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Amino Acids

, Volume 45, Issue 2, pp 383–391 | Cite as

Effects of dietary l-lysine intake on the intestinal mucosa and expression of CAT genes in weaned piglets

  • Liuqin He
  • Huansheng Yang
  • Yongqing Hou
  • Tiejun LiEmail author
  • Jun Fang
  • Xihong Zhou
  • Yulong YinEmail author
  • Li Wu
  • Martin Nyachoti
  • Guoyao Wu
Original Article

Abstract

The objective of this study was to evaluate effects of dietary l-lysine on the intestinal mucosa and expression of cationic amino acid transporters (CAT) in weaned piglets. Twenty-eight piglets weaned at 21 days of age (Duroc × Landrace × Yorkshire; 6.51 ± 0.65 kg body weight) were assigned randomly into one of the four groups: Zein + LYS (zein-based diet + 1.35 % supplemental lysine), Zein − LYS (zein-based diet), NF (nitrogen-free diet), and CON (basal diet). The experiment lasted for 3 weeks, during which food intake and body weight were recorded. At the end of the trial, blood was collected from the jugular vein of all pigs, followed by their euthanasia. Dietary supplementation with lysine enhanced villus height and crypt depth in the jejunum (P < 0.05). Jejunal mRNA levels for the b0,+-AT, y+LAT1 and CAT1 genes were greater (P < 0.05) in the Zein + LYS group than in the control, and the opposite was observed for CAT1. Dietary content of lysine differentially affected intestinal CAT expression to modulate absorption of lysine and other basic amino acids. Thus, transport of these nutrients is a key regulatory step in utilization of dietary protein by growing pigs and lysine in the diet influences the expression of amino acid transporters in the small intestine.

Keywords

Pigs Digestibility Cationic amino acids Intestinal mucosa Transporters 

Abbreviations

AA

Amino acids

CAT

Cationic amino acid transporters

Notes

Acknowledgments

This research was jointly supported by grants from National Basic Research Program of China (2013CB127301), National Natural Science Foundation of China (31272463, 31110103909), Hunan strategy emerging industry science research project (2011GK4061), the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists Grant No. 2011T2S15), and Texas A&M AgriLife Research Hatch project (H-8200).

