV, Koo EH, Geschwind D, Masliah E, Chiba AA, Tuszynski MH (2009) Neuroprotective effects of brain-derived neurotrophic factor in rodent and primate models of Alzheimer’s disease. Nat Med 15:331–337PubMedCrossRefGoogle Scholar
  17. Namgung U, Xia Z (2001) Arsenic induces apoptosis in rat cerebellar neurons via activation of JNK3 and p38 MAP kinases. Toxicol Appl Pharm 174:130–138CrossRefGoogle Scholar
  18. Ohki T, Hongo S, Nakada N, Maeda A, Takeda M (2002) Inhibition of neurite outgrowth by reduced level of NDRG4 protein in antisense transfected PC12 cells. Dev Brain Res 135:55–63CrossRefGoogle Scholar
  19. Okuda T, Higashi Y, Kokame K, Tanaka C, Kondoh H, Miyata T (2004) Ndrg1-deficient mice exhibit a progressive demyelinating disorder of peripheral nerves. Mol Cel Biol 24:3949–3956CrossRefGoogle Scholar
  20. Okuda T, Kokame K, Miyata T (2008) Differential expression patterns of NDRG family proteins in the central nervous system. J Histochem Cytochem 56:175–182PubMedCrossRefGoogle Scholar
  21. Perry G, Cash AD, Smith MA (2002) Alzheimer disease and oxidative stress. J Biomed Biotechnol 2:120–123PubMedCrossRefGoogle Scholar
  22. Phillips HS, Hains JM, Armanini M, Laramee GR, Johnson SA, Winslow JW (1991) BDNF mRNA is decreased in the hippocampus of individuals with Alzheimer’s disease. Neuron 7:7695–7702CrossRefGoogle Scholar
  23. Roy A, Sil PC (2012) Taurine protects murine hepatocytes against oxidative stress-induced apoptosis by tert-butyl hydroperoxide via PI3K/Akt and mitochondrial-dependent pathways. Food Chem 131:1086–1096CrossRefGoogle Scholar
  24. Schilling SH, Hjelmeland AB, Radiloff DR, Liu IM, Wakeman TP, Fielhauer JR, Foster EH, Lathia JD, Rich JN, Wang XF, Datto MB (2009) NDRG4 is required for cell cycle progression and survival in glioblastoma cells. J Biol Chem 284:25160–25169PubMedCrossRefGoogle Scholar
  25. Sidhu JS, Ponce RA, Vredevoogd MA, Yu X, Gribble E, Hong SW, Schneider E, Faustman EM (2006) Cell cycle inhibition by sodium arsenite in primary embryonic rat midbrain neuroepithelial cells. Toxicol Sci 89:475–484PubMedCrossRefGoogle Scholar
  26. Song F, Zhang L, Yu HX, Lu RR, Bao JD, Tan C, Sun Z (2012) The mechanism underlying proliferation-inhibitory and apoptosis-inducing effects of curcumin on papillary thyroid cancer cells. Food Chem 132:959–967CrossRefGoogle Scholar
  27. Tang BL, Chua CE (2008) SIRT1 and neuronal diseases. Mol Asp Med 29:187–200CrossRefGoogle Scholar
  28. Troy CM, Rabacchi SA, Xu Z, Maroney AC, Connors TJ, Shelanski ML, Greene LA (2001) Beta-amyloid-induced neuronal apoptosis requires c-Jun N-terminal kinase activation. J Neurochem 77:157–164PubMedCrossRefGoogle Scholar
  29. Vu KD, Carlettini H, Bouvet J, Cote J, Doyon G, Sylvain JF, Lacroix M (2012) Effect of different cranberry extracts and juices during cranberry juice processing on the antiproliferative activity against two colon cancer cell lines. Food Chem 132:959–967CrossRefGoogle Scholar
  30. Xi S, Jin Y, Lv X, Sun G (2010) Distribution and speciation of arsenic by transplacental and early life exposure to inorganic arsenic in offspring rats. Biol Trace Elem Res 134:84–97PubMedCrossRefGoogle Scholar
  31. Yamamoto H, Kokame K, Okuda T, Nakajo Y, Yanamoto H, Miyata T (2011) NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia. The J Biol Chem 286:26158–26165CrossRefGoogle Scholar
  32. Zhou RH, Kokame K, Tsukamoto Y, Yutani C, Kato H, Miyata T (2001) Characterization of the human NDRG gene family. A newly identified member, NDRG4, is specifically expressed in brain and heart. Genomics 73:86–97PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Chien-Te Chou
    • 1
  • Wen-Feng Lin
    • 1
  • Zwe-Ling Kong
    • 1
  • Shiow-Yi Chen
    • 2
  • Deng-Fwu Hwang
    • 1
    • 3
    • 4
    Email author
  1. 1.Department of Food ScienceNational Taiwan Ocean UniversityKeelung 202Taiwan, ROC
  2. 2.Institute of Bioscience and BiotechnologyNational Taiwan Ocean UniversityKeelungTaiwan, ROC
  3. 3.Department of Health and Nutrition BiotechnologyAsia UniversityTaichungTaiwan, ROC
  4. 4.Center of Excellence for Marine Bioenvironment and BiotechnologyNational Taiwan Ocean UniversityKeelungTaiwan, ROC

Personalised recommendations