Effects of dietary l-lysine intake on the intestinal mucosa and expression of CAT genes in weaned piglets
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The objective of this study was to evaluate effects of dietary l-lysine on the intestinal mucosa and expression of cationic amino acid transporters (CAT) in weaned piglets. Twenty-eight piglets weaned at 21 days of age (Duroc × Landrace × Yorkshire; 6.51 ± 0.65 kg body weight) were assigned randomly into one of the four groups: Zein + LYS (zein-based diet + 1.35 % supplemental lysine), Zein − LYS (zein-based diet), NF (nitrogen-free diet), and CON (basal diet). The experiment lasted for 3 weeks, during which food intake and body weight were recorded. At the end of the trial, blood was collected from the jugular vein of all pigs, followed by their euthanasia. Dietary supplementation with lysine enhanced villus height and crypt depth in the jejunum (P < 0.05). Jejunal mRNA levels for the b0,+-AT, y+LAT1 and CAT1 genes were greater (P < 0.05) in the Zein + LYS group than in the control, and the opposite was observed for CAT1. Dietary content of lysine differentially affected intestinal CAT expression to modulate absorption of lysine and other basic amino acids. Thus, transport of these nutrients is a key regulatory step in utilization of dietary protein by growing pigs and lysine in the diet influences the expression of amino acid transporters in the small intestine.
KeywordsPigs Digestibility Cationic amino acids Intestinal mucosa Transporters
Cationic amino acid transporters
This research was jointly supported by grants from National Basic Research Program of China (2013CB127301), National Natural Science Foundation of China (31272463, 31110103909), Hunan strategy emerging industry science research project (2011GK4061), the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists Grant No. 2011T2S15), and Texas A&M AgriLife Research Hatch project (H-8200).
Conflict of interest
The authors declare that they have no conflict of interest.
- Deng JP, Yang F, Yin YL et al (2010) Effects of digestible lysine levels on growth performance, serum metabolites and carcass composition in barrows. J Food Agric Environ 8:514–518Google Scholar
- Furuya WM, Graciano TS (2012) Digestible lysine requirement of Nile tilapia fingerlings fed arginine-to-lysine-balanced diets. Rev Bras Zootecn 41:485–490Google Scholar
- Kim SW, Wu G, Baker DH (2005) Ideal protein and dietary amino acid requirements for gestating and lactating sows. Pig News Inf 26:89–99Google Scholar
- Li TJ, Dai QZ, Yin YL et al (2008) Dietary starch sources affect net portal appearance of amino acids and glucose in growing pigs. Animal Consortium 2:723–729Google Scholar
- National Research Council (NRC) (1998) Swine Nutrient requirements. National Academy of Science, Washington, DCGoogle Scholar
- Rezaei R, Knabe DA, Li XL et al (2011) Enhanced efficiency of milk utilization for growth in surviving low-birth-weight piglets. J Anim Sci Biotech 2:73–83Google Scholar
- Stein ED, Chang SD, Diamond JM (1987) Comparison of different dietary amino acids as inducers of intestinal amino acid transporter. Am J Physiol 274:232–239Google Scholar
- Wang WC, Gu WT, Tang XF et al (2009c) Molecular cloning, tissue distribution and ontogenetic expression of the amino acid transporter b0,+ cDNA in the small intestine of Tibetan suckling piglets. Comp Biochem Physiol 154:157–164Google Scholar
- Wu G (2010a) Recent advances in swine amino acid nutrition. J Anim Sci Biotech 1:49–61Google Scholar
- Yin YL, Huang RL, de Lange CFM et al (2004) Effect of including purified Jack Bean lectin in a casein based diet on apparent and true ileal amino acid digestibility in growing pigs. Anim Sci 79:283–291Google Scholar
- Yin FG, Yin YL, Li TJ (2011) Developmental changes of serum amino acids in suckling piglets. J Food Agric Environ 9:322–327Google Scholar
- Zhi AM, Zuo JJ, Zhou XY (2010) The Influence of different lysine concentration on the cationic amino acid transporter mRNA expression of porcine IEC. Chinese Agric Sci Bull 26:6–11Google Scholar