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Blachier F, Mariotti F, Huneau JF et al (2007) Effects of amino acid derived luminal metabolites on the colonic epithelium and physiopathological consequences. Amino Acids 33:547–562PubMedCrossRefGoogle Scholar
  2. Bröer A, Wagner CA, Lang F et al (2000) The heterodimeric amino acid transporter 4F2hc/y + LAT2 mediates arginine efflux in exchange with glutamine. Biochem J 349:787–795PubMedGoogle Scholar
  3. Chen TS, Liou SY (2012) Amino acids with basic amino side chain accelerate ability of polyphenolic compounds. Food Chem 134:9–14CrossRefGoogle Scholar
  4. Chen LX, Yin YL, Jobgen WS et al (2007) In vitro oxidation of essential amino acids by intestinal mucosal cells of growing pigs. Livest Sci 109:19–23CrossRefGoogle Scholar
  5. Chen LX, Li P, Wang JJ et al (2009) Catabolism of nutritionally essential amino acids in developing porcine enterocytes. Amino Acids 37:143–152PubMedCrossRefGoogle Scholar
  6. Dai ZL, Zhang J, Wu G et al (2010) Utilization of amino acids by bacteria from the pig small intestine. Amino Acids 39:1201–1215PubMedCrossRefGoogle Scholar
  7. Dai ZL, Li XL, Xi PB et al (2012a) Metabolism of select amino acids in bacteria from the pig small intestine. Amino Acids 42:1597–1608PubMedCrossRefGoogle Scholar
  8. Dai ZL, Li XL, Xi PB et al (2012b) Regulatory role for l-arginine in the utilization of amino acids by pig small-intestinal bacteria. Amino Acids 43:233–244PubMedCrossRefGoogle Scholar
  9. Deng JP, Yang F, Yin YL et al (2010) Effects of digestible lysine levels on growth performance, serum metabolites and carcass composition in barrows. J Food Agric Environ 8:514–518Google Scholar
  10. François V, EllenI C, Carsten W et al (2004) CATs and HATs: the SLC7 family of amino acid transporter. Eur J Physiol 447:532–542CrossRefGoogle Scholar
  11. Fu WJ, Hu J, Spencer T et al (2006) Statistical models in assessing fold changes of gene expression in real-time RT-PCR experiments. Comput Biol Chem 30:21–26PubMedCrossRefGoogle Scholar
  12. Furuya WM, Graciano TS (2012) Digestible lysine requirement of Nile tilapia fingerlings fed arginine-to-lysine-balanced diets. Rev Bras Zootecn 41:485–490Google Scholar
  13. Garcia-Villalobos H, Morales-Trejo A (2012) Effects of dietary protein and amino acid levels on the expression of selected cationic amino acid transporters and serum amino acid concentration in growing pigs. Arch Anim Nutr 66:257–270PubMedCrossRefGoogle Scholar
  14. Gatrell SK, Berg LE, Barnard JT et al (2013) Tissue distribution of indices of lysine catabolism in growing swine. J Anim Sci 91:238–247PubMedCrossRefGoogle Scholar
  15. Gattas GF, Silva CD (2012) Dietary digestible lysine levels in diets for barrows from 60 to 100 days of age. Rev Bras Zootecn 41:91–97CrossRefGoogle Scholar
  16. Geng MM, Li TJ, Kong XF et al (2011) Reduced expression of intestinal N-acetylglutamate synthase in suckling piglets: a novel molecular mechanism for arginine as a nutritionally essential amino acid for neonates. Amino Acids 40:1513–1522PubMedCrossRefGoogle Scholar
  17. Gu XH, Li DF, She RP (2002) Effect of weaning on small intestinal structure and function in the piglet. Archiv für Tierernaehrung 56:275–286CrossRefGoogle Scholar
  18. Guo HX, Heinamaki J, Yliruusi J (2008) Stable aqueous film coating dispersion of Zero. J Colloid Interf Sci 322:478–484CrossRefGoogle Scholar
  19. Hampson DJ (1986) Alterations in piglet small intestinal structure at weaning. Res Vet Sci 40:32–40PubMedGoogle Scholar
  20. He QH, Kong XF, Wu GY et al (2009) Metabolomic analysis of the response of growing pigs to dietary l-arginine supplementation. Amino Acids 37:199–208PubMedCrossRefGoogle Scholar
  21. Hernández F, López M, Martínez S et al (2012) Effect of low-protein diets and single sex on production performance, plasma metabolites, digestibility, and nitrogen excretion in 1- to 48-day-old broilers. Poultry Sci 91:683–692CrossRefGoogle Scholar
  22. Hou YQ, Wang L, Zhang W et al (2012) Protective effects of N-acetylcysteine on intestinal functions of piglets challenged with lipopolysaccharide. Amino Acids 43:1233–1242PubMedCrossRefGoogle Scholar
  23. Kanai Y, Endou H (2003) Functional properties of multispecific amino acid transporters and their implications to transporter mediated toxicity. J Toxicol Sci 28:1–17PubMedCrossRefGoogle Scholar
  24. Kim SW, Wu G, Baker DH (2005) Ideal protein and dietary amino acid requirements for gestating and lactating sows. Pig News Inf 26:89–99Google Scholar
  25. Li TJ, Dai QZ, Yin YL et al (2008) Dietary starch sources affect net portal appearance of amino acids and glucose in growing pigs. Animal Consortium 2:723–729Google Scholar
  26. Li XL, Bazer FW, Gao HJ et al (2009) Amino acids and gaseous signaling. Amino Acids 37:65–78PubMedCrossRefGoogle Scholar
  27. Li XL, Rezaei R, Li P et al (2011a) Composition of amino acids in feed ingredients for animal diets. Amino Acids 40:1159–1168PubMedCrossRefGoogle Scholar
  28. Li FN, Yin YL, Tan BE et al (2011b) Leucine nutrition in animals and humans: mTOR signaling and beyond. Amino Acids 41:1185–1193PubMedCrossRefGoogle Scholar
  29. Mann EG, David LY, Sobrevia L (2003) Regulation of amino acid and glucose transporters in endothelial and smooth muscle cells. Physiol Rev 83:183–252PubMedGoogle Scholar
  30. National Research Council (NRC) (1998) Swine Nutrient requirements. National Academy of Science, Washington, DCGoogle Scholar
  31. Ren W, Yin YL, Liu G et al (2012) Effect of dietary arginine supplementation on reproductive performance of mice with porcine circovirus type 2 infection. Amino Acids 42:2089–2094PubMedCrossRefGoogle Scholar
  32. Rezaei R, Knabe DA, Li XL et al (2011) Enhanced efficiency of milk utilization for growth in surviving low-birth-weight piglets. J Anim Sci Biotech 2:73–83Google Scholar
  33. Rezaei R, Knabe DA, Tekwe CD et al (2013a) Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs. Amino Acids 44:911–923PubMedCrossRefGoogle Scholar
  34. Rezaei R, Wang WW, Wu ZL et al (2013b) Biochemical and physiological bases for utilization of dietary amino acids by young pigs. J Anim Sci Biotech 4:7CrossRefGoogle Scholar
  35. Russell H, Taylor PM, Hundal HS (2003) Amino acid transporters: roles in amino acid sensing and signaling in animal cells. Biochem J 373:1–18CrossRefGoogle Scholar
  36. Seow HF, Stefan B, Angelika B et al (2004) Hartnup disorder is caused by mutations in the gene encoding the neutral amino acid transporter SLC6A19. Nat Genet 36:1003–1007PubMedCrossRefGoogle Scholar
  37. Stein ED, Chang SD, Diamond JM (1987) Comparison of different dietary amino acids as inducers of intestinal amino acid transporter. Am J Physiol 274:232–239Google Scholar
  38. Sukhotnik I, Habib H, Jorge M et al (2005) Oral arginine improves intestinal recovery following ischemia–reperfusion injury in rat. Pediatr Surg Int 21:191–196PubMedCrossRefGoogle Scholar
  39. Tan B, Yin YL, Liu ZQ et al (2009) Dietary l-arginine supplementation increases muscle gain and reduces body fat mass in growing-finishing pigs. Amino Acids 37:169–175PubMedCrossRefGoogle Scholar
  40. Tan BE, Li XG, Wu G et al (2012) Dynamic changes in blood flow and oxygen consumption in the portal-drained viscera of growing pigs receiving acute administration of l-arginine. Amino Acids 43:2481–2489PubMedCrossRefGoogle Scholar
  41. Teng L, Lu CH, Zhu LG et al (2010) The biodegradation of zein in vitro and in vivo and its application in implants. Pharm Sci Tech. doi: 10.1208/s12249-010-9565-y Google Scholar
  42. Wang HJ, Lin ZX, Liu XM et al (2005) Heparin-loaded zero microsphere film and hemocompatibility. J Control Release 105:120–131PubMedCrossRefGoogle Scholar
  43. Wang JJ, Chen LX, Li P et al (2008) Gene expression is altered in piglet small intestine by weaning and dietary glutamine supplementation. J Nutr 138:1025–1032PubMedGoogle Scholar
  44. Wang XQ, Ou DY, Yin JD et al (2009a) Proteomic analysis reveals altered expression of proteins related to glutathione metabolism and apoptosis in the small intestine of zinc oxide-supplemented piglets. Amino Acids 37:209–218PubMedCrossRefGoogle Scholar
  45. Wang WW, Qiao SY, Li DF (2009b) Amino acids and gut function. Amino Acids 37:105–110PubMedCrossRefGoogle Scholar
  46. Wang WC, Gu WT, Tang XF et al (2009c) Molecular cloning, tissue distribution and ontogenetic expression of the amino acid transporter b0,+ cDNA in the small intestine of Tibetan suckling piglets. Comp Biochem Physiol 154:157–164Google Scholar
  47. Wang WW, Wu ZL, Dai ZL et al (2013) Glycine metabolism in animals and humans: implications for nutrition and health. Amino Acids. doi: 10.1007/s00726-013-1493-1 Google Scholar
  48. Wei JW, Carroll RJ, Harden KK et al (2012) Comparisons of treatment means when factors do not interact in two-factorial studies. Amino Acids 42:2031–2035PubMedCrossRefGoogle Scholar
  49. Wolfram S, Giering H, Scharrer E (1984) Na+-gradient dependence of basic amino acid transporter into rat intestinal brush border membrane vesicles. Biochem Physiol 78:475–480CrossRefGoogle Scholar
  50. Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37:1–17PubMedCrossRefGoogle Scholar
  51. Wu G (2010a) Recent advances in swine amino acid nutrition. J Anim Sci Biotech 1:49–61Google Scholar
  52. Wu G (2010b) Functional amino acids in growth, reproduction and health. Adv Nutr 1:31–37PubMedCrossRefGoogle Scholar
  53. Wu G (2013a) Amino acids: biochemistry and nutrition. CRC Press, Boca Raton, p 503CrossRefGoogle Scholar
  54. Wu G (2013b) Functional amino acids in nutrition and health. Amino Acids. doi: 10.1007/s00726-013-1500-6 Google Scholar
  55. Wu G, Meier SA, Knabe DA (1996) Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs. J Nutr 126:2578–2584PubMedGoogle Scholar
  56. Wu G, Bazer FW, Davis TA et al (2009) Arginine metabolism and nutrition in growth, health and disease. Amino Acids 37:153–168PubMedCrossRefGoogle Scholar
  57. Wu G, Wu ZL, Dai ZL et al (2013) Dietary requirements of “nutritionally nonessential amino acids” by animals and humans. Amino Acids 44:1107–1113PubMedCrossRefGoogle Scholar
  58. Yang CB, Albin DM, Wang ZR et al (2011) Apical Na+-D-glucose co-transporter 1 (SGLT1) activity and protein abundance are expressed along the jejunal crypt–villus axis in the neonatal pig. Am J Physiol Gastrointest Liver Physiol 300:60–70CrossRefGoogle Scholar
  59. Yao K, Li TJ, Huang RL et al (2011) Dietary l-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets. Br J Nutr 105:703–709PubMedCrossRefGoogle Scholar
  60. Yao K, Yin YL, Li XL et al (2012) Alpha-ketoglutarate inhibits glutamine degradation and enhances protein synthesis in intestinal porcine epithelial cells. Amino Acids 42:2491–2500PubMedCrossRefGoogle Scholar
  61. Yin YL, Huang RL, de Lange CFM et al (2004) Effect of including purified Jack Bean lectin in a casein based diet on apparent and true ileal amino acid digestibility in growing pigs. Anim Sci 79:283–291Google Scholar
  62. Yin FG, Zhang ZZ, Huang J et al (2010a) Digestion rate of dietary starch affects systemic circulation of amino acids in weaned pigs. Br J Nutr 103:1404–1412PubMedCrossRefGoogle Scholar
  63. Yin YL, Huang RL, Li TJ et al (2010b) Amino acid metabolism in the portal-drained viscera of young pigs: effects of dietary supplementation with chitosan and pea hull. Amino Acids 39:1581–1587PubMedCrossRefGoogle Scholar
  64. Yin YL, Yao K, Liu ZJ et al (2010c) Supplementing l-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs. Amino Acids 39:1477–1486PubMedCrossRefGoogle Scholar
  65. Yin FG, Yin YL, Li TJ (2011) Developmental changes of serum amino acids in suckling piglets. J Food Agric Environ 9:322–327Google Scholar
  66. Zhi AM, Zuo JJ, Zhou XY (2010) The Influence of different lysine concentration on the cationic amino acid transporter mRNA expression of porcine IEC. Chinese Agric Sci Bull 26:6–11Google Scholar
  67. Zhou XH, Wu X, Yin YL et al (2011) Preventive oral supplementation with glutamine and arginine has beneficial effects on the intestinal mucosa and inflammatory cytokines in endotoxemic rats. Amino Acids. doi: 10.1007/s00726-011-1137-2 Google Scholar

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Liuqin He
    • 1
  • Huansheng Yang
    • 1
  • Yongqing Hou
    • 2
  • Tiejun Li
    • 1
    Email author
  • Jun Fang
    • 3
  • Xihong Zhou
    • 4
  • Yulong Yin
    • 1
    • 2
    Email author
  • Li Wu
    • 1
  • Martin Nyachoti
    • 5
  • Guoyao Wu
    • 1
    • 6
  1. 1.Key Laboratory of Agroecology in Subtropical Region, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central China, Ministry of Agriculture, Hunan Engineering and Research Center for Animal and Poultry Sciences, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and PoultryThe Chinese Academy of ScienceChangshaChina
  2. 2.Hubei Key Laboratory of Animal Nutrition and Feed ScienceWuhan Polytechnic UniversityWuhanChina
  3. 3.College of Bioscience and BiotechnologyHunan Agricultural UniversityChangshaChina
  4. 4.Key Laboratory of Molecular Animal Nutrition, Institute of Feed ScienceZhejiang UniversityHangzhouChina
  5. 5.Department of Animal ScienceUniversity of ManitobaWinnipegCanada
  6. 6.Department of Animal ScienceTexas A&M UniversityCollege StationUSA

